Characterization of complexation of PVP copolymer with DNA

Abstract

Interactions of amphiphilic copolymer poly (N-vinylpyrrolidone-co-N-t-Boc-tryptophanamido-N(-methacrylthiourea) [P(NVP-co-TrpAMT)] and poly(N-vinylpyrrlidone) (PVP) with DNA were studied with the aim to understand the difference in their complexation efficiencies when considered as a potential non-viral delivery vector. Fluorescence spectroscopy, ethidium bromide (EB) quenching, z potential measurement, and agarose gel electrophoresis were all carried out in Tris-HCl (pH 7.4) solution to assess the degree of interaction between the (co)polymers P(NVP-co-TrpAMT) and DNA. All of these measurements show that the high affinity of the copolymer for DNA can be demonstrated. We also found that P(NVP-co-TrpAMT)-I (PVP/PTrpAMT molar ratio was 100:2.88) exhibited a high DNA cleavage activity and induced the supercoiled form of the plasmid cleavage in the nicked and linear forms.Moreover, P(NVP-co-TrpAMT)-I exhibited stronger affinity for DNA than PVP and P(NVP-co-TrpAMT)-II (PVP/ PTrpAMT molar ratio of 100:6.35). This phenomenon is believed to be related to the structure of TrpAMT and the hydrophobic nature of the copolymer. P(NVP-co-TrpAMT)-II with higher hydrophobic segments creates a strong hydrophobic interaction and induces a steric barrier which hinders their complexation with DNA. The study reveals important information about the inner mechanisms involved in the interaction of the PVP copolymer with DNA, which aids in the design of novel efficient non-ionic gene carriers.

Synthesis of polymer nanogels by electro-Fenton process: investigation of the effect of main operation parameters

“…It is used as plasma expander and additive in several pharmaceutical formulations. Emerging PVP-based materials include nanocarriers for gene therapy [46,47] or to enhance the delivery of biomolecules to the brain in the treatment of neurodegenerative diseases [8]. We provided the proof of concept that PVP crosslinking can be induced by an electrochemical process such as EF [44].…”

Biocompatibility of modified polyethersulfone membranes by blending an amphiphilic triblock co-polymer of poly(vinyl pyrrolidone)–b-poly(methyl methacrylate)–b-poly(vinyl pyrrolidone)

“…By directly blending hydrophilic polymers, such as poly-(vinyl pyrrolidone) (PVP) [15], polyethylene glycol (PEG) [16] and poly(acrylic acid-co-vinyl pyrrolidone) (PAA-co-PVP) [17,18], the surface hydrophilicity of the membranes could be increased; the anti-fouling properties and blood compatibility were also improved [19]. PVP is a non-ionic water soluble polymer and exhibits many fascinating properties [20,21]. It was initially used as a blood plasma substitute and later has been applied in a wide variety of applications, such as biomaterials and coatings, contact lenses, disinfectants, intra-ocular lenses, medicine, pharmacy, cosmetics and industrial production [22,23].…”

T3: Targeted proteomics of DNA-binding proteins

“…PVP is biologically inert, nearly chemically neutral, pH stable, and non-ionic making it a suitable environment for reactions to take place without any secondary effects occurring along with producing a suitable surface for MALDI-MS [2; 3; 4]. PVP forms a hydrogel layer capable of absorbing forty times more water than its dry weight and does not dissolve when immersed in most solvents [5].…”