Characterization of circulating CD4+ CD8+ double positive and CD4− CD8− double negative T-lymphocyte in children with β-thalassemia major

Abstract

TRIAL NUMBER: UMIN000018950.

Could the Crosstalk Between Mdscs and Tregs Have a Role in β-thalassemia?

“…Following incubation, red blood cell lysis and washing with PBS were done. Then cells were suspended in PBS and analyzed using Cell Quest software on BD FACSCalibur ow cytometer, as presented in gure (2). An isotype-matched negative control was used for each sample.…”

“…TM is a common health problem in Mediterranean countries, especially Egypt. TM's most common complications are iron overload, heart failure, recurrent infections, and alloimmunization from repeated blood transfusion 2 .…”

“…n number, TLC total leukocyte count, PLT platelet count, HB hemoglobin Data expressed as mean ± SD, * number (percent),Levels of Tregs and MDSCs in patients and healthy controlsA comparison of the levels of Tregs and total MDSCs between BTM patients and controls is shown in table(2). Patients with BTM had higher percentages of both CD4 + CD25 Hi T cells and CD4 + CD25 Hi FoxP3 + Tregs compared to controls.…”

Prognostic value of CD4+CD25+ Tregs as a valuable biomarker for patients with sepsis in ICU

“…[4,5] Activation of Tregs, especially CD4 + CD25 + Tregs via TCR stimulation can suppress the body's excessive inflammatory response through inhibition of activation and proliferation of effect T cells, and of antibody production by B cells. [6,7] Many studies on CD4 + CD25 + Tregs have focused on signal transductions, but few on its application in clinical use. In this study, we investigated the clinical application of CD4 + CD25 + Tregs in peripheral blood as a potential biomarker in evaluating prognosis of patients with sepsis.…”

Research progress of hereditary blood diseases

“…基因的缺失型突变为主, 而β地贫主要为单核苷酸点 突变、插入或小核苷酸序列的插入导致β珠蛋白基因 重要功能区域的移码突变 [1] , 大片段缺失情况并不常 见. 另外, 由于基因突变导致地贫患者蛋白表达及细 胞分化等也存在多种异常, 例如, (ⅰ) 由于贫血导致的 代偿效应, β地贫患者血浆中红系成熟相关miR-451及 miR-155的表达明显上升 [2] ; (ⅱ) 重型、轻型β地贫患 者及正常对照外周血细胞中长片段非编码RNA (long non-coding RNA, lncRNA)及mRNA的表达各不相同, 提示这些差异可能与临床表现相关 [3] ; (ⅲ) 重型β地贫 患者外周血中CD4 + CD8 + , CD4 − CD8 − T及NKT细胞比 例明显增加, 考虑这可能是重型β地贫患者易发生感 染的原因 [4] ; (ⅳ) β地贫患者红细胞发育过程中自噬现 象明显升高 [5] .…”