Characterization of certain inflammatory variables in the peripheral blood of clinically healthy dogs

Breathnach, Rory; Donahy, C.; Jones, B. R.; Bloomfield, F. J.

doi:10.1016/j.tvjl.2004.09.011

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…The oxidative burst is one of the most important defense mechanisms against infectious diseases and can be stimulated by chemoattractants [3,5]. In the present study, PT was reduced by all priming agents, suggesting that priming agents enhance the responsiveness to sOZ in canine granulocytes.…”

Section: Discussionsupporting

confidence: 55%

Priming Effects of Lipopolysaccharide and Inflammatory Cytokines on Canine Granulocytes

Maeda

Sakonju

Kanda

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. Granulocytes play a pivotal role in natural immunity. Under inflammatory conditions, granulocytes are universally primed by several agents, such as endotoxins and inflammatory cytokines. Primed granulocytes exert potent adhesiveness, chemotaxis, phagocytosis and reactive oxygen species (ROS) production, effectively eliminating invading agents. Reactivity against priming agents is known to vary with species; however, there have been few reports on the effects of priming agents on canine granulocytes. In the present study, we assayed the priming effects of lipopolysaccharide (LPS), recombinant canine tumor necrosis factor- (rcTNF-) and recombinant canine granulocyte macrophage colony-stimulating factor (rcGM-CSF) on canine granulocyte function in vitro. Isolated recombinant canine were primed with various concentrations of LPS, rcTNF- and rcGM-CSF, and CD11b expression was assayed. Furthermore, actin polymerization, phagocytosis and ROS production were then assayed at primer concentrations where enhancement of CD11b expression was observed. LPS did not enhance canine granulocyte function. Phagocytosis and actin polymerization were not enhanced by priming agents; however, rcTNF- and rcGM-CSF enhanced CD11b expression and ROS production in canine granulocytes. These results suggest that priming effects are mainly reflected in CD11b expression and ROS production, with rcGM-CSF and rcTNF- having a priming effect similar to that observed in humans.

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Section: Discussionsupporting

confidence: 55%

Priming Effects of Lipopolysaccharide and Inflammatory Cytokines on Canine Granulocytes

Maeda

Sakonju

Kanda

et al. 2010

The Journal of Veterinary Medical Science

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“…Complement receptor 3 (CR3) is one such receptor, comprising a heterodimer of CD11b and CD18 that recognizes opsonized particles, indicating that CD11b expression is a good marker of cell activation [28]. Furthermore, the oxidative burst is one of the most important defense mechanisms against infectious diseases and can be stimulated by chemoattractants [6,8]. The priming effects of cytokines are primarily reflected in CD11b expression and the oxidative burst.…”

mentioning

confidence: 99%

Effects of Lidocaine Hydrochloride on Canine Granulocytes, Granulocyte CD11b Expression and Reactive Oxygen Species Production

Maeda

Sakonju

Kumakura

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. Lidocaine hydrochloride (Lido) is widely used for analgesia in veterinary medicine; however, in humans, it has been suggested that Lido attenuates granulocyte functions, such as adhesion and reactive oxygen species (ROS) production. Thus, Lido may affect canine granulocyte function; however, there have been no reports on the effects of Lido on canine granulocyte function. Thus, we studied the effects of Lido on canine granulocyte CD11b expression and ROS production. We further studied the effects of Lido on the priming of canine granulocyte CD11b expression and ROS production by recombinant canine granulocyte macrophage colony stimulating factor (rcGM-CSF). Isolated granulocytes were incubated with 3, 30 or 300 g/ml Lido, or with Lido followed by priming with 5 ng/ml rcGM-CSF. CD11b was detected by the immune fluorescent antibody method, and the mean fluorescence intensity (MFI) was assayed by flow cytometry. ROS production was assessed by the peak time (PT) of ROS production and area under the luminol reaction curve (AUC), which represents total ROS production quantity against opsonized zymosan stimuli. Only 300 g/ml Lido (tissue level observed by regional block) significantly attenuated both the MFI of CD11b and its enhancement by rcGM-CSF. Moreover, at this concentration, the AUC and its enhancement by rcGM-CSF were significantly attenuated by Lido; in contrast, Lido did not affect PT. In conclusion, Lido suppressed granulocyte adhesion to the endothelium and antiseptic capability by suppressing CD11b expression and/or ROS production. Particular care should thus be exercised when performing regional anesthesia block using Lido.

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Increased leukotriene B4 production, complement C3 conversion and acid hydrolase enzyme concentrations in different leucocyte sub-populations of dogs with atopic dermatitis

Breathnach

Donahy

Jones

et al. 2006

The Veterinary Journal

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Characterization of certain inflammatory variables in the peripheral blood of clinically healthy dogs

Cited by 3 publications

References 27 publications

Priming Effects of Lipopolysaccharide and Inflammatory Cytokines on Canine Granulocytes

Priming Effects of Lipopolysaccharide and Inflammatory Cytokines on Canine Granulocytes

Effects of Lidocaine Hydrochloride on Canine Granulocytes, Granulocyte CD11b Expression and Reactive Oxygen Species Production

Increased leukotriene B4 production, complement C3 conversion and acid hydrolase enzyme concentrations in different leucocyte sub-populations of dogs with atopic dermatitis

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