Characterization of human papillomavirus type 16 pseudovirus containing histones

Latar belakang Nyeri leher menyebabkan penurunan kekuatan otot, dan penurunan daya tahan otot sternocleidomastoid dan deep cervical flexor. Hal itu menyebabkan disfungsi mekanis dan nyeri kronis. Aktivasi deep cervical flexion muscle merupakan bentuk latihan penguatan pada otot, mampu memberikan efek yang signifikan dalam menurunkan nyeri leher. Tujuan penelitian ini bertujuan untuk mengetahui pengaruh pemberian deep neck flexor muscle activation pada nyeri leher Metode metode penelitian critical review dan desain studi randomized controlled trial menggunakan mesin pencarian literatur seperti Pubmed Central (PMC) dan Google Scholar, kemudian dilanjutkan dengan mengidentifikasi jenis Quartile (Q1-3) menggunakan Scimago Journal and Country Rank (SJR) dan penilaian dengan PEDro scale Hasil Sebanyak 9 artikel yang digunakan sebagai landasan penelitian, pada DCF menunjukan penurunan nyeri dengan VAS. Kesimpulannya penelitian menunjukkan adanya pengaruh yang signifikan pada latihan deep neck flexor muscle activation terhadap penurunan nyeri leher pada kondisi nyeri leher kronis maupun non spesifik. Pemberian deep neck flexor muscle activation yang terdiri dari craniocervical flexion, dan cervical stabilizating exercise. Latihan ini dilakukan dengan mendorong kepala kearah belakang dengan menjaga posisi kepala tetap lurus dan diberikan berupa tahanan hingga waktu yang sudah ditentukan Kata kunci nyeri leher, muscle activation, DFC

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“…Menjaga kekuatan otot-otot deep cervical flexor sangat penting untuk mengontrol postur dan stabilitas leher (Kim & Kwag, 2016).…”

Section: Pendahuluanunclassified

Pengaruh Pemberian Deep Neck Flexor Muscle Activation Terhadap Nyeri Leher: Critical Review

Apriliani¹,

Pristianto²,

Wijianto³

et al. 2021

FisioMu

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“…All this means that culture conditions, and also purification conditions, can affect the structural properties of VLPs. Moreover, the latter can decisively affect VLP immunogenicity and antigenicity (Mach et al 2006;Kim et al 2016). For these reasons, process design for VLPs should be carefully considered.…”

Section: Considerations Regarding Process Design For Yeast-derived Vlpsmentioning

confidence: 99%

Yeast as an expression system for producing virus-like particles: what factors do we need to consider?

Kim

2016

Lett Appl Microbiol

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The development of various types of virus-like particles (VLPs) has accelerated over the past two decades as the importance of VLPs for generating nextgeneration vaccines has been appreciated. Yeast has advantages such as scalable fermentation, low risk of contamination by adventitious agents, low production costs and the ability to produce VLPs with reliable qualities. It is generally recognized that yeast is suitable for producing VLPs that have simple structures and are produced intracellularly. However, recently there has been much effort to extend its applicability, and there is now evidence that it can be used as an expression platform for the productions of VLPs not only of nonenveloped viruses but also of enveloped viruses. Moreover, evidences indicated that yeast allows secretory VLP productions. Meanwhile, it has become evident that the quality and quantity of yeast-derived VLPs are influenced by the choice of plasmid and promoter, the ratio of the structural proteins produced. Here, we review the characteristics of the yeast expression system in terms of the production of VLP and compare it with other expression systems. We also consider strategies for VLP production in yeast and factors that need to be taken into account.

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“…The L1 major capsid protein spontaneously self-assembles into 72-pentamer virus-like particles (VLPs) (3). The L2 minor capsid protein is known to play a role in facilitating capsid assembly, enhancing infectivity, and HPV genome encapsulation (3)(4)(5). VLPs are empty particles composed of L1 protein alone or L1 and L2 proteins (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Peroxisomal Membrane Protein PMP34 Is Involved in the Human Papillomavirus Infection Pathway

et al. 2022

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Infection with high-risk human papillomavirus (HPV) types is linked to the onset of several cancers. The mechanism of HPV infection, however, has not yet been fully elucidated. Here, using the newly developed HPV infectious pseudovirion (HPV PsV) and a genome-wide clustered regularly interspaced short palindromic repeat (CRISPR) screening system, we established an experimental system and searched for genes involved in HPV infection. The HPV PsV has the truncated herpes simplex virus thymidine kinase (dTK) to kill PsV-infected cells when combined with ganciclovir. The five rounds of selection of 293FT cells by infection with HPV PsVs identified two candidate genes involved in the HPV infection pathway. The validation experiments showed that SLC25A17, which encodes the peroxisomal membrane protein PMP34, was involved in the HPV infection pathway. The gRNAs against SLC25A17 attenuated the efficiency of HPV PsV infection in 293FT and HeLa cells. Although further experiments are required to determine whether PMP34 acts as the HPV infection pathway, these results indicate that our screening system is useful for identification of the genes involved in the HPV infection pathway.

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Characterization of human papillomavirus type 16 pseudovirus containing histones

Cited by 7 publications

References 38 publications

Pengaruh Pemberian Deep Neck Flexor Muscle Activation Terhadap Nyeri Leher: Critical Review

Pengaruh Pemberian Deep Neck Flexor Muscle Activation Terhadap Nyeri Leher: Critical Review

Yeast as an expression system for producing virus-like particles: what factors do we need to consider?

Peroxisomal Membrane Protein PMP34 Is Involved in the Human Papillomavirus Infection Pathway

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