Microbial Mannanases: Properties and Applications

Soni, Hemant; Kango, Naveen

doi:10.1007/978-81-322-1094-8_4

Cited by 16 publications

(12 citation statements)

References 83 publications

(64 reference statements)

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“…A more eco-friendly and sustainable potential strategy for the hydrolysis and solubilization of coffee polysaccharides is to use an enzymatic route, since enzymes act under moderate process conditions and are highly specific. The main enzymes involved in degradation of the mannan structures are β-mannanase, β-mannosidase, β-glycosidase, and α-galactosidase [7]. Mannanases can be produced by a wide range of microorganisms, including different fungi [8][9][10][11] and bacteria [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing

et al. 2020

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Soluble coffee offers the combined benefits of high added value and practicality for its consumers. The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts. This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing. Different substrate compositions were studied, an ideal microorganism was selected, and the fermentation conditions were optimized. Cultivations for enzymes production were carried out in flasks and in a packed-bed bioreactor. Higher enzyme yield was achieved in the bioreactor, due to better aeration of the substrate. The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillus niger F12. The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee. The addition of a commercial cellulase cocktail to the crude extract increased the hydrolysis yield by 56%. The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.

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Section: Introductionmentioning

confidence: 99%

β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing

et al. 2020

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“…Owing to the beneficial effects of pretreatment, mannanases have been widely used in various industries like food and nutraceutical industries (production of prebiotics), paper and pulp industry (enzymatic bleaching), detergent industry, poultry feed industry (feed upgradation) etc. (Dhawan and Kaur 2007; Soni and Kango 2013). Large amounts of agro-industrial lignocellulosic waste are generated and accumulated from various industrial activities that cause environmental pollution.…”

Section: Introductionmentioning

confidence: 99%

“…Endo-1, 4-β-D-mannanase (EC 3.2.1.78) is a hemicellulolytic enzyme that randomly hydrolyses 1,4-β-D-mannopyranosyl linkages within the main chains of mannans and heteropolysaccharides such as galactomannan, glucomannan and galactoglucomannan. Other accessory enzymes involved in mannan breakdown include β-mannosidase, β-glucosidase and α-galactosidase that liberate mannose, glucose and galactose from mannan (Dhawan and Kaur 2007; Soni and Kango 2013). Owing to the beneficial effects of pretreatment, mannanases have been widely used in various industries like food and nutraceutical industries (production of prebiotics), paper and pulp industry (enzymatic bleaching), detergent industry, poultry feed industry (feed upgradation) etc.…”

Section: Introductionmentioning

confidence: 99%

Experimental design of response surface methodology used for utilisation of palm kernel cake as solid substrate for optimised production of fungal mannanase

et al. 2016

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The results obtained from this work strongly indicate that the solid state fermentation (SSF) system using the palm kernel cake (PKC) as a substrate is an economical method for the production of β-mannanase at extremely low operational cost based on the fact that PKC is one of the cheap and abundant agro-waste by-products of the palm oil industry. Under initial conditions, i.e. 2 mm particle size of PKC, the moisture ratio of 1:1 of PKC:moistening agent and pH 7, Malbranchea cinnamomea NFCCI 3724 produced 109 U/gram distribution of the substrate (gds). The production of β-mannanase was optimised by the statistical approach response surface methodology (RSM) using independent variables, namely initial moisture (12.5), pH (9.0) and solka floc (100 mg). Noticeably, six fold enhancement of β-mannanase production (599 U/gds) was obtained under statistically optimised conditions. HPLC results revealed that β-mannanase is an endo-active enzyme that generated manno-oligosaccharides with a degree of polymerisation (DP) of 3 and 4. Semi-native PAGE analysis revealed that M. cinnamomea produced three isoforms of mannanase. Selective production of oligosaccharide makes M. cinnamomea β-mannanase an attractive enzyme for use in food and nutraceutical industries.

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“…Complete degradation of mannan requires synergistic action of multiple enzymes viz. β‐mannanases (1,4‐β‐mannan mannohydrolase EC 3.2.1.78) that release mannooligosaccharides (MOS), β‐mannosidases (1,4‐β‐mannopyranoside hydrolase EC 3.2.1.25) that release mannose, β‐glucosidases (1,4‐β‐glucoside glucohydrolase EC 3.2.1.21) that break glycosidic bond between mannose and glucose and side‐chain cleaving enzyme, α‐galactosidase (1,6‐α‐galactoside galactohydrolase EC 3.2.1.22) .…”

Section: Introductionmentioning

confidence: 99%

“…Complete degradation of mannan requires synergistic action of multiple enzymes viz. β-mannanases (1,4- [6,7].…”

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confidence: 99%

Screening, statistical optimized production, and application of β‐mannanase from some newly isolated fungi

Soni

Rawat

Ahirwar

et al. 2016

Engineering in Life Sciences

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Eighty‐eight fungi isolated from soil and decaying organic matter were screened for mannanolytic activity. Twenty‐eight fungi produced extracellular mannanase on locust bean gum as evidenced by zone of hydrolysis produced on mannan agar gel. Six prominent producers, including four Fusarium species namely Fusarium fusarioides NFCCI 3282, Fusarium solani NFCCI 3283, Fusarium equiseti NFCCI 3284, Fusarium moniliforme NFCCI 3287 with Cladosporium cladosporioides NFCCI 3285 and Acrophialophora levis NFCCI 3286 produced the β‐mannanase in the range of 84–140 nkat/mL. All these grew well on particulate substrates in solid‐state fermentation (SSF), producing relatively higher titers on mannan‐rich palm kernel cake (PKC) and copra meal. Two high yielding strains, F. equiseti (1747 nkat/gds) and A. levis (897 nkat/gds) were selected for statistical optimization of mannanase on PKC. Interaction of two critical solid state fermentation parameters, pH and moisture on mannanase production by these two molds was studied by response surface method. Optimized production on PKC resulted in three‐ to fourfold enhancement in enzyme yield was observed in case of F. equiseti (5945 nkat/gds) and A. levis (4726 nkat/gds). HPLC analysis of mannan hydrolysate indicated that F. equiseti and A. levis mannanase performed efficient hydrolysis of konjac gum (up to 99%) with exclusive mannooligosaccahride (DP of 4) production. A seminative SDS‐PAGE revealed that A. levis and F. solani produced three isoforms, F. moniliforme produced two isoforms while F. fusarioides, F. equiseti, and C. cladosporioides produced a single enzyme.

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Microbial Mannanases: Properties and Applications

Cited by 16 publications

References 83 publications

β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing

β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing

Experimental design of response surface methodology used for utilisation of palm kernel cake as solid substrate for optimised production of fungal mannanase

Screening, statistical optimized production, and application of β‐mannanase from some newly isolated fungi

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