Tickborne Pathogen Detection, Western Siberia, Russia

Рар, В. А.; Фоменко, Н. В.; Dobrotvorsky, Andrey K.; Ливанова, Н. Н.; Рудакова, С. А.; Fedorov, Evgeniy G.; Astanin, V. B.; Морозова, О. В.

doi:10.3201/eid1111.041195

Cited by 136 publications

(55 citation statements)

References 35 publications

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“…DNA was detected in questing and engorged nymphs and adults Ixodes spp. collected in North America, Europe, and Asia (13,(18)(19)(20)(21)(22)(23)(24)(25)(26). If one considers that ixodid ticks feed only once per stage, Bartonella spp.…”

mentioning

confidence: 99%

Transmission ofBartonella henselaebyIxodes ricinus

Cotté

Bonnet

Rhun

et al. 2008

Emerg. Infect. Dis.

224

198

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Bartonella spp. are facultative intracellular bacteria associated with several emerging diseases in humans and animals. B. henselae causes cat-scratch disease and is increasingly associated with several other syndromes, particularly ocular infections and endocarditis. Cats are the main reservoir for B. henselae and the bacteria are transmitted to cats by cat fl eas. However, new potential vectors are suspected of transmitting B. henselae, in particular, Ixodes ricinus, the most abundant ixodid tick that bites humans in western Europe. We used a membranefeeding technique to infect I. ricinus with B. henselae and demonstrate transmission of B. henselae within I. ricinus across developmental stages, migration or multiplication of B. henselae in salivary glands after a second meal, and transmission of viable and infective B. henselae from ticks to blood. These results provide evidence that I. ricinus is a competent vector for B. henselae.

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mentioning

confidence: 99%

Transmission ofBartonella henselaebyIxodes ricinus

Cotté

Bonnet

Rhun

et al. 2008

Emerg. Infect. Dis.

224

198

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“…Borreliae that cause Lyme borreliosis are transmitted by hard ticks of the genus Ixodes. I. persulcatus transmits only two human pathogenic Borrelia species in Novosibirsk region: B. afzelii and B. garinii (Postic et al, 1997;Korenberg et al, 2002;Livanova et al, 2003;Rar et al, 2005). In 1997, these two Borrelia species were detected for the first time in the territory of Novosibirsk region (Postic et al, 1997;Korenberg et al, 2002).…”

Section: Introductionmentioning

confidence: 90%

“…Internal primers designed by Postic et al (1994) were 5 0 -ctgcgagttcgcgggaga-3 0 (forward) and 5 0 -tcctaggcattcaccata-3 0 (reverse). A nested PCR was performed by the method described previously (Rar et al, 2005). A primer set 5 0 -gctggcagtgcgtcttaagcatgc-3 0 (forward, position 35-58 in B. burgdorferi s.s. B31 strain 16S rRNA gene) and 5 0 -gtgacgggcggtgtgtacaaggccc-3 0 (reverse, position 1343-1367) was used to amplify a fragment of the 16S rRNA gene as described previously (Fukunaga et al, 1996a).…”

Section: Dna Purification and Pcrmentioning

confidence: 99%

Diversity of Borrelia burgdorferi sensu lato in natural foci of Novosibirsk region

Фоменко¹,

Ливанова²,

Chernousova³

2008

International Journal of Medical Microbiology

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Ixodes persulcatus ticks, wild small mammals, and infected people in Novosibirsk region of Russia were investigated to analyze the local distribution and diversity of Borrelia burgdorferi sensu lato. The molecular typing was based on the partial sequencing of the 16S rRNA gene and the 5S-23S rRNA intergenic spacer region. The analysis of our data revealed the presence of two Borrelia species, B. garinii and B. afzelii. B. garinii DNA was found much more often, namely in 74% of all PCR-positive human blood samples, in 72% of all samples from small mammals (including blood and tissues), and in 76% of Borrelia-positive unfed adult I. persulcatus. B. afzelii DNA was found in 21% of the PCR-positive human blood samples, in 20% of the PCR-positive samples from the small mammals (including blood and tissues) and in 19% of the PCR-positive ticks. Mixed infection with B. garinii and B. afzelii was observed in both clinical and environmental samples. Interestingly, four samples isolated from I. persulcatus were closely related to the relapsing fever species B. miyamotoi.

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“…Taiga ticks collected in Tomsk region were also infected with Anaplasma phagocytophilum (2.4 ± 1.4%), Ehrlichia muris (8.8 ± 2.5%), and Bartonella spp. (37.6 ± 4.3%), agents of anaplasmosis, erlichiosis, and bartonellosis (Rar et al, 2005).…”

Section: Introductionmentioning

confidence: 99%