DETECTION OF BORRELIA SPIROCHETES IN TICKS WITH q16 REAL-TIME PCR

Stošić, Marina Žekić; Tomanović, Snežana; Sukara, Ratko; Milosevic, Smiljana; Savić, Sara

doi:10.46784/eavm.v14i1.269

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…After washing ticks in 70% ethanol solution and afterwards in distilled water, the lysis of whole ticks in phosphate-saline buffer applying TissueLyser LT (Qiagen) was performed, with subsequent DNA extraction utilizing Genesig® Easy extraction kit (Primedesign Ltd), as described in Žekić Stošić et al (2021). The detection and quanti cation of Borrelia-DNA was performed using quantitative real-time PCR for RecA gene (Lyme Disease Genesig® Easy Kit, Primerdesign Ltd).…”

Section: Examination Of Ticks For the Presence Of Borreliamentioning

confidence: 99%

Bio-efficacy of permethrin/tetramethrin and lambda-cyhalothrin treatments in habitats of hard ticks (Acari, Ixodidae) populations with confirmed Borrelia spp. infection

et al. 2023

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The aim of the study was to evaluate the bio-e cacy of two different acaricides against mobile stages of hard ticks Ixodes ricinus, Dermacentor marginatus and Haemaphysalis punctata in their natural habitats. The study was conducted during 2020 and 2021 at localities populated by I. ricinus as the predominant species, at which the presence of Borrelia afzeli, B. garini and B.lusitaniae was con rmed. During the rst investigation year, a combination of two pyrethroids, permethrin and tetramethrin, with an insecticide synergist piperonyl butoxide (trade name: Perme Plus®) was tested. At the rst evaluation, 24h after the treatment with Perme Plus®, the e cacy expressed as reduction rate of the population density was within the interval of satisfying performance (70% − 90%) at all localities, while the highest e cacy (97.80%) was recorded on the 14th post-treatment day. In the second investigation year, the formulation based on lambdacyhalothrin (trade name: Icon® 10CS) was used. At the rst post-treatment evaluation day satisfying effects were also demonstrated. The highest recorded e cacy rate of lambda cyhalothrin (94.68%) was recorded on the 14th post-treatment day. Both tested acaricides manifested satisfying initial acaricidal effects against mobile stages of ticks and provided long-term effects. Comparison of the regression trend lines of population reduction revealed that satisfying effects of treatment with Perme Plus® lasted until the 17th post-treatment day, while the in the case of Icon® 10CS the residual effects were signi cantly prolonged (30 days).

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Section: Examination Of Ticks For the Presence Of Borreliamentioning

confidence: 99%

Bio-efficacy of permethrin/tetramethrin and lambda-cyhalothrin treatments in habitats of hard ticks (Acari, Ixodidae) populations with confirmed Borrelia spp. infection

et al. 2023

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“…The presence of B. burgdorferi s.l. was determined by real-time qPCR test, targeting the RecA gene, which was previously proven as a good genetic marker for Borrelia presence (Žeki c Stoši c et al, 2021). After sample preparation, ticks were washed in alcohol and rinsed in water.…”

Section: Molecular Analyses Of B Burgdorferi Using Pcrmentioning

confidence: 99%

Ecology and prevalence of Borrelia burgdorferi s.l. in Ixodes ricinus (Acari: Ixodidae) ticks

Ivanović

Stošić

Sabljić

et al. 2022

AVet

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Weather conditions greatly affect tick population densities and activity, on which depends the occurrence of tick-borne diseases (TBDs). During the spring months from 2017 to 2019, 1,357 specimens of Ixodes ricinus ticks were collected at 9 localities in the vicinity of Novi Sad (Serbia). The number of collected ticks varied considerably among the different sampling sites and years. Also, a statistically significant difference was found between months and observed number of ticks for each stadium. By statistical analysis of tick activity depending on microclimatic conditions, a positive and statistically significant relationship between temperature and the number of ticks for each life stage was established, but not for humidity. Dew had a statistically significant impact only on nymphs but not on adults. The infection rate of Borrelia burgdorferi s.l. was the highest in March (46.5–51.2%) and the lowest in May (32.9–34.8%). The highest prevalence was detected in males and the lowest in nymphs. Since there is a positive and statistically significant correlation between tick number and prevalence, the ability to provide weather-based predictions of the seasonal patterns of current tick activity is important for the risk assessment of TBDs such as Lyme borreliosis.

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Clinical Aspects and Detection of Emerging Rickettsial Pathogens: A “One Health” Approach Study in Serbia, 2020

Banović

Díaz-Sánchez

Simin³

et al. 2022

Front. Microbiol.

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Ticks carry numerous pathogens that, if transmitted, can cause disease in susceptible humans and animals. The present study describes our approach on how to investigate clinical presentations following tick bites in humans. To this aim, the occurrence of major tick-borne pathogens (TBPs) in human blood samples (n = 85) and the ticks collected (n = 93) from the same individuals were tested using an unbiased high-throughput pathogen detection microfluidic system. The clinical symptoms were characterized in enrolled patients. In patients with suspected TBP infection, serological assays were conducted to test for the presence of antibodies against specific TBPs. A field study based on One Health tenets was further designed to identify components of a potential chain of infection resulting in Rickettsia felis infection in one of the patients. Ticks species infesting humans were identified as Ixodes ricinus, Rhipicephalus sanguineus sensu lato (s.l.), Dermacentor reticulatus, and Haemaphysalis punctata. Five patients developed local skin lesions at the site of the tick bite including erythema migrans, local non-specific reactions, and cutaneous hypersensitivity reaction. Although Borrelia burgdorferi s.l., Babesia microti, Anaplasma phagocytophilum, and Candidatus Cryptoplasma sp. DNAs were detected in tick samples, different Rickettsia species were the most common TBPs identified in the ticks. The presence of TBPs such as Rickettsia helvetica, Rickettsia monacensis, Borrelia lusitaniae, Borrelia burgdorferi, Borrelia afzelii, A. phagocytophilum, and B. microti in ticks was further confirmed by DNA sequencing. Two of the patients with local skin lesions had IgG reactive against spotted fever group rickettsiae, while IgM specific to B. afzelii, Borrelia garinii, and Borrelia spielmanii were detected in the patient with erythema migrans. Although R. felis infection was detected in one human blood sample, none of the components of the potential chain of infection considered in this study tested positive to this pathogen either using direct pathogen detection in domestic dogs or xenodiagnosis in ticks collected from domestic cats. The combination of high-throughput screening of TBPs and One Health approaches might help characterize chains of infection leading to human infection by TBPs, as well as prevalence of emerging rickettsial pathogens in the Balkan region.

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DETECTION OF BORRELIA SPIROCHETES IN TICKS WITH q16 REAL-TIME PCR

Cited by 3 publications

References 21 publications

Bio-efficacy of permethrin/tetramethrin and lambda-cyhalothrin treatments in habitats of hard ticks (Acari, Ixodidae) populations with confirmed Borrelia spp. infection

Bio-efficacy of permethrin/tetramethrin and lambda-cyhalothrin treatments in habitats of hard ticks (Acari, Ixodidae) populations with confirmed Borrelia spp. infection

Ecology and prevalence of Borrelia burgdorferi s.l. in Ixodes ricinus (Acari: Ixodidae) ticks

Clinical Aspects and Detection of Emerging Rickettsial Pathogens: A “One Health” Approach Study in Serbia, 2020

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