Characterization of endometrial mesenchymal stem-like cells obtained by endometrial biopsy during routine diagnostics

Human CD133+ bone marrow-derived stem cells promote endometrial proliferation in a murine model of Asherman syndrome

“…A new functionalis layer grows, differentiates, and decidualizes every month after the basal layer. This remarkable remodeling process occurs around 400 times during the woman's reproductive lifetime and may even extend beyond the reproductive years with the use of hormone replacement therapy due to endometrial somatic stem cell activity (3)(4)(5)(6)(7)(8)(9)(10).…”

Endometrial reconstruction from stem cells

“…Human endometrium is the only source of tissue-derived MSC that can be isolated with minimal invasiveness without the need for an anesthetic. The W5C5 þ cells are located in the functionalis (and basalis) and can be retrieved by endometrial biopsy, as MSC-like cells have been demonstrated in endometrial biopsies (55,57). In summary, W5C5 þ and CD146 þ PDGF-Rb þ are essentially the same subpopulation of endometrial stromal cells (56) that have MSC properties and phenotype as defined by the International Society for Cellular Therapy (20).…”

The Endometrium as a Source of Mesenchymal Stem Cells for Regenerative Medicine1

“…Characterization of endometrial stromal cells revealed mesenchymal stem cell properties of these cells through clonogenicity assays, adherence to plastic, fibroblast-like morphology, and differentiation to adipogenic, chondrogenic and osteogenic fates in vitro [8,9,[21][22][23][24][25][26]. Further characterization of human endometrial stromal cells using antibody panels showed the expression of CD29, CD44, CD73, CD90, CD105, MSI1 and NOTCH1 [8,22,25]. Others have suggested that endometrial stem cells are c-kit (CD117) and CD34 expressing cells in the basalis layer of endometrium [27].…”