Proteins related with embryogenic potential in callus and cell suspensions of sugarcane (Saccharum sp.)

Abstract

Previous results have shown that some proteins secreted in the culture medium are involved with the formation of embryogenic cells and can modify somatic embryo differentiation. Undifferentiated cell suspensions grown in the presence of 13 mM 2,4-dichlorophenoxyacetic acid (2,4-D) and obtained from embryogenic and non-embryogenic callus were used to study these events in sugarcane plants (cv. PR-62258). The cell suspension growth curves were determined and soluble proteins were extracted from embryogenic and non-embryogenic callus and culture medium from cell suspensions. In embryogenic callus we detected 1.43 times more protein than in non-embryogenic callus and the electrophoretic protein patterns show specific polypeptides for both callus types. In embryogenic callus we detected a cluster of four polypeptides in the range of 38±44 kDa and another polypeptide of 23 kDa that were not observed in non-embryogenic callus. In nonembryogenic callus there is a 35-kDa polypeptide that was not detected in embryogenic callus. In the case of extracellular proteins, the medium from embryogenic cell suspensions contained four polypeptides of 41, 38, 34 and 28 kDa that were slightly detected in the medium from non-embryogenic cell cultures; we also detected a band at 15 kDa that could not be observed in the medium from non-embryogenic cell suspensions. These results suggest that the development of embryogenic callus and cell suspensions is related to the type and amount of intracellular proteins in the callus cells and to the secreted proteins from these cells into the medium.

Induction of somatic embryogenesis and biochemical characterization of Cordyline australis (G. Forst.) Endl. ‘Red Star’ callus

“…3d). Our result is similar to the observations of Oropeza et al (2001) and Nieves et al (2008) who detected more protein in embryogenic callus of Saccharum sp. in comparison to callus of non-embryogenic origin.…”

Induction of somatic embryogenesis and biochemical characterization of Cordyline australis (G. Forst.) Endl. ‘Red Star’ callus

“…3d). Our result is similar to the observations of Oropeza et al (2001) and Nieves et al (2008) who detected more protein in embryogenic callus of Saccharum sp. in comparison to callus of non-embryogenic origin.…”

Somatic embryogenesis in Solanum tuberosum from cell suspension cultures: histological analysis and extracellular protein patterns

“…Few proteins associated with the somatic embryogenesis process have been identified and protein patterns were relatively stable during the culture (Hahne et al,1988;Ramagopal, 1989;Boyer et al, 1993;Oropeza et al, 2001). In this research, we found that there are several changes in protein patterns during the development of SEs.…”

Molecular analysis of somatic embryogenesis through proteomic approach and optimization of protocol in recalcitrant Musa spp.

“…Proteomics is the most powerful approach to understand the molecular mechanisms of SE as proteins are directly involved in the functions and cellular biochemical processes and phenotypic characters of cells during growth and development (Pan et al , Xu et al , Ahmad et al ). This has been well documented in many commercial crops (Oropeza et al , Baba et al , Hammami et al , Inocente et al , Tonietto et al , Vale et al , Liu et al ). The proteins indentified through proteomic analysis can be used to improve SE in recalcitrant plant species by modifying the media composition and in vitro growth conditions (Marsoni et al ).…”

Induction of somatic embryogenesis and biochemical characterization of Cordyline australis (G. Forst.) Endl. ‘Red Star’ callus

“…3d). Our result is similar to the observations of Oropeza et al (2001) and Nieves et al (2008) who detected more protein in embryogenic callus of Saccharum sp. in comparison to callus of non-embryogenic origin.…”

Somatic embryogenesis in Solanum tuberosum from cell suspension cultures: histological analysis and extracellular protein patterns

“…Few proteins associated with the somatic embryogenesis process have been identified and protein patterns were relatively stable during the culture (Hahne et al,1988;Ramagopal, 1989;Boyer et al, 1993;Oropeza et al, 2001). In this research, we found that there are several changes in protein patterns during the development of SEs.…”

Molecular analysis of somatic embryogenesis through proteomic approach and optimization of protocol in recalcitrant Musa spp.

“…Proteomics is the most powerful approach to understand the molecular mechanisms of SE as proteins are directly involved in the functions and cellular biochemical processes and phenotypic characters of cells during growth and development (Pan et al , Xu et al , Ahmad et al ). This has been well documented in many commercial crops (Oropeza et al , Baba et al , Hammami et al , Inocente et al , Tonietto et al , Vale et al , Liu et al ). The proteins indentified through proteomic analysis can be used to improve SE in recalcitrant plant species by modifying the media composition and in vitro growth conditions (Marsoni et al ).…”

Induction of somatic embryogenesis and biochemical characterization of Cordyline australis (G. Forst.) Endl. ‘Red Star’ callus

“…3d). Our result is similar to the observations of Oropeza et al (2001) and Nieves et al (2008) who detected more protein in embryogenic callus of Saccharum sp. in comparison to callus of non-embryogenic origin.…”

Somatic embryogenesis in Solanum tuberosum from cell suspension cultures: histological analysis and extracellular protein patterns

“…Few proteins associated with the somatic embryogenesis process have been identified and protein patterns were relatively stable during the culture (Hahne et al,1988;Ramagopal, 1989;Boyer et al, 1993;Oropeza et al, 2001). In this research, we found that there are several changes in protein patterns during the development of SEs.…”

Molecular analysis of somatic embryogenesis through proteomic approach and optimization of protocol in recalcitrant Musa spp.

“…Proteomics is the most powerful approach to understand the molecular mechanisms of SE as proteins are directly involved in the functions and cellular biochemical processes and phenotypic characters of cells during growth and development (Pan et al , Xu et al , Ahmad et al ). This has been well documented in many commercial crops (Oropeza et al , Baba et al , Hammami et al , Inocente et al , Tonietto et al , Vale et al , Liu et al ). The proteins indentified through proteomic analysis can be used to improve SE in recalcitrant plant species by modifying the media composition and in vitro growth conditions (Marsoni et al ).…”