Characterization ofBorreliasp. isolated fromIxodes tanuki,I. turdus, andI. columnaein Japan by restriction fragment length polymorphism ofrrf(5S)-rrl(23S) intergenic spacer amplicons

Masuzawa, Toshiyuki; Komikado, Tetsuro; Iwaki, Atsue; Suzuki, Hiroyuki; Kaneda, Kazuhide; Yanagihara, Yasutake

doi:10.1111/j.1574-6968.1996.tb08411.x

Cited by 53 publications

(13 citation statements)

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“…garinii (26.2%) with prevalence rates in correspondence with data from neighboring regions Russia [40], Finland [41] and Latvia [38]. Several studies from Europe indicate a wide spread of B. afzelii and its subgroups NT28 and VS461 [19,25] in different regions of Europe and Asia as well as in different vector species [27,42]. The fact that both genetic groups of B .…”

Section: Discussionmentioning

confidence: 66%

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Borrelia burgdorferi sensu lato prevalence in tick populations in Estonia

et al. 2013

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BackgroundEstonia is located in a unique area of co-distribution of Ixodes ricinus and I. persulcatus, which are the main tick vectors of Borrelia burgdorferi sensu lato. In the last decade, the incidence rate of Lyme borreliosis in Estonia has increased dramatically up to 115.4 per 100,000 in 2012. Here we present the first survey of the presence, the prevalence and genetic characteristics of B. burgdorferi s.l. complex spirochetes in the tick population in Estonia.MethodsDuring the years 2006–2009, 2833 unfed Ixodes ricinus and I. persulcatus were collected from 43 sites in 7 counties in mainland Estonia as well as in 10 sites on the Saaremaa Island. DNA samples from ticks were analyzed individually using nested PCR of the ribosomal 5S-23S spacer region followed by bidirectional sequencing.ResultsThe overall estimated prevalence of B. burgdorferi s.l was 9.7% and varied from 4.9% to 24.2% on the mainland and to 10.7% in Saaremaa Island. Ixodes persulcatus ticks showed significantly higher prevalence rates compared to that in I. ricinus-16.3% and 8.2%, respectively. The most prevalent genospecies was B. afzelii which was detected in 53.5% of Borrelia-positive ticks, followed by B. garinii and B. valaisiana with 26.2% and 5.5%, respectively. Also, B. bavariensis and B. burgdorferi s.s. DNA in single I. ricinus ticks were detected. Borrelia afzelii, B. garinii and B. valaisiana were detected in both tick species. Two genetic subgroups of B. garinii (NT29 and 20047) and two genetic subgroups of B. afzelii (NT28 and VS461) were found to be circulating in all studied regions as well as in both tick species, except B. garinii subgroup NT29, which was found only in I. persulcatus ticks.ConclusionsIn the current study we detected the circulation of five B. burgdorferi s.l. genospecies and estimated the prevalence in ticks in different regions of Estonia. Detection and genetic characterization of Borrelia genospecies, especially those of public health importance, in the natural foci may help assessing high risk areas of human exposure to B. burgdorferi s.l.

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Section: Discussionmentioning

confidence: 66%

“…Analysis of nucleotide sequences showed that the investigated samples belong to two genomic subgroups of B . afzelii , VS461 and NT28 [25-27], that were detected in 62.6% and 28.6% of all B . afzelii positive ticks, respectively.…”

Section: Resultsmentioning

confidence: 99%

Borrelia burgdorferi sensu lato prevalence in tick populations in Estonia

et al. 2013

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“…The B. garinii NT29 group is widely spread not only in Western Siberia but in the Russian Far East (GenBank accession no. AY429014, AY429015), Japan ( 34 , 35 ), and China ( 36 ). The nested PCR with subsequent sequencing allowed us to detect DNA of B. garinii group NT29 in 3.6% ± 2.0% of D. reticulatus ticks.…”

Section: Discussionmentioning

confidence: 99%

Tickborne Pathogen Detection, Western Siberia, Russia

Рар

Фоменко

Dobrotvorsky

et al. 2005

Emerg. Infect. Dis.

136

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“…Representative DNA samples prepared from tick tissue were subjected to additional method for detection of B. burgdorferi s.l.rrf-rrl rDNA intergenic spacer PCR. Primers corresponding to the 3' end of 5S rDNA (rrf) (RIS1; 5'-CTG CGA GTT CGC GGG AGA-3' and RIS3; 5'-GGA GAG TAG GTT ATT GCC AGG-3') and the 5' end of 23S rDNA (rrl) (RIS2; 5'-TCC TAG GCA TTC ACC ATA-3' and RIS4; 5'-GAC TCT TAT TAC TTT GAC C-3') were used for fi rst-step (RIS1 and RIS2) and nested-PCR (RIS3 and RIS4) under the previously described PCR conditions (Masuzawa et al, 1996). Th e quality of extracted DNA was evaluated by PCR targeted to the internal transcribed spacer 2 (ITS2) region of tick-ribosomal DNA genes according to a previously described method (Radulović et al, 2010).…”

Section: Detection Of B Burgdorferi Sl Dnas In Tick Samples By Conventional Pcrmentioning

confidence: 99%

DETECTION OF BORRELIA SPIROCHETES IN TICKS WITH q16 REAL-TIME PCR

Stošić

Tomanović

Sukara

et al. 2021

AVM

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Lyme disease is a tick-borne disease caused by Borrelia burgdorferi s.l. spirochaetes. It is transmitted by several hard ticks of the genus Ixodes, mainly Ixodes ricinus in Europe. Higher environment temperature caused by climate changes is linked to a higher activity of ticks during most of the year, thus the importance of tick-borne diseases is increasing in the region. A total of 90 ticks were collected by removing from humans. Every tick collected was identified regarding the species. Total of 79 ticks belonging to the genus Ixodes were tested for the presence of the pathogen strains of Borrelia burgdorferi s.l, Borrelia afzelii, Borrelia garinii and Borrelia burgdoreri sensu stricto by a real-time PCR assay targeting the recA gene. In total, 8 of them tested positive. Representative samples were tested with conventional PCR and results were in accordance. This study showed that the Genesig q16 Real-Time PCR is an easy diagnostic test for fast detection of Borrelia spirochetes in ticks. Key words: Lyme disease, tick-borne disease, real-time PCR, Borrelia

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Characterization ofBorreliasp. isolated fromIxodes tanuki,I. turdus, andI. columnaein Japan by restriction fragment length polymorphism ofrrf(5S)-rrl(23S) intergenic spacer amplicons

Cited by 53 publications

References 23 publications

Borrelia burgdorferi sensu lato prevalence in tick populations in Estonia

Borrelia burgdorferi sensu lato prevalence in tick populations in Estonia

Tickborne Pathogen Detection, Western Siberia, Russia

DETECTION OF BORRELIA SPIROCHETES IN TICKS WITH q16 REAL-TIME PCR

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