Characterization of Chlorella sorokiniana, UTEX 1230

Abstract

This paper characterizes the strain Chlorella sorokiniana UTEX 1230 within a laboratory setting using a 1 L bubble column. The findings show that productivity can be trebled under mixotrophic conditions (from 0.2 g·L−1·d−1 to 0.66 g·L−1·d−1) with the addition of sodium acetate. The results also indicate that both the growth rate and final yield increase with the cultivation temperature, with most parameters showing an optimum in the range of 30–35 °C. The maximum specific growth rate was found to be in the region of 0.12 h−1 at a surface irradiance between 100–500 µE·m−2·s−1. This high growth rate makes the strain particularly suited to the rapid production of biomass, suitable for either whole cell bioprocessing or bioremediation. However, the relatively low lipid productivity (9.2 mg·L−1·d−1) confirms previous findings which would indicate poor applicability for biodiesel production. The strain shows greater promise in wastewater treatment applications with removal rates of nitrogen and phosphorus in the region of 37 and 30 mg·L−1·d−1 respectively. Furthermore, the findings show that a fed-batch strategy to inorganic nutrient loading can increase the final yield by around 50% compared to a conventional batch run. This is particularly interesting as fed-batch production techniques are rarely used within microalgal cultivation, so provide an interesting avenue for further investigation. Overall, the findings show that C. sorokiniana UTEX 1230 is a robust and fast-growing microalgal strain suitable both for the laboratory and scale-up.

Effect of iron and magnesium addition on population dynamics and high value product of microalgae grown in anaerobic liquid digestate

“…Moreover, Chlorella sorokiniana was present in the control sample with 3.5% abundance and the dominancy increased more than twice, up to 8.46% when iron was added; and up to 5.26% when magnesium was added. This increase was also reflected on the protein amount of the mixed culture since the dry weight analysis of C. sorokiniana shows that it has 40% protein, 30-38% carbohydrate and 18-22% lipid content 41 . Moreover, bacterial species belonging to Proteobacteria, www.nature.com/scientificreports www.nature.com/scientificreports/ Spirochaetes, Actinobacteria, Bacteroidetes and Gemmatimonadetes phyla had different abundance in control, magnesium and iron added conditions.…”

Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media

“…on BG11 media. C. sorokiniana has the ability to grow fast on culture media with limited nutrients, therefore it can be utilized as a bioremediation agent (Edmundson et al 2017;Lizzul et al 2018).…”

Directed cultivation of Chlorella sorokiniana for the increase in carotenoids’ synthesis

“…Microalgae Chlorella sorokiniana is a perspective producer of valuable components that can be successfully cultivated both industrially and in laboratory conditions [6]. C. sorokiniana's biomass is known for a high level of proteins, carbohydrates, lipids and biologically active substances [7].…”