Characterization of conformers and dimers of antithrombin by capillary electrophoresis-quadrupole-time-of-flight mass spectrometry

Marie, Anne-Lise; Domínguez‐Vega, Elena; Saller, François; Plantier, Jean‐Luc; Urbain, Rémi; Borgel, Delphine; Tran, Nguyet Thuy; Somsen, Govert W.; Taverna, Myriam

doi:10.1016/j.aca.2016.10.016

Cited by 21 publications

(29 citation statements)

References 33 publications

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“…They were able to separate two conformers and concluded that a sheathless interface is best suited to maintain conformational integrity after ionization . Another study demonstrated the identification of several conformers of antithrombin based on their different charge‐state‐distributions . Belov et al.…”

Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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Capillary electrophoresis (CE) offers fast and high‐resolution separation of charged analytes from small injection volumes. Coupled to mass spectrometry (MS), it represents a powerful analytical technique providing (exact) mass information and enables molecular characterization based on fragmentation. Although hyphenation of CE and MS is not straightforward, much emphasis has been placed on enabling efficient ionization and user‐friendly coupling. Though several interfaces are now commercially available, research on more efficient and robust interfacing with nano‐electrospray ionization (ESI), matrix‐assisted laser desorption/ionization (MALDI) and inductively coupled plasma mass spectrometry (ICP) continues with considerable results. At the same time, CE‐MS has been used in many fields, predominantly for the analysis of proteins, peptides and metabolites. This review belongs to a series of regularly published articles, summarizing 248 articles covering the time between June 2016 and May 2018. Latest developments on hyphenation of CE with MS as well as instrumental developments such as two‐dimensional separation systems with MS detection are mentioned. Furthermore, applications of various CE‐modes including capillary zone electrophoresis (CZE), nonaqueous capillary electrophoresis (NACE), capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF) coupled to MS in biological, pharmaceutical and environmental research are summarized.

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Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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“…CE allows protein analysis under near-physiological conditions, allowing detection of the different conformers. 149 − 151 For example, the unfolding of wild-type β2-microglobulin under nondenaturing conditions was investigated with CE-UV 149 and CE-ESI-MS. 150 In the latter study, several interfaces and mass spectrometers were compared. With CE-ESI-TOF MS proteoforms differing by 1 Da only could be assigned and sheathless interfacing appeared best suited to preserve protein structure integrity.…”

Section: Applicationsmentioning

confidence: 99%

“…A CE-MS method developed to separate the conformers and dimers of the drug antithrombin employed a neutral poly(vinyl alcohol)-coated capillary. 151 The protein conformation was preserved by using a BGE at physiological pH. The developed method allowed the detection of the native, latent, and heterodimer conformers in formulation.…”

Section: Applicationsmentioning

confidence: 99%

Capillary Electrophoresis: Trends and Recent Advances

et al. 2018

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“…In the case of Aclotine, the MS data between the separated conformers showed dissimilarities concerning the relative abundance of each detected charge state, suggesting that some protonation sites were accessible to the solvent depending on the conformation of the protein (Figure ). That original work is particularly interesting because it illustrates the powerful analytical methodologies achievable at the moment solely using CE/MS …”

Section: Applications and Potential Of Ce/ms For The Analysis Of Biommentioning

confidence: 99%

“…(B) and (C) mass spectra of peaks 1 and 2, respectively. Adapted from Marie et al Copyright (2016) Elsevier BV…”

Section: Applications and Potential Of Ce/ms For The Analysis Of Biommentioning

confidence: 99%

Revealing the potential of capillary electrophoresis/mass spectrometry: the tipping point

Gahoual

Leize‐Wagner

Houzé

et al. 2018

Rapid Comm Mass Spectrometry

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The hyphenation of capillary electrophoresis and mass spectrometry (CE/MS) remains a minor technique compared with liquid chromatography/mass spectrometry (LC/MS), which represents nowadays the standard instrumentation, regardless of its introduction thirty years ago. However, from a theoretical point of view, CE coupling should be quite favorable especially with electrospray ionization mass spectrometry (ESI-MS). At the time, the sensitivity provided by CE/MS was often limited, due to hyphenation requirements, which at some point appeared to disqualify CE/MS from benefiting from the performance gain driving the evolution of MS instruments. However, this context has been significantly modified in a matter of a few years. The development of innovative CE/MS interfacing systems has enabled an important improvement regarding sensitivity and reinforced robustness in order to provide an instrumentation accessible to the largest scientific community. Because of the unique selectivity delivered by the electrophoretic separation, CE/MS has proved to be particularly relevant for the analysis of biological molecules. The conjunction of these aspects is motivating the interest in CE/MS analysis and shows that CE/MS is mature enough to enrich the toolbox of analytical techniques for the analysis of complex biological samples. Here we discuss the characteristics of the major types of high-sensitivity CE/ESI-MS instrumentation and emphasize the late evolution and future positioning of CE/MS analysis for the characterization of biological molecules like peptides and proteins, through some pertinent applications.

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Characterization of conformers and dimers of antithrombin by capillary electrophoresis-quadrupole-time-of-flight mass spectrometry

Cited by 21 publications

References 33 publications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Capillary Electrophoresis: Trends and Recent Advances

Revealing the potential of capillary electrophoresis/mass spectrometry: the tipping point

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