Characterization of embryogenesis-related proteins in alfalfa (Medicago sativa)

Giroux, Randal W.; Pauls, K. Peter

doi:10.1034/j.1399-3054.1996.960406.x

Cited by 9 publications

(10 citation statements)

References 18 publications

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“…Kinases have been identified with the role of transduction of the signal from the cell membrane to the action site. These protein kinases are activated by autophosphorylation and are involved in regulation of other successive transducers in the signal transduction pathway, as seen with alfalfa somatic embryo genes (ASET2) (Giroux and Pauls, 1997). The clones identified in our study, which encode Ser/Thr protein kinaselike protein, may be involved in cellular communication or signal transduction in SE.…”

Section: Several Major Types Of Signaling Molecules Are Involved In Sementioning

confidence: 91%

“…The first evidence of differential gene expression during this developmental process was reported in the 1980s (Sung and Okimoto, 1981), and to date, some genes that are activated or differentially expressed during the induction and development of somatic embryos have been cloned and studied by use of various molecular techniques (Giroux and Pauls, 1997;Schmidt et al, 1997;Kwon et al, 2004). Nonetheless, most studies have concentrated on the later stages of embryogenesis (Zimmerman, 1993), although most vital genes are expressed at high levels at the time of SE initiation.…”

Section: Introductionmentioning

confidence: 97%

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Isolation and Characterization of Genes Associated to Cotton Somatic Embryogenesis by Suppression Subtractive Hybridization and Macroarray*

et al. 2006

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Somatic embryogenesis (SE) is the developmental reprogramming of somatic cells toward the embryogenesis pathway and is a notable illustration of cell totipotency. To identify genes involved in SE, subtractive polymerase chain reaction (PCR) was performed to generate transcripts highly enriched for SE-related genes, using cDNA prepared from a mixture of embryogenic callus and pre-globular somatic embryos, as the tester, and cDNA from non-embryogenic callus, as the driver. After differential screening and subsequent confirmation by reverse Northern blot analysis, a total of 671 differentially expressed cDNA fragments were identified, and 242 uni-genes significantly up-regulated during cotton SE were recovered, as confirmed by Northern blot and reverse-transcription PCR analysis of representative cases, including most previously published SE-related genes in plants. In total, more than half had not been identified previously as SE-related genes, including dominant crucial genes involved in transcription, post-transcription, and transportation, and about one-third had not been reported previously to GenBank or were expected to be unknown, or newly identified genes. We used cDNA arrays to further investigate the expression patterns of these genes in differentiating gradient culture, ranging from pro-embryogenic masses to somatic embryos at every stage. The cDNA collection is composed of a broad repertoire of SE genes which is an important resource for understanding the genetic interactions underlying SE signaling and regulation. Our results suggested that a complicated and concerted mechanism involving multiple cellular pathways is responsible for cotton SE. This report represents a systematic and comprehensive analysis of genes involved in the process of somatic embryogenesis.

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Section: Several Major Types Of Signaling Molecules Are Involved In Sementioning

confidence: 91%

Section: Introductionmentioning

confidence: 97%

Isolation and Characterization of Genes Associated to Cotton Somatic Embryogenesis by Suppression Subtractive Hybridization and Macroarray*

et al. 2006

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“…Analysis of proteome and transcriptome has led to the identification and characterization of certain genes involved in somatic embryogenesis (Lin et al 1996;Giroux and Pauls 1997;Sallandrouze et al 1999;ThibaudNissen et al 2003;Stasolla et al 2004;Imin et al 2005;Rensing et al 2005;Rani et al 2005;Ben et al 2005;Zeng et al 2006;Che et al 2006;Winkelman et al 2006). However, relatively little is known about the early inductive events underlying the transition of the somatic cell into an embryonic pattern of differentiation.…”

Section: Introductionmentioning

confidence: 96%

Analysis of expression profile of selected genes expressed during auxin-induced somatic embryogenesis in leaf base system of wheat (Triticum aestivum) and their possible interactions

et al. 2007

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Somatic embryogenesis is a notable illustration of plant totipotency and involves reprogramming of development in somatic cells toward the embryogenic pathway. Auxins are key components as their exogenous application recuperates the embryogenic potential of the mitotically quiescent somatic cells. In order to unravel the molecular basis of somatic embryogenesis, cDNA library was made from the regeneration proficient wheat leaf base segments treated with auxin. In total, 1440 clones were sequenced and among these 1,196 good quality sequences were assembled into 270 contigs and 425 were singletons. By reverse northern analysis, a total of 57 clones were found to be upregulated during somatic embryogenesis, 64 during 2,4-D treatment, and 170 were common to 2,4-D treatment and somatic embryogenesis. A substantial number of genes involved in hormone response, signal transduction cascades, defense, anti-oxidation, programmed cell death/senescence and cell division were identified and characterized partially. Analysis of data of select genes suggests that the induction phase of somatic embryogenesis is accompanied by the expression of genes that may also be involved in zygotic embryogenesis. The developmental reprogramming process may in fact involve multiple cellular pathways and unfolding of as yet unknown molecular events. Thus, an interaction network draft using bioinformatics and system biology strategy was constructed. The outcome of a systematic and comprehensive analysis of somatic embryogenesis associated interactome in a monocot leaf base system is presented.

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“…The factors controlling this process have been studied extensively in many plant species (Satoh et al, 1986;Coutos-Thevenot et al, 1992, 1993Nielsen and Hansen, 1992;Hendriks and de Vries, 1995;Giroux and Pauls, 1996;Poulsen et al, 1996;Tsukahara and Komamine, 1997). Generally, this phenomenon requires two main steps, including first, the induction of the embryogenic potential by exogenous auxin, allowing the formation of embryogenic cells, and second, the cultures should be transferred to auxin-free medium, which leads to the development of somatic embryos through characteristic stages (Coutos-Thevenot et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Proteins related with embryogenic potential in callus and cell suspensions of sugarcane (Saccharum sp.)

Oropeza

Marcano

García

2001

In Vitro Cell.Dev.Biol.-Plant

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Previous results have shown that some proteins secreted in the culture medium are involved with the formation of embryogenic cells and can modify somatic embryo differentiation. Undifferentiated cell suspensions grown in the presence of 13 mM 2,4-dichlorophenoxyacetic acid (2,4-D) and obtained from embryogenic and non-embryogenic callus were used to study these events in sugarcane plants (cv. PR-62258). The cell suspension growth curves were determined and soluble proteins were extracted from embryogenic and non-embryogenic callus and culture medium from cell suspensions. In embryogenic callus we detected 1.43 times more protein than in non-embryogenic callus and the electrophoretic protein patterns show specific polypeptides for both callus types. In embryogenic callus we detected a cluster of four polypeptides in the range of 38±44 kDa and another polypeptide of 23 kDa that were not observed in non-embryogenic callus. In nonembryogenic callus there is a 35-kDa polypeptide that was not detected in embryogenic callus. In the case of extracellular proteins, the medium from embryogenic cell suspensions contained four polypeptides of 41, 38, 34 and 28 kDa that were slightly detected in the medium from non-embryogenic cell cultures; we also detected a band at 15 kDa that could not be observed in the medium from non-embryogenic cell suspensions. These results suggest that the development of embryogenic callus and cell suspensions is related to the type and amount of intracellular proteins in the callus cells and to the secreted proteins from these cells into the medium.

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Characterization of embryogenesis-related proteins in alfalfa (Medicago sativa)

Cited by 9 publications

References 18 publications

Isolation and Characterization of Genes Associated to Cotton Somatic Embryogenesis by Suppression Subtractive Hybridization and Macroarray*

Isolation and Characterization of Genes Associated to Cotton Somatic Embryogenesis by Suppression Subtractive Hybridization and Macroarray*

Analysis of expression profile of selected genes expressed during auxin-induced somatic embryogenesis in leaf base system of wheat (Triticum aestivum) and their possible interactions

Proteins related with embryogenic potential in callus and cell suspensions of sugarcane (Saccharum sp.)

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