Characterization of galactoglucomannan extracted from spruce (Picea abies) by heat-fractionation at different conditions

Lundqvist, Jon; Jacobs, Anna; Palm, Magnus; Zacchi, Guido; Dahlman, Olof; Stålbrand, Henrik

doi:10.1016/s0144-8617(02)00111-x

Cited by 96 publications

(67 citation statements)

References 18 publications

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“…Several reports showed that about 60-65 % of mannose residues in galactoglucomannan from native Norway spruce wood and pulp were acetylated at either the C-2 or C-3 position (Aspinall et al 1962 ;Popa and Spiridon 1998 ;Timell 1967 ;Willfor et al 2003 ). Lundqvist et al ( 2002Lundqvist et al ( , 2003 extracted galactoglucomannan from spruce ( Picea abies ) by heat fractionation at different temperatures and characterized it. Galactoglucomannan from spruce contained about one third of D-mannosyl units substituted by O -acetyl groups with an equal distribution between C-2 and C-3 and a molar ratio of 0.1:1:4 (galactose/glucose/mannose).…”

Section: Mannan: Occurrence and Structurementioning

confidence: 99%

Microbial Mannanases: Properties and Applications

Soni

Kango

2013

Advances in Enzyme Biotechnology

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Mannans are a major constituent of the hemicellulose fraction of lignocelluloses. Mannans perform distinct functions as structural components in cell walls of softwoods and storage functions in seeds. Enzymatic hydrolysis of mannan involves the backbone hydrolyzing endo-β-mannanases and β-mannosidases. Mannans are heteropolymeric and their hydrolysis also requires the action of β-glucosidases and side-chain cleaving α-galactosidases and acetyl mannan esterases. Microorganisms are therefore explored for the production of such repertoire of enzymes so that effective mannan hydrolysis can be achieved. The present chapter discusses the occurrence and structural properties of mannans in plant materials and its hydrolysis using enzymes sourced from various fungi and other microorganisms. The production and properties of mannanolytic enzymes, their cloning and expression in heterologous hosts, and their application have also been discussed .

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Section: Mannan: Occurrence and Structurementioning

confidence: 99%

Microbial Mannanases: Properties and Applications

Soni

Kango

2013

Advances in Enzyme Biotechnology

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“…Dextran molecular mass standards (Fluka Chemie) and maltose (molecular mass 342 kD; Sigma) were also applied and eluted as calibration standards. The weight-averaged molecular mass of xyloglucan was calculated according to Lundqvist et al (2003), and the reproducibility of the resulting patterns was assessed by analyzing several technical replicates.…”

Section: Size Exclusion Chromatographymentioning

confidence: 99%

Xyloglucanendo-Transglycosylase-Mediated Xyloglucan Rearrangements in Developing Wood of Hybrid Aspen

et al. 2010

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Xyloglucan endo-transglycosylases (XETs) encoded by xyloglucan endo-transglycosylases/hydrolase (XTH) genes modify the xyloglucan-cellulose framework of plant cell walls, thereby regulating their expansion and strength. To evaluate the importance of XET in wood development, we studied xyloglucan dynamics and XTH gene expression in developing wood and modified XET activity in hybrid aspen (Populus tremula × tremuloides) by overexpressing PtxtXET16-34. We show that developmental modifications during xylem differentiation include changes from loosely to tightly bound forms of xyloglucan and increases in the abundance of fucosylated xyloglucan epitope recognized by the CCRC-M1 antibody. We found that at least 16 Populus XTH genes, all likely encoding XETs, are expressed in developing wood. Five genes were highly and ubiquitously expressed, whereas PtxtXET16-34 was expressed more weakly but specifically in developing wood. Transgenic up-regulation of XET activity induced changes in cell wall xyloglucan, but its effects were dependent on developmental stage. For instance, XET overexpression increased abundance of the CCRC-M1 epitope in cambial cells and xylem cells in early stages of differentiation but not in mature xylem. Correspondingly, an increase in tightly bound xyloglucan content was observed in primary-walled xylem but a decrease was seen in secondary-walled xylem. Thus, in young xylem cells, XET activity limits xyloglucan incorporation into the tightly bound wall network but removes it from cell walls in older cells. XET overexpression promoted vessel element growth but not fiber expansion. We suggest that the amount of nascent xyloglucan relative to XET is an important determinant of whether XET strengthens or loosens the cell wall.

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“…Esto último, es la causa principal en la diferente composición de las hemicelulosas, lo que da como resultado los diferentes procesos de volatilización. De hecho, la hemicelulosa de las latifoliadas está compuesta principalmente de 80-90% de 4-O-metylglucoronoxilano (Tumuluru et al 2011), mientras que las coníferas presentan entre 60-70% de glucomanano y del 15-30% de arabinogalactano como sus principales constituyentes (Lundqvist et al 2003). Desde el punto de vista cinético, la tasa de pérdida másica de la hemicelulosa en las latifoliadas, aumenta proporcionalmente con la temperatura de operación mostrando un máximo de 290°C; muy diferente a lo que sucede con la hemicelulosa de las coníferas estudiadas.…”

Section: Pinus Sylvestrisunclassified

Estudio de cinética en procesos termogravimétricos de materiales lignocelulósicos

Flores

Rutiaga-Quiñones

2018

Maderas, Cienc. tecnol.

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RESUMENLa madera de pino representa el 20% de las especies forestales plantadas en todo el mundo, y actualmente es de vital importancia en aplicaciones industriales como en aserrío, fabricación de papel y medicina. Actualmente, se ha destacado su uso en aplicaciones energéticas. En este estudio se presenta una breve revisión sobre el análisis térmico realizado a diversas especies de madera de pino. Se enfoca principalmente al análisis de los resultados respecto a cómo varía el contenido de masa o pérdida de peso con el cambio de temperatura a partir del análisis de las curvas de termogravimetría y su derivada. Se considerarón diversas especies de madera de pino: Pinus caribaea, Pinus patula, Cupressus sempervirens, Pinus pinaster, Pinus radiata, Pinus sylvestris, Pinus pinea y Pinus taeda.Con el objetivo de mostrar las diferencias y similitudes respecto a la estabilidad térmica de las especies mencionadas, se han discutido los principales parámetros a partir de las curvas de termogravimetría como energía de activación, factor pre-exponencial y orden de reacción. Además, se examinan las etapas de descomposición de acuerdo a los principales componentes de la madera tales como agua, celulosa, hemicelulosa, lignina y extractivos.Palabras clave: Conversión térmica, Cupressus sempervirens, energía renovable, parámetros cinéticos, Pinus sp. ABSTRACTPine wood accounts for 20% of the world's planted forest species, and is currently of vital importance in industrial applications such as sawmills, papermaking and medicine. Actually, its use in energy applications has been emphasized. This study presents a brief review on the thermal analysis of various species of pine wood. We focus mainly on the analysis of the results regarding how the mass content or weight loss varies with the temperature change from the analysis of the thermogravimetric curves and its derivative. Several species of pine wood have been considered: Pinus caribaea, Pinus patula, Cupressus sempervirens, Pinus pinaster, Pinus radiata, Pinus sylvestris, Pinus pinea and Pinus taeda. In order to show the differences and similarities with respect to the thermal stability of the mentioned species, we have discussed the main parameters from the thermogravimetry curves such as activation energy, pre-exponential factor and reaction order. In addition, the decomposition stages are examined according to the main components of the wood such as water, cellulose, hemicellulose, lignin and extractives.

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Characterization of galactoglucomannan extracted from spruce (Picea abies) by heat-fractionation at different conditions

Cited by 96 publications

References 18 publications

Microbial Mannanases: Properties and Applications

Microbial Mannanases: Properties and Applications

Xyloglucanendo-Transglycosylase-Mediated Xyloglucan Rearrangements in Developing Wood of Hybrid Aspen

Estudio de cinética en procesos termogravimétricos de materiales lignocelulósicos

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