Characterization of dental caries by LIF spectroscopy with 404-nm excitation

Thomas, Shiny Sara; Jayanthi, J.L.; Subhash, Narayanan; Thomas, Joji; Mallia, Rupananda J.; Aparna, Geetha Nair

doi:10.1007/s10103-010-0771-3

Cited by 11 publications

(10 citation statements)

References 15 publications

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“…Variety of bacterial species make it complex to recog nize every contributor, but one point can be confirmed that red fluorescence intensity enhancement could be used to discriminate the carious lesion from the sound tissue. Unlike the presence of obvious peak wavelength in red bands [21,22], the difference in this paper could be explained by that the fluorescence intensity of internal fluorophores overlapped the bacteria contri bution.…”

Section: Discussioncontrasting

confidence: 60%

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Study of validity of autofluorescence-based incipient occlusal caries detection in vitro compared to DIAGNOdent pen

et al. 2012

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The validity of laser induced fluorescence spectroscopy for early diagnosis of occlusal caries irra diated by 403 nm diode laser compared to commercial device DIAGNOdent pen was investigated. The quan titative evaluation parameter based on the fluorescence intensity area ratio of different bands F(440-480 nm)/F(540-700 nm) was built. And the linear correlation analysis between fluorescence result and cor responding DIAGNOdent reading is performed to be r = -0.9085. Moreover, the factors of irradiation laser power and excited site influencing the fluorescence distribution was experimentally investigated. The result shows that the fluorescence spectra at different site of sound occlusal surface is different and the higher laser power will lower the sensitivity of evaluation parameter based on the red fluorescence region increasing. The work is of great significance for practical application of laser induced fluorescence at 403 nm.

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Section: Discussioncontrasting

confidence: 60%

“…It is reported the natural carious lesion can be distinguished from sound tissue by the area of the fluorescence bands at 455 and 500 nm under the excited wavelength at 405 nm [20]. The investigation of fluorescence at the excited wavelength of 404 nm with laser tissue interaction power of 1 mW is recently performed [21,22].…”

Section: Introductionmentioning

confidence: 98%

Study of validity of autofluorescence-based incipient occlusal caries detection in vitro compared to DIAGNOdent pen

et al. 2012

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“…3 ). The red fluorescence region around 600 nm was found to be caused by the presence of a porphyrin compound, particularly protoporphyrin IX, in bacteria [Walsh and Shakibaie, 2007;Thomas et al, 2011]. The protoporphyrin IX concentration is reported to be high in Gram-negative oral bacteria, which increase as the dental plaque biofilm becomes more mature.…”

Section: Discussionmentioning

confidence: 99%

Discrimination of Dental Caries Using Colorimetric Characteristics of Fluorescence Spectrum

Chen

Zhu²,

Xu³

et al. 2015

Caries Res

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The feasibility of colorimetric parameters for the discrimination of the stages of dental caries based on a light-induced autofluorescence spectrum at a 405-nm excitation wavelength was investigated. The fluorescence spectra of 4 groups of tooth samples (10 sound, 10 early-stage decay, 14 established decay, and 10 severe decay), which were classified by the International Caries Detection and Assessment System, were experimentally measured in vitro. The carious lesion samples had an additional fluorescence peak at around 627 nm. The mathematical relation of the fluorescence spectrum and human color perception was established and computed. With increasing severity, the fluorescence color changed from green to yellow according to the colorimetric parameters of the CIE 1931 (x, y) chromaticity coordinates and dominant wavelengths. The results from a one-way ANOVA of the dominant wavelength showed a statistically significant difference among the 4 classified groups. The colorimetric parameters of the light-induced fluorescence spectrum can potentially be applied to evaluate the various carious levels.

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“…22,23 In contrast, excitation wavelength around 400 nm was found to be best suited for detection of early enamel demineralization. [24][25][26] Furthermore, quantitative light induced fluorescence (QLF) was used to quantify the enamel demineralization and demineralization depth from a single spectral image. 27,28 The existing studies involved either single point spectroscopic measurements providing very little or no spatial information on the lesion and strong dependence of the collected fluorescence signal on the position and orientation of the probe, or imaging at a single spectral band which can be substantially affected by local spectral artefacts.…”

Section: Introductionmentioning

confidence: 99%

Hyperspectral laser-induced autofluorescence imaging of dental caries

Bürmen

Fidler

Pernuš

et al. 2012

Lasers in Dentistry XVIII

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Dental caries is a disease characterized by demineralization of enamel crystals leading to the penetration of bacteria into the dentine and pulp. Early detection of enamel demineralization resulting in increased enamel porosity, commonly known as white spots, is a difficult diagnostic task. Laser induced autofluorescence was shown to be a useful method for early detection of demineralization. The existing studies involved either a single point spectroscopic measurements or imaging at a single spectral band. In the case of spectroscopic measurements, very little or no spatial information is acquired and the measured autofluorescence signal strongly depends on the position and orientation of the probe. On the other hand, single-band spectral imaging can be substantially affected by local spectral artefacts. Such effects can significantly interfere with automated methods for detection of early caries lesions. In contrast, hyperspectral imaging effectively combines the spatial information of imaging methods with the spectral information of spectroscopic methods providing excellent basis for development of robust and reliable algorithms for automated classification and analysis of hard dental tissues. In this paper, we employ 405 nm laser excitation of natural caries lesions. The fluorescence signal is acquired by a state-of-the-art hyperspectral imaging system consisting of a high-resolution acousto-optic tunable filter (AOTF) and a highly sensitive Scientific CMOS camera in the spectral range from 550 nm to 800 nm. The results are compared to the contrast obtained by near-infrared hyperspectral imaging technique employed in the existing studies on early detection of dental caries.

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Characterization of dental caries by LIF spectroscopy with 404-nm excitation

Cited by 11 publications

References 15 publications

Study of validity of autofluorescence-based incipient occlusal caries detection in vitro compared to DIAGNOdent pen

Study of validity of autofluorescence-based incipient occlusal caries detection in vitro compared to DIAGNOdent pen

Discrimination of Dental Caries Using Colorimetric Characteristics of Fluorescence Spectrum

Hyperspectral laser-induced autofluorescence imaging of dental caries

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