Proteins related with embryogenic potential in callus and cell suspensions of sugarcane (Saccharum sp.)

Abstract

Previous results have shown that some proteins secreted in the culture medium are involved with the formation of embryogenic cells and can modify somatic embryo differentiation. Undifferentiated cell suspensions grown in the presence of 13 mM 2,4-dichlorophenoxyacetic acid (2,4-D) and obtained from embryogenic and non-embryogenic callus were used to study these events in sugarcane plants (cv. PR-62258). The cell suspension growth curves were determined and soluble proteins were extracted from embryogenic and non-embryogenic callus and culture medium from cell suspensions. In embryogenic callus we detected 1.43 times more protein than in non-embryogenic callus and the electrophoretic protein patterns show specific polypeptides for both callus types. In embryogenic callus we detected a cluster of four polypeptides in the range of 38±44 kDa and another polypeptide of 23 kDa that were not observed in non-embryogenic callus. In nonembryogenic callus there is a 35-kDa polypeptide that was not detected in embryogenic callus. In the case of extracellular proteins, the medium from embryogenic cell suspensions contained four polypeptides of 41, 38, 34 and 28 kDa that were slightly detected in the medium from non-embryogenic cell cultures; we also detected a band at 15 kDa that could not be observed in the medium from non-embryogenic cell suspensions. These results suggest that the development of embryogenic callus and cell suspensions is related to the type and amount of intracellular proteins in the callus cells and to the secreted proteins from these cells into the medium.

Induction of somatic embryogenesis and biochemical characterization of Cordyline australis (G. Forst.) Endl. ‘Red Star’ callus

“…3d). Our result is similar to the observations of Oropeza et al (2001) and Nieves et al (2008) who detected more protein in embryogenic callus of Saccharum sp. in comparison to callus of non-embryogenic origin.…”

Genotypic variation and aging effects on the embryogenic capability of Kalopanax septemlobus

“…It was found that the 2.5-year-old callus of the KP-G genotype was composed of vacuolated cells devoid of cytoplasmic content, whereas the 6-month-old callus of the same genotype consisted of cells with dense cytoplasmic contents. Oropeza et al (2001) detected that, in sugarcane, the embryogenic callus contained densely cytoplasmic dividing cells with prominent nuclei, whereas the non-embryogenic callus contained vacuolated cells with sparse cytoplasm and few starch grains. They concluded that the embryogenic cells had a high metabolic activity with higher levels of proteins, mRNAs and other cytoplasmic components, whereas nonembryogenic cells had a low metabolic activity.…”

Somatic embryogenesis in Solanum tuberosum from cell suspension cultures: histological analysis and extracellular protein patterns

“…Few proteins associated with the somatic embryogenesis process have been identified and protein patterns were relatively stable during the culture (Hahne et al,1988;Ramagopal, 1989;Boyer et al, 1993;Oropeza et al, 2001). In this research, we found that there are several changes in protein patterns during the development of SEs.…”