Abstract. Oksari AA, Susanty D, Wanda IF. 2019. Allelopathic effect of invasive species air potato (Dioscorea bulbifera) on seeds germination of Polyalthia littoralis. Nusantara Bioscience 11: 63-70. Dioscorea bulbifera L. (Dioscoreaceae) is included in the Global Weed Compendium and one of the invasive plants that cause environmental problems in many parts of the world. This study aims to determine the effect of allelopathy from various concentrations of leaf and tuber extract from D. bulbifera on seed germination of Polyalthia littoralis (Blume) Boerl. The method used was a Completely Randomized Design (CRD) with seven treatments and three replications. The treatment given was the concentration of D. bulbifera extract 10%, 20%, 40%, 60%, 80%, 100% and aquadest solvent control. The results of phytochemical screening analysis showed that the extract of leaves and tuber of D. bulbifera did not contain alkaloids, but specifically on leaves extract contained tannins. Both extracts contained steroids, flavonoids, and saponins. Based on the results of the study, total phenolic obtained in the leaf extract as much as 28.81 ± 2.51 mg TAE/g extract, while the tuber extract had a total phenolic of 6.74 ± 1.19 mg TAE/g. The results showed that allelopathic substances of D. bulbifera affected the germination of P. littoralis seeds. Based on DMRT test at 5% level, it was known that allelopathy concentration of D. bulbifera significantly affected the germination rate at concentrations of 20%, 60%, 80%, and 100% and significantly affected the coefficient of velocity of germination at a concentration of 20%. But, concentration allelopathy of D. bulbifera had no significant effect on the coefficient of uniformity of germination. The concentration that reduced the lowest seed viability was at a concentration of 80% with an average germination power of 56.67 ± 7.45%.

Program MBKM merupakan langkah awal untuk menyiapkan mahasiswa yang dapat memenuhi tantangan di dunia kerja. Program studi Biologi Universitas Nusa Bangsa telah melakukan program ini, namun perlu evaluasi mengenai implementasi kurikulum MBKM yang telah berjalan. Tujuan dari penelitian ini adalah untuk menganalisis implementasi kurikulum MBKM Program Studi Biologi UNB. Penelitian ini menggunakan metode survey terhadap dosen, mahasiswa, tenaga kependidikan dan mitra. Pengumpulan data menggunakan kuesioner dengan skala Likert. Kuesioner diberikan dengan menggunakan aplikasi Google Form. Kuesioner penelitian sebelumnya telah di uji validitas dan reliabilitasnya. Data yang telah didapat kemudian diolah. Pengolahan data dimulai dari pengkodean (coding), pemasukan data (data entry), pengecekan ulang (cleaning), dan analisis data. Program MBKM nya sudah terlaksana dengan baik. Namun, Prodi Biologi UNB harus tetap melakukan persiapan di setiap kegiatan yang akan berjalan dan evaluasi setelah kegiatan selesai.

FERMENTATION OF ARABIC COFFEE SEEDS (Coffea arabica L.) WITH THE ADDITION OF LACTO ACID BACTERIA (Lactobacillus sp)Coffee bean fermentation is one of a series of processes for making ground coffee that can affect coffee quality. In this study the fermentation of Arabica coffee beans was carried out using lactic acid bacteria (Lactobaciilus sp) in the form of a starter. The analysis was carried out on five samples, namely brand a coffee samples (sample A), coffee samples processed without bacteria (sample B), coffee samples processed with the addition of bacteria and substrate as many as 10:90 (sample C), 20:80 (sample D ), 30:70 (sample E). The five coffee samples were analyzed for water content, pH, extract content, ash content, and caffeine content. Identification of caffeine was carried out by the UV-Vis spectrophotometric method at a wavelength of 275.0397 nm. The highest caffeine content was in sample C at 0.95% and the lowest caffeine content was in sample E 0.71%. The highest extract content was found in sample E at 27.72% and the lowest content in C at 24.60%. The highest water content results were found in sample C of 4.56% and the lowest in sample E of 4.26%. The highest ash content was found in coffee C samples at 4.98% and the lowest ash content in E at 4.43%. The highest value of the degree of acidity (pH) was found in sample B (6.19) and the lowest in sample A (5.41). The results showed that the Arabica ground coffee met the SNI 01-3542-2004 requirements on parameters of moisture content, extract content, ash content and caffeine content. Keywords: Arabica coffee, fermentation, Lactobacillus sp, CaffeineABSTRAKFermentasi biji kopi merupakan salah satu rangkaian proses pengolahan pembuatan kopi bubuk yang dapat mempengaruhi kualitas kopi. Pada penelitian ini fermentasi biji kopi Arabica dilakukan menggunakan bakteri  asam laktat (Lactobaciilus sp) dalam bentuk starter. Analisis dilakukan terhadap lima sampel  yaitu sampel kopi merk a (sampel A), sampel kopi yang diproses tanpa bakteri (sampel B) , sampel kopi yang diproses dengan penambahan bakteri dan substrat sebanyak  10:90 (sampel C), 20:80 (sampel D), 30:70 (sampel E). Kelima sampel kopi tersebut dianalisis kadar air, pH, kadar sari, kadar abu, dan kadar kafein. Identifikasi kafein dilakukan dengan metode spektrofotometri UV-Vis pada panjang gelombang 275,0397 nm. Kadar kafein tertinggi terdapat pada sampel C sebesar 0,95 % dan  kadar kafein terendah pada sampel E 0,71%. Kadar sari tertinggi terdapat pada sampel E sebesar 27,72%  dan kadar sari terendah pada C sebesar 24,60%. Hasil kadar air tertinggi terdapat pada sampel C sebesar 4,56% dan kadar terendah pada sampel E sebesar  4,26%. Kadar abu tertinggi terdapat pada sampel kopi C sebesar 4,98% dan kadar abu terendah pada E sebesar 4,43%. Nilai derajat keasaman (pH) tertinggi didapatkan pada sampel B yaitu sebesar 6,19 dan terendah pada sampel A yaitu 5,41. Hasil uji menunjukkan bahwa kopi bubuk arabika dengan proses fermentasi enggunakan bakteri asam laktat memenuhi syarat SNI 01-3542-2004  pada parameter kadar air, kadar sari, kadar abu dan kadar kafein.Kata kunci: Kopi arabika, fermentasi, Lactobacillus sp, Kafein

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Activated carbon is a solid product resulting from carbonization and activation of lignocellulosic biomass by several methods and activators. Each activator can produce different characteristics of activated carbon. The activated carbon of Oil Palm Empty Fruit Bunches (OPEFB) is processed from Empty Fruit Bunches (EFB) waste, which is quite abundant in Indonesia. The purpose of this study was to determine the characteristics of OPEFB carbon that had been chemically activated using hydrochloric acid (HCl) and phosphoric acid (H3PO4) based on the Indonesian National Standard (SNI) No. 06 - 3730 - 1995. The research method consisted of the process of carbonization of OPEFB using a pyrolysis device at 400oC for 6 hours; activation of carbon with activator HCl and H3PO4 at various activator concentrations; characterization of activated carbon on the parameters of moisture content, ash content, absorption of iodine, and absorption of methylene blue. The results showed that the 0.5000 M HCl was the best activator that produced the quality of OPEFB activated carbon according to SNI 06 - 3730 - 1995 on parameters of moisture content, ash content and absorption of iodine.Keywords: Activated Carbon, Iodine Absorption, Oil Palm Empty Fruit BunchesABSTRAKAktivasi karbon tandan kosong kelapa sawit dengan asam klorida dan asam fosfat serta uji karakterisasi berdasarkan SNI No. 06–3730–1995Karbon aktif merupakan produk padatan yang dihasilkan dari karbonisasi dan aktivasi biomassa lignoselulosa dengan beberapa metode dan aktivator. Masing-masing aktivator dapat menghasilkan karakteristik karbon aktif yang berbeda. Karbon aktif Tandan Kosong Kelapa Sawit (TKKS) diolah dari limbah TKKS yang keberadaannya cukup melimpah di Indonesia. Tujuan penelitian ini adalah mengetahui karakteristik karbon TKKS yang telah diaktivasi secara kimia menggunakan aktivator asam klorida (HCl) dan asam fosfat (H3PO4) berdasarkan Standar Nasional Indonesia  (SNI) No.  06 – 3730 – 1995. Metode penelitian terdiri atas proses karbonisasi TKKS menggunakan alat pirolisis pada suhu 400 ℃ selama 6 jam; aktivasi karbon dengan aktivator HCl dan H3PO4 pada berbagai konsentrasi aktivator; serta karakterisasi karbon aktif pada parameter kadar air dan abu, daya jerap terhadap iod serta daya jerap terhadap biru metilen. Hasil penelitian menunjukkan bahwa HCl 0,5 M merupakan aktivator terbaik dan karbon aktif yang dihasilkan memiliki karakteristik yang sesuai dengan SNI 06 – 3730 – 1995 pada parameter kadar air dan abu serta daya jerap terhadap iod.Kata kunci: Daya Jerap Iod, Karbon Aktif, Tandan Kosong Kelapa Sawit

Untuk menguragi pencemaran lingkungan yang berasal dari limbah ternak ayam broiler, kotoran ayam dapat dimanfaatkan sebagai media tumbuh. Pada penelitian ini, Chlorella sp (inaCC M39) dikultur pada media limbah ternak ayam Broiler dengan konsentrasi 20 gram/L. Limbah yang digunakan merupakan kotoran ayam broiler yang telah kering dan dilakukan uji untuk mengetahui kadar Nitrogen (N), fosfor (P), dan Kalium (K). Uji N menggunakan metode kjhedal, Uji P dan K dengan spektrofotometri. Kadar N pada limbah kotoran ayam yaitu 0,8%, kadar P sebesar 0,041%, dan kadar K sebesar 112,58 mg/L.  Pertumbuhan Chlorella sp diamati selama 16 hari setiap 48 jam dengan spectofotometer pada panjang gelombang 680 nm. Chlorella sp mencapai puncak pertumbuhan pada hari ke-14. Ekstraksi biomassa Chlorella sp dilakukan bertingkat dengan pelarut kloroform dan dilanjutkan dengan pelarut metanol, lalu dilakukan uji fitokimia. Ekstrak metanol Chlorella sp diketahui memiliki kandungan steroid dan saponin.   Kata Kunci : Limbah, Ayam Broiler, Media, Chlorella sp., Ekstrak     ABSTRACT  Extract of Chlorella sp. Cultured in Chicken Livestock Waste Media To reduce environmental pollution from broiler chicken waste, chicken manure can be used as a growing medium. In this study, Chlorella sp. (inaCC M39) was cultured on Broiler chicken waste media with a concentration of  20 grams / L. The waste used was dried broiler chicken manure and tested to determine levels of Nitrogen (N), phosphorus (P), and Potassium (K). The test for N uses the kjhedal method, P and K were tested with spectrophotometry methods. The content of N in chicken manure are 0.8%, P are 0.041%, and K are 112.58 mg / L. Chlorella sp. growth was observed for 16 days every 48 hours with a spectrophotometer at a wavelength of 680 nm. Chlorella sp. reaches peak growth on day 14. Chlorella sp. biomass was extracted with chloroform solvents and followed by methanol solvents, then phytochemical tests were performed. Chlorella sp. methanol extract is known to contain steroids and saponins.   Keywords: Waste, Broiler Chicken, Media, Chlorella sp., Extract

Isolation and Fatty Acid Analysis of Scenedesmus quadricauda from freshwaterMicroalgae have fast growth rate and fatty acids which potential as bioenergy. In addition, microalgae are also living with binding carbon dioxide for photosynthesis, so as to reduce air pollution. In this study, samples were taken from the freshwater, Sawahan, Padang, West Sumatra. Nile red staining performed on the samples to see the potential of lipid content. Microalgae having lipid content were isolated using the capillary pipette technique then it was identified using a microscope. Isolated microalgae was identified as Scenedesmus quadricauda. Microalgae growth measured by optical density value. It cultivated in different concentrations of NaNO3 to see the effect of the amount of nitrogen on the growth. Scenedesmus quadricauda grew well at a concentration of 0.5 g / L NaNO3. Fatty acid analysis was done by using GC-MS. Scenedesmus quadricauda had several types of fatty acids. The most abundant fatty acid in Scenedesmus quadricaudawas C18: 1.Keywords: Microalgae, Scenedesmus quadricauda, Nile red staining, Isolation, Fatty acid ABSTRAKMikroalga memiliki laju pertumbuhan yang cepat dan mengandung asam lemak sehingga berpotensi sebagai bioenergi. Selain itu, mikroalga juga hidup dengan mengikat karbondioksia untuk proses fotosintesis, sehingga mampu mengurangi pencemaran udara. Pada penelitian ini, sampel mikroalga diambil dari air kolam dari daerah Sawahan, Padang, Sumatera Barat. Pewarnaan nilered dilakukan terhadap sampel untuk melihat potensi kandungan lipid. Mikroalga yang memiliki kandungan lipid diisolasi dengan menggunakan teknik pipet kapiler, kemudian isolat diidentifikasi secara morfologi dengan menggunakan mikroskop. Isolat yang diperoleh diidentifikasi sebagai Scenedesmus quadricauda. Isolat dikultivasi dalam Bold Bassal Medium (BBM). Pertumbuhan mikroalga diukur dengan melihat nilai optical density. Isolat dikultivasi dalam berbagai konsentrasi NaNO3 untuk melihat pengaruh jumlah nitrogen terhadap pertumbuhannya. Scenedesmus quadricauda tumbuh dengan baik pada konsentrasi 0,5 g/L NaNO3. Analisa asam lemak dilakukan dengan menggunakan GC-MS. Pada Scenedesmus quadricauda ditemukan beberapa jenis asam lemak. Asam lemak yang paling banyak terkandung pada mikroalga ini yaitu C18:1.Kata kunci: Mikroalga, Scenedesmus quadricauda, Pewarnaan nile red, Isolasi, Asam lemak

Abstract. Susanty D, Oksari AA. 2020. Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media. Nusantara Bioscience 12: 28-32. Intensive chicken broiler farms create waste in the environment. Chicken Broiler Waste (CBW) was used as culture media for Chlorella sp. InaCC M39 and Chlorella sorokiniana InaCC M38 at various concentrations (2, 4, 6, 8, and 10%), and compared to AF6 media. The growth of Chlorella was observed every 48 hours for 10 days by counting the number of cells using a hemocytometer. The chloroform extract of Chlorella sp. and C. sorokiniana were analyzed for their phytochemical content to determine the presence of alkaloids, flavonoids, saponin, steroids, and tannin. The results showed that 2% of CBW media was the best medium for the growth of Chlorella with nitrogen (N), phosphorus (P), and potassium (K) content was 0.01% N; 0.01% P; 0.06% K respectively. The highest specific growth rate of Chlorella sp. on 2% CBW medium was on the 8th day of culture (0.8133) with cell density of 1.6 x 106 cells/mL, while the highest specific growth rate of Chlorella sorokiniana was on the 6th day (0.8907) with cell density of 2.99 x 107 cells/mL. The chloroform extract of the biomass of Chlorella sorokiniana contained steroid and saponin