This article describes the use of Chlorella sorokiniana biomass additives in pasta recipes to enrich the product with biologically active phytonutrients, as well as to achieve the desired color range without the use of synthetic dyes. Samples of dry biomass were obtained by the cultivation of microalgae C. sorokiniana (strain), its quality indicators and nutritional value were determined for use as a food additive. A method of using dry biomass of microalgae C. sorokiniana as a phytoadditive to replace 5% of flour mixture for effective enrichment of pasta with biologically active phytonutrients was proposed. The choice of the optimal amount of addition of microalgae biomass was proved since it turned out that the replacement of flour should be no more than 5% due to the distinct fish flavor of the final product. The present study was conducted to evaluate the effect of adding dry biomass of Chlorella microalgae on total protein, lipid, chlorophyll, and carotenoid content. Substitution of 5% of pasta flour led to an increase in the content of proteins and lipids to 15.7 ± 0.50% and 4.1 ± 0.06%, respectively. Meanwhile, the addition of microalgae Chlorella to pasta has helped to increase the content of polyunsaturated fatty acids, chlorophyll, and carotenoids which are necessary for the prevention of foodborne diseases. The aim of this study is to develop pasta recipe with additives of microalgae biomass C. sorokiniana and study their quality indicators.
We carried out cultivation of Chlorella sorokiniana microalga using various irradiation sources: daylight lamp with spectral range 530-610 nm and filament lamp with spectral range 3.5-5.0 μm. Specific growth rates were evaluated for these conditions at exponential phase. From the obtained biomass we extracted lipids and determined its’ fatty-acid content. Also we analyzed triacylglycerol (TAG) content and unsaturated fatty acids, as well as oleinic acid content. Biomass irradiation by infrared source causes enlarging of total lipid content in alga biomass, as well as saturated fatty acids and oleinic acid portions. It is viable to use infrared irradiation mode for accumulation of non-polar lipids (TAG) at stationary growth phase, which might be used for biofuel obtaining after fractionating. Polar lipid fraction, extracted from biomass at linear growth stage, might be used for obtaining essential polyunsaturated fatty acids.
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