Characterization of embryogenesis-related proteins in alfalfa (Medicago sativa). -Physiol, Plant, 96: 585-592.Large samples of embryogenic ceils can be obtained from tetraploid alfalfa (Medicago sativa L,) suspension cultures following induction by auxin and culture on growth regulator-free medium, A one-dimensional sodium dodecyl sulphate-polyacr}.'lamide gel electrophoresis (SDS-PAGE) comparison of the polypepiides synthesized by embryogenic and nonembryogenic alfalfa genotypes during inductive conditions identified a 50-kDa polypeptide band that was specific to embryogenic genotypes. Two-dimensional (iso electric focusing SDS-PAGE) electrophoretic separations resolved the 50-kDa baad into three 50-kDa polypeptides with different iso-electric points, A comparison of the polypeptides in the soluble and microsomal membrane fractions from embrj'ogenic cell masses of an embryogenic genotype suggested that the 50-kDa peptides are membrane associated. The in vitro translation products from RNAs of an embryogenic genotype had a 5Q-kDa band that was absent from the in vitro hanslation products obtained from RNAs of a nonembryogenic genotype. Therefore, we concluded thai the membrane-localized 50-kDa proteins are involved in somatic embryogenesis in alfalfa and that their expression is transcriptionally determined.
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