Characterization of denitrifying granular sludge with and without the addition of external carbon source

Lew, Beni; Stief, Peter; Beliavski, Michael; Ashkenazi, A.; Svitlica, Olivera; Khan, Abid Ali; Tarre, Sheldon; Beer, Dirk de; Green, Michal

doi:10.1016/j.biortech.2012.08.049

Cited by 38 publications

(10 citation statements)

References 31 publications

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“… 31 , increased their relative abundance after the summer. On the other side, other genera involved in denitrification, such as Denitromonas 32 or Hyphomicrobium 29 , and nitrifiers, such as Nitrospira spp. 33 , decreased their relative abundance in September.…”

Section: Resultsmentioning

confidence: 99%

Summer holidays as break-point in shaping a tannery sludge microbial community around a stable core microbiota

Giordano

Boscaro

Munz

et al. 2016

Sci Rep

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Recently, several investigations focused on the discovery of a bacterial consortium shared among different wastewater treatment plants (WWTPs). Nevertheless, the definition of a core microbiota over time represents the necessary counterpart in order to unravel the dynamics of bacterial communities in these environments. Here we performed a monthly survey on the bacterial community of a consortial industrial plant. Objectives of this study were: (1) to identify a core microbiota constant over time; (2) to evaluate the temporal dynamics of the community during one year. A conspicuous and diversified core microbiota is constituted by operational taxonomic units which are present throughout the year in the plant. Community composition data confirm that the presence and abundance of bacteria in WWTPs is highly consistent at high taxonomic level. Our results indicate however a difference in microbial community structure between two groups of samples, identifying the summer holiday period as the break-point. Changes in the structure of the microbial community occur otherwise gradually, one month after another. Further studies will clarify how the size and diversity of the core microbiota could affect the observed dynamics.

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Section: Resultsmentioning

confidence: 99%

Summer holidays as break-point in shaping a tannery sludge microbial community around a stable core microbiota

Giordano

Boscaro

Munz

et al. 2016

Sci Rep

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“…Interestingly, the identified denitrifying bacteria (affiliated to Denitromonas sp.) in the present study was associated with the formation of particles, whereas aggregation in pure Denitromonas cultures has not been observed (Lew et al, 2012).…”

Section: Methane-related Nitrate/nitrite Reduction Under Environmentamentioning

confidence: 50%

“…Modin et al (2007) and references therein showed that most denitrifying bacteria are capable of consuming oxygen and thereby create anoxic microenvironments, which in turn enables denitrification in oxic waters. The occurrence of particles from week 7 on was accompanied by an increase in methane oxidation and a decrease in nitrate and nitrite concentrations (Figure 4, I and II), indicating a potential relationship between cell aggregation and the interaction between different microorganisms, as shown in denitrifying bioreactors studies (Etchebehere et al, 2003;Modin et al, 2007;Lew et al, 2012). Interestingly, the identified denitrifying bacteria (affiliated to Denitromonas sp.)…”

Section: Methane-related Nitrate/nitrite Reduction Under Environmentamentioning

confidence: 98%

A Bioreactor Approach to Investigate the Linkage between Methane Oxidation and Nitrate/Nitrite Reduction in the Pelagic Oxic-Anoxic Transition Zone of the Central Baltic Sea

et al. 2016

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Evidence of aerobic methane oxidation coupled to denitrification has been provided for different freshwater environments, whereas the significance of this process for the marine realm has not been adequately investigated. The goal of this study was to investigate the methane-related reduction of nitrate/nitrite in a marine environment (salinity 8.5). A water sample was collected from the oxic-anoxic transition zone of the Gotland Deep (central Baltic Sea) and the microorganisms contained therein were cultivated in a bioreactor under hypoxic conditions (0.5 µM O 2 ). To enrich the microorganisms involved in the coupled process the bioreactor was continuously sparged with methane as the sole energy and carbon source and simultaneously supplied with a nutrient solution rich in nitrate and nitrite. The bioreactor experiment showed a relationship between the turnover of methane and the concomitant concentration decrease of nitrite and nitrate at the early stage of the experiment. This relationship indicates the role of methanotrophs, which may support heterotrophic denitrifiers by the release of organic compounds as an energy source. Besides, a mixture of uncultured microorganisms, aerobic methanotrophic and heterotrophic denitrifying bacteria were identified in the enrichment culture. Microbial incorporation of nitrite and methane was proven on the cellular and gene levels via 15 NO − / 13 CH 4 incubation experiments and subsequent analyses with nano secondary 2 ion mass spectrometry (NanoSIMS) and stable isotope probing (SIP). The NanoSIMS showed the incorporation of 15 N in almost all the bacteria and in 9% of those there was a concomitant enrichment in 13 C. The relatively low abundance of methane-consuming bacteria in the bioreactor was further reflected in specific fatty acids indicative for type I methanotrophic bacteria. Based on pmoA gene analyses, this bacterium is different from the one that was identified as the only key player of methane oxidation in previous studies in the Gotland Deep, indicating the existence of other subordinate methanotrophic Jakobs et al.Methane-Related Reduction of Nitrate/Nitrite bacteria at that site. The results provide the first indications for the predisposition of a methane-related reduction of nitrate/nitrite under hypoxic conditions in the marine realm, supporting the assumption of an interaction between methanotrophic and denitrifying bacteria which hitherto has only been described for fresh water environments.

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“…Dominant genera were family Phycisphaeracea (9.7%), genus Arcobacter (8.9%), Roseovarius (4.5%), Leucothrix mucor (2.6%), and Bacillus (2.1%). Among them, genus Denitromonas is important in the settling of sludge in marine wastewater with pronounced reduction of nitrate [32,33]. Finally, the bacterial community analyses revealed that the predominant genera in eco-HEMS were different between winter and summer, but the some genera in the marine sediment had mainly adapted to the marine wastewater for efficient removal of N and P. Thus, each predominant genus in summer or winter could be an important influence on the treatment efficiency and settlement.…”

Section: Plos Onementioning

confidence: 99%

Pilot plant study on nitrogen and phosphorus removal in marine wastewater by marine sediment with sequencing batch reactor

Kang

Kim

et al. 2020

PLoS ONE

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Effective biological treatment of marine wastewater is not well-known. Accumulation of nitrogen and phosphorus from land-based effluent is a crucial cause of red-tide in marine systems. The purpose of the study is to reduce nitrogen and phosphorus in marine wastewater with a pilot plant-scale sequencing batch reactor (SBR) system by using marine sediment as eco-friendly and effective biological materials, and elucidate which bacterial strains in sludge from marine sediment influence the performance of SBR. By applying eco-friendly high efficiency marine sludge (eco-HEMS), the treatment performance was 15 m 3 d -1 of treatment amount in 4.5 m 3 of the reactor with the average removal efficiency of 89.3% for total nitrogen and 94.9% for total phosphorus at the optimal operation condition in summer. Moreover, the average removal efficiency was 84.0% for total nitrogen and 88.3% for total phosphorus in winter although biological treatment efficiency in winter is generally lower due to bacterial lower activity. These results were revealed by the DNA barcoding analysis of 16s rRNA amplicon sequencing of samples from the sludge in winter. The comparative analysis of the bacterial community composition in sludge at the high efficiency of the system showed the predominant genera Psychromonas (significantly increased to 45.6% relative abundance), Vibrio (13.3%), Gaetbulibacter (5.7%), and Psychroserpens (4.3%) in the 4 week adaptation after adding marine sediment, suggesting that those predominant bacteria influenced the treatment performance in winter.

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Characterization of denitrifying granular sludge with and without the addition of external carbon source

Cited by 38 publications

References 31 publications

Summer holidays as break-point in shaping a tannery sludge microbial community around a stable core microbiota

Summer holidays as break-point in shaping a tannery sludge microbial community around a stable core microbiota

A Bioreactor Approach to Investigate the Linkage between Methane Oxidation and Nitrate/Nitrite Reduction in the Pelagic Oxic-Anoxic Transition Zone of the Central Baltic Sea

Pilot plant study on nitrogen and phosphorus removal in marine wastewater by marine sediment with sequencing batch reactor

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