Characterization of dehydromonacolin-MV2 from Monascus purpureus mutant

Appl Biochem Biotechnol

Gaonkar

et al. 2010

Atherosclerosis is a chronic inflammatory disease of multiple etiologies. It is associated with the accumulation of oxidized lipids in arterial lesions leading to coronary heart disease. 3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (commonly known as statins) are widely used in cardiovascular disease prevention to lower the cholesterol. The antioxidant activity of HMG-CoA reductase inhibitors was studied by lipid peroxidation inhibition assay, DPPH, and hydroxyl radical scavenging-activity methods. The lovastatin (93%) and simvastatin (96%) showed significant action of lipid peroxidation inhibition compared to other HMG-CoA reductase inhibitors. The DPPH radical and hydroxyl radical scavenging activity of simvastatin was 38% and 33%, respectively. The oxidative modification of serum lipid due to reactive oxygen species causes atherosclerosis. This study revealed the importance of lovastatin and simvastatin to prevent oxidative stress-related cardiovascular diseases.

Section: Inhibition Of Lipid Peroxidationmentioning

confidence: 99%

Statins: 3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) Reductase Inhibitors Demonstrate Anti-Atherosclerotic Character due to Their Antioxidant Capacity

Puttananjaiah

Appl Biochem Biotechnol

Gaonkar

et al. 2010

“…The pharmaceutical applications of polyketides (azaphilones) and monacolins produced by Monascus were evidenced by their antioxidant, antibacterial, anti‐inflammatory properties and ability to reduce body cholesterol (Akihisa et al . 2005; Dhale et al . 2007a,b).…”

Section: Introductionmentioning

confidence: 99%

Production of Monascus Purpureus Pigments; Influenced by Amidase and Acid Protease Activity

Journal of Food Biochemistry

Puttananjaiah

Umesh‐Kumar

et al. 2010

Monascus purpureus grown on rice secretes several polyketide pigments used for coloring foods. The amidase enzymes, L-asparaginase and L-glutaminase activities were identified by rapid plate assay. The pink zone formed around colonies of M. purpureus (Microbial Type Culture Collection [MTCC] 410), hyper pigment and albino were 2.0, 2.4 and 1.2 cm, respectively. The L-asparaginase (0.103 IU) and L-glutaminase (0.139 IU) activity was comparatively more in 170 Units) was observed in CFR 410-22. The mutant CFR 410-11 has secreted more red pigment cultured on rice (2.248 optical density [OD] units) and in broth (0.841 OD units). Significant difference in amidase and acid protease activities of have revealed the importance of these enzymes in pigment production. PRACTICAL APPLICATIONThe isolated albino mutant has overproduced acid protease enzyme. This enzyme finds applications in debittering of protein hydrolysate, cheese ripening and preparation of amino acid mixture from protein. The amidase enzymes produced by Monascus sp. may play a major role in tumor inhibition. 1232 M.A. DHALE ET AL.

“…Dimerumic acid, dihydromonacolin‐MV and dehydromonacolin‐MV2 isolated from Monascus sp. have been characterized for their antioxidant action . Our earlier study on toxicity of RMR did not show any adverse effect on rats and significantly reduced the cholesterol and triacylglycerol levels in serum and liver .…”

Section: Introductionmentioning

confidence: 89%

Antioxidant effect of red mould rice in hypercholesterolemic Wistar male rats

Mohan-Kumari

Cell Biochemistry & Function

Naidu

et al. 2011

Self Cite

The effect of Monascus purpureus red mould rice (RMR) on modulation of lipid metabolism and oxidative stress was studied in hypercholesterolemic rats. Cholesterol feeding for 14 weeks caused a significant increase in the lipid peroxides and total thiols and antioxidant enzymes, viz. glutathione peroxidase (GPx), glutathione reductase (GRd), superoxide dismutase (SOD) and catalase (CAT) in serum and liver in comparison to the control group. However, supplementation of RMR to hypercholesterolemic rats at 8, 12 and 16% significantly increased the GRd, GPx, SOD and CAT activities in serum and liver tissues. Furthermore, RMR feeding significantly decreased total thiols and lipid peroxides and also increased other antioxidant molecules such as glutathione and ascorbic acid in high-cholesterol fed rats. The efficiency of RMR (16%) in modulating the antioxidant molecules and antioxidant enzymes is comparable to standard drug-lovastatin. Thus, this study suggests that the long-term administration of RMR may play an important role in suppressing oxidative stress and, thus, may be useful for the prevention and/or early treatment of hypercholesterolemia.