Production of Monascus Purpureus Pigments; Influenced by Amidase and Acid Protease Activity

Abstract

Monascus purpureus grown on rice secretes several polyketide pigments used for coloring foods. The amidase enzymes, L-asparaginase and L-glutaminase activities were identified by rapid plate assay. The pink zone formed around colonies of M. purpureus (Microbial Type Culture Collection [MTCC] 410), hyper pigment and albino were 2.0, 2.4 and 1.2 cm, respectively. The L-asparaginase (0.103 IU) and L-glutaminase (0.139 IU) activity was comparatively more in 170 Units) was observed in CFR 410-22. The mutant CFR 410-11 has secreted more red pigment cultured on rice (2.248 optical density [OD] units) and in broth (0.841 OD units). Significant difference in amidase and acid protease activities of have revealed the importance of these enzymes in pigment production. PRACTICAL APPLICATIONThe isolated albino mutant has overproduced acid protease enzyme. This enzyme finds applications in debittering of protein hydrolysate, cheese ripening and preparation of amino acid mixture from protein. The amidase enzymes produced by Monascus sp. may play a major role in tumor inhibition. 1232 M.A. DHALE ET AL.

Effects of koji‐making with mixed strains on physicochemical and sensory properties of Chinese‐type soy sauce

“…Similar phenomena described earlier were also observed in red mold rice‐incorporated soy sauce . The higher levels of color intensities in SSAOM might be due to the capacity to produce natural pigment of M. purpureus and the Maillard reaction products generated during fermentation …”

“…18 The higher levels of color intensities in SSAOM might be due to the capacity to produce natural pigment of M. purpureus and the Maillard reaction products generated during fermentation. 6,32,33…”

Extracellular biosynthesis of anti‐Candida silver ‎nanoparticles using Monascus purpureus

“…on the rice grains to produce valuable secondary metabolites viz. visual pigments that ranged from bright yellow to deep red in both solid‐state and submerged cultivation . In addition, Monacolin K was investigated as an inhibitor for cholesterol synthesis .…”

A Rapid and Efficient Dye Based Plate Assay Technique for Screening of L-Asparaginase Producing Fungal Strains

“…Enzyme activities were determined by direct nesslerization of NH3 and were expressed as international units (IU) ml −1 [16]. After 96 h of incubation, the release of ammonia was indicated by the increase in the pH of the culture medium.…”