The Endometrium as a Source of Mesenchymal Stem Cells for Regenerative Medicine1

Mutlu, Levent; Hufnagel, Demetra; Taylor, Hugh S.

doi:10.1095/biolreprod.114.126771

Cited by 84 publications

(79 citation statements)

References 88 publications

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“…As an alternative source of human stem cells, the newly identified MenSCs exhibit their potential for ASC‐based therapy. Due to the natural high richness and non‐invasive sample collection, MenSCs display tempting features as replacement of bone marrow‐derived stem cells .…”

Section: Discussionmentioning

confidence: 99%

Biological characteristics of human menstrual blood‐derived endometrial stem cells

Niu

Yang

et al. 2017

J Cellular Molecular Medi

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Successful isolation of human endometrial stem cells from menstrual blood, namely menstrual blood‐derived endometrial stem cells (MenSCs), has provided enticing alternative seed cells for stem cell‐based therapy. MenSCs are enriched in the self‐regenerative tissue, endometrium, which shed along the periodic menstrual blood and thus their acquisition involves no physical invasiveness. However, the impact of the storage duration of menstrual blood prior to stem cell isolation, the age of the donor, the number of passages on the self‐renewing of MenSCs, the paracrine production of biological factors in MenSCs and expression of adhesion molecules on MenSCs remain elusive. In this study, we confirmed that MenSCs reside in shedding endometrium, and documented that up to 3 days of storage at 4°C has little impact on MenSCs, while the age of the donor and the number of passages are negatively associated with proliferation capacity of MenSCs. Moreover, we found that MenSCs were actually immune‐privileged and projected no risk of tumour formation. Also, we documented a lung‐ and liver‐dominated, spleen‐ and kidney‐involved organic distribution profile of MenSC 3 days after intravenous transfer into mice. At last, we suggested that MenSCs may have potentially therapeutic effects on diseases through paracrine effect and immunomodulation.

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Section: Discussionmentioning

confidence: 99%

Biological characteristics of human menstrual blood‐derived endometrial stem cells

Niu

Yang

et al. 2017

J Cellular Molecular Medi

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“…Further, down‐regulation of Let‐7 miRNAs has been reported in breast tumour‐initiating cells (BT‐IC), and restoration of Let‐7 in these cells caused reduced proliferation and mammosphere formation in vitro as well as tumour formation and metastasis in NOD/SCID mice . We, previously identified stem cells in endometrium and endometriosis . It will be interesting to see whether Let‐7 also regulates stem cells in endometriosis in a similar manner to regulation of BT‐IC in breast cancers.…”

Section: Discussionmentioning

confidence: 81%

“…38 We, previously identified stem cells in endometrium and endometriosis. [39][40][41] It will be interesting to see whether Let-7 also regulates stem cells in endometriosis in a similar manner to regulation of BT-IC in breast cancers. In our model, ER-α expression was more strongly suppressed than ER-β with Let-7b miRNA suggesting that Let-7 blocks oestrogen stimulation in the treatment of endometriosis.…”

Section: Treatment Of Endometriosis By Intraperitoneal Injections Of mentioning

confidence: 99%

microRNA Let‐7b: A Novel treatment for endometriosis

Şahin

Mamillapalli

et al. 2018

J Cellular Molecular Medi

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Endometriosis is an oestrogen‐dependent, chronic inflammatory disease that affects 10% of reproductive‐aged women. Current treatment options depend on female sex steroid hormone modulation; however, all have side effects and are not useful in women who want to conceive. microRNAs treatments have provided promising results for some chronic diseases and cancers. We have previously shown the microRNA Let‐7b is repressed in endometriosis and that loss of Let‐7 contributes to the pathophysiology of the disease. Here, we propose using microRNA Let‐7b for the treatment of endometriosis in a murine model. Endometriosis was treated using microRNA Let‐7b or a scrambled control microRNA. Let‐7b treatment resulted in reduced endometriosis lesion size. Decreased gene expression was noted in several genes known to promote endometriosis growth including ER‐α, ER‐ß, Cyp19a, KRAS 4A, KRAS 4B and IL‐6. These results indicate that microRNA Let‐7b has a pleiotropic role in endometriosis pathophysiology affecting oestrogen signalling, inflammation and growth factor receptors. Local treatment of endometriosis with Let‐7b is a promising therapy for endometriosis that simultaneously affects multiple pathways driving endometriosis without systemic hormonal side effects.

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“…Adjacent to the blood vessels exhibit pericytic characteristics. (Masuda, Anwar, Buhring, Rao, & Gargett, ; Mutlu et al., ; Schwab & Gargett, ; Spitzer et al., ). Our immunofluorescence study demonstrates that perivascular compartment in pig endometrium may constitute a major source of candidate mesenchymal stem/progenitor cells due to the presence of typical set of MSC markers in resident cells.…”

Section: Discussionmentioning

confidence: 99%

“…Evidence indicates that endometrial stem cells may have a roles in the development and progression of human proliferative disorders such as endometriosis and endometrial cancers (Gargett et al., ). On the other hand, the endometrial‐derived stem‐like cells are a promising source of therapeutic cells due to their high regenerative potential, angiogenic and immunomodulatory activities, and easy availability by endometrial biopsies (Mutlu, Hufnagel, & Taylor, ; Verdi, Tan, Shoae‐Hassani, & Seifalian, ). Hence, advancing our knowledge of the stem cells and their niches is important for better understanding of the mechanisms of endometrial homeostasis and pathology.…”

Section: Introductionmentioning

confidence: 99%

Identification of perivascular and stromal mesenchymal stem/progenitor cells in porcine endometrium

Wiater

Niedziela

Posmysz

et al. 2017

Reprod Domestic Animals

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Mammalian uterus contains a population of mesenchymal stem/progenitor cells that likely contribute to endometrial regeneration during each reproductive cycle. In human and mouse, they reside in perivascular, epithelial and stromal compartments of the endometrial functionalis and basalis. Here, we aimed to identify tissue resident cells expressing mesenchymal stem cell markers CD29, CD44, CD90, CD105, CD140b and CD146 in the porcine endometrium. We used single immunofluorescence and Western blotting. Each of these markers was detected in small cells surrounding endometrial blood vessels. CD105 and CD146 were also expressed in single stromal cells. A few stromal and perivascular cells showed the presence of pluripotency marker Oct4 in the cytoplasm, but not in the nucleus, which may imply they are not truly pluripotent. Endometrial cell cultures were examined for the expression of CD29, CD44, CD90, CD105 and CD140b proteins and tested in wound-healing assay and culture model of chemotaxis. In conclusion, our results demonstrate perivascular location of prospective mesenchymal stem/progenitor cells in the porcine endometrium and may suggest that stromal CD105 and CD146 cells represent more mature precursors originating from their perivascular ancestors.

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The Endometrium as a Source of Mesenchymal Stem Cells for Regenerative Medicine1

Cited by 84 publications

References 88 publications

Biological characteristics of human menstrual blood‐derived endometrial stem cells

Biological characteristics of human menstrual blood‐derived endometrial stem cells

microRNA Let‐7b: A Novel treatment for endometriosis

Identification of perivascular and stromal mesenchymal stem/progenitor cells in porcine endometrium

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