Characterization of Defective Interfering RNA Components That Increase Symptom Severity of Broad Bean Mottle Virus Infections

Romero, Javier; Huang, Qi; Pogany, Judit; Bujarski, Józef J.

doi:10.1006/viro.1993.1297

Cited by 57 publications

(53 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This, in turn, may influence the patterns of selection of BBMV RNA segments. Consistent with the removal of RNA 3-B molecules in bean plants, we reported previously the removal of DI RNAs in BBMVinfected bean plants (Romero et al, 1993). The putative effect of BBMV RNA 3 leader sequences on RNA replication, on the kinetics of translation initiation, or on the interaction with host factors requires experimental confirmation.…”

Section: Iaua Aa Aa01caa0cuc00supporting

confidence: 71%

“…The availability of infectious transcripts has allowed us to initiate BBMV infections from a homogeneous population of genomic RNA molecules. This will be useful for investigation of the mechanisms involved in generation of heterogeneous RNA 3 leader sequences and in the formation of DI RNAs in BBMV (Romero et al, 1993).…”

Section: Iaua Aa Aa01caa0cuc00mentioning

confidence: 99%

“…Further experiments demonstrated that this particular RNA preparation of the Ba strain contained a defective interfering (DI) RNA component that encoded a truncated 2a protein (J. Pogany & J. Bujarski, unpublished results;Romero et aL, 1993).…”

mentioning

confidence: 99%

“…The first-strand cDNA was synthesized with Moloney murine leukaemia virus reverse transciptase as described by Romero et al (1993). The amplification of double-stranded cDNA was accomplished by 25 PCR thermocycles (92°C, 1 rain; 57 °C, 2 rain; 72 °C, 2.5 min) in a reaction mixture as described by Romero et al (1993), using Vent DNA polymerase (New England Biolabs). One difficulty in designing the correct transcription initiation sites was that BBMV RNAs initiated with an A residue.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Infectious transcripts from PCR-amplified broad bean mottle bromovirus cDNA clones and variable nature of leader regions in RNA 3 segment

Pogany

Huang

Romero

et al. 1994

Journal of General Virology

View full text Add to dashboard Cite

show abstract

Section: Iaua Aa Aa01caa0cuc00supporting

confidence: 71%

Section: Iaua Aa Aa01caa0cuc00mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Infectious transcripts from PCR-amplified broad bean mottle bromovirus cDNA clones and variable nature of leader regions in RNA 3 segment

Pogany

Huang

Romero

et al. 1994

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…It is important to analyse the relationships that exist among these molecules, the helper virus, the host and the effect of environmental conditions on their generation. Many D-RNAs have been well characterized for most animal RNA virus groups (Holland, 1990) and several plant RNA virus groups including tombusvirus (Hillman et al, 1987), carmovirus (Li et al, 1989), potexvirus (White et al, 1991), closterovirus (Mawassi et al, 1995;Rubio et al, 2000), tospovirus (Inoue-Nagata et al, 1998), tobamovirus (Lewandoski and Dawson, 1998), cucumovirus (Graves and Roosinck, 1995 and bromovirus (Romero et al, 1993;Pogany et al, 1995). All of them are derived from the helper virus genome by simple internal deletions or by complex rearrangements of genomic sequences (Simon and Bujarski, 1994).…”

mentioning

confidence: 99%

Analysis of external factors that affect formation and accumulation of D-RNAs in Bromovirus infections

Sandoval

Llamas

Romero³

2008

Span J Agric Res

View full text Add to dashboard Cite

Defective RNA molecules (D-RNAs) are being studied in several plant RNA virus groups. In the genus Bromovirus, D-RNAs have been described for Broad bean mottle virus (BBMV), they are formed by a single internal deletion in the RNA2 and have been generated de novo by serial passages. In contrast, in Brome mosaic virus (BMV) D-RNAs are generated by a single or double internal deletion in the RNA3 without serial passages in their hosts. In this work the external effects of the host and the growth temperature in the generation and accumulation of D-RNAs in BBMV, BMV and Cowpea chlorotic mottle virus (CCMV) are studied. The BBMV and BMV D-RNAs were generated and accumulated with or without serial passages of the viruses in different hosts and cultivars. Plants grown at 12, 16, 20 and 24°C in a growth chamber and in a greenhouse (22 ± 5°C) generated D-RNAs after being inoculated with virusfree D-RNAs, with and without passages. The D-RNAs observed in BBMV and BMV presented some common characteristics: both were formed de novo after serial passages or without passages, their deletion borders had short repeated and palindrome sequences that favour recombination. In addition, growing inoculated plants at lower temperatures greatly facilitated the generation and accumulation of D-RNAs. The CCMV did not generate defective molecules in cowpea (Vigna unguiculata) and Nicotiana benthamiana plants after several serial passages or without passages. This is the first time that D-RNAs have been generated in BBMV without passages and BMV with serial passages.Additional key words: BBMV, BMV, CCMV, host effects, temperature conditions. Resumen Análisis de los factores externos que afectan a la formación y acumulación de los D-RNAs en infecciones de BromovirusLos RNAs defectivos (D-RNAs) están siendo estudiados en diversos virus RNA de plantas. En el género Bromovirus se han descrito D-RNAs en el virus del moteado del haba (BBMV), formados mediante una delección interna en el RNA2, que se generan de novo mediante pases seriados del virus sobre un nuevo huésped. En contraste, en el virus del mosaico del bromo (BMV), los D-RNAs se forman por una o dos delecciones en el RNA3 sin necesidad de pases en sus huéspedes. En este trabajo estudiamos algunos factores externos, como los huéspedes, las inoculaciones y las temperaturas de crecimiento en la generación y acumulación de D-RNAs en BBMV, BMV y en el virus del moteado clorótico de la carilla (CCMV). Los D-RNAs de BBMV y BMV fueron generados y acumulados mediante pases seriados y sin pases de los virus en diferentes huéspedes y cultivares. Plantas inoculadas con BBMV y BMV sin D-RNAs, mantenidas a 12, 16, 20 y 24°C en cámaras de crecimiento a temperatura constante o en un invernadero (22 ± 5°C), generaron D-RNAs con o sin pases sobre nuevos huéspedes. Los D-RNAs observados en ambos virus presentan algunas características en común: son formados de novo mediante pases seriados o purificados de hojas infectadas algunas semanas después de la inoculación; los bordes de la delección tie...

show abstract

Defective interfering RNAs and defective viruses associated with multipartite RNA viruses of plants

Graves

Pogany

Romero³

1996

Seminars in Virology

View full text Add to dashboard Cite

Characterization of Defective Interfering RNA Components That Increase Symptom Severity of Broad Bean Mottle Virus Infections

Cited by 57 publications

References 0 publications

Infectious transcripts from PCR-amplified broad bean mottle bromovirus cDNA clones and variable nature of leader regions in RNA 3 segment

Infectious transcripts from PCR-amplified broad bean mottle bromovirus cDNA clones and variable nature of leader regions in RNA 3 segment

Analysis of external factors that affect formation and accumulation of D-RNAs in Bromovirus infections

Defective interfering RNAs and defective viruses associated with multipartite RNA viruses of plants

Contact Info

Product

Resources

About