The validity of laser induced fluorescence spectroscopy for early diagnosis of occlusal caries irra diated by 403 nm diode laser compared to commercial device DIAGNOdent pen was investigated. The quan titative evaluation parameter based on the fluorescence intensity area ratio of different bands F(440-480 nm)/F(540-700 nm) was built. And the linear correlation analysis between fluorescence result and cor responding DIAGNOdent reading is performed to be r = -0.9085. Moreover, the factors of irradiation laser power and excited site influencing the fluorescence distribution was experimentally investigated. The result shows that the fluorescence spectra at different site of sound occlusal surface is different and the higher laser power will lower the sensitivity of evaluation parameter based on the red fluorescence region increasing. The work is of great significance for practical application of laser induced fluorescence at 403 nm.

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