Characterization of cell walls in bean (Phaseolus vulgaris L.) callus cultures tolerant to dichlobenil

Encina, Antonio; Moral, Rosa; Acebes, José Luis; Álvarez, Jesús Miguel Muñoz

doi:10.1016/s0168-9452(00)00397-6

Cited by 33 publications

(56 citation statements)

References 27 publications

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“…The decrease in pectin DM has been reported in several mutants with disrupted cellulose synthesis or modified cellulose ultrastructure (Shedletzky et al, 1990;Encina et al, 2001;Manfield et al, 2004;Sullivan et al, 2011). It has been suggested that calcium-mediated cross linking of HG can compensate, to some extent, for the reduced load-bearing capacity of the disrupted cellulosic network.…”

Section: Modulation Of Mucilage Structure By Glucomannanmentioning

confidence: 94%

CELLULOSE SYNTHASE-LIKE A2, a Glucomannan Synthase, Is Involved in Maintaining Adherent Mucilage Structure in Arabidopsis Seed

Shi

et al. 2014

Plant Physiol.

135

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Mannans are hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall. However, it is not yet known how mannans function in Arabidopsis (Arabidopsis thaliana) seed mucilage. In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats. Disruption of CSLA2 resulted in thinner adherent mucilage halos, although the total amount of the adherent mucilage did not change compared with the wild type. This suggested that the adherent mucilage in the mutant was more compact compared with that of the wild type. In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type. No appreciable changes in the composition, structure, or macromolecular properties were observed for nonmannan polysaccharides in mutant mucilage. Biochemical analysis revealed that cellulose crystallinity was substantially reduced in csla2-1 mucilage; this was supported by the removal of most mucilage cellulose through treatment of csla2-1 seeds with endo-b-glucanase. Mutation in CSLA2 also resulted in altered spatial distribution of cellulose and an absence of birefringent cellulose microfibrils within the adherent mucilage. As with the observed changes in crystalline cellulose, the spatial distribution of pectin was also modified in csla2-1 mucilage. Taken together, our results demonstrate that glucomannans synthesized by CSLA2 are involved in modulating the structure of adherent mucilage, potentially through altering cellulose organization and crystallization.Mannan polysaccharides are a complex set of hemicellulosic cell wall polymers that are considered to have both structural and storage functions. Based on the particular chemical composition of the backbone and the side chains, mannan polysaccharides are classified into four types: pure mannan, glucomannan, galactomannan, and galactoglucomannan (Moreira and Filho, 2008;Wang et al., 2012;Pauly et al., 2013). Each of these polysaccharides is composed of a b-1,4-linked backbone containing Man or a combination of Glc and Man residues. In addition, the mannan backbone can be substituted with side chains of a-1,6-linked Gal residues. Mannan polysaccharides have been proposed to cross link with cellulose and other hemicelluloses via hydrogen bonds (Fry, 1986;Iiyama et al., 1994;Obel et al., 2007;Scheller and Ulvskov, 2010). Furthermore, it has been reported that heteromannans with different levels of substitution can interact with cellulose in diverse ways (Whitney et al., 1998). Together, these observations indicate the complexity of mannan polysaccharides in the context of cell wall architecture.CELLULOSE SYNTHASE-LIKE A (CSLA) enzymes have been shown to have mannan synthase activity in vitro. These enzymes polymerize the b-1,4-linked backbone of mannans or glucomannans, depending on the substrates (GDP-Man and/or GDP-Glc) provided (Richmond and Some...

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Section: Modulation Of Mucilage Structure By Glucomannanmentioning

confidence: 94%

CELLULOSE SYNTHASE-LIKE A2, a Glucomannan Synthase, Is Involved in Maintaining Adherent Mucilage Structure in Arabidopsis Seed

Shi

et al. 2014

Plant Physiol.

135

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“…29 Later, the process of habituation of bean callus cells was analyzed, comparing the spectra from non-habituated callus cell walls with those from habituated to different DCB concentrations up to 12 μM callus cell walls. 30 The results obtained for FTIR spectra from habituated cells showed that as the concentration of herbicide increased, so did the peaks corresponding to uronic acids, free carboxylic acid groups and ester links.…”

Section: Using Ftir To Monitor the Effects Of Cbismentioning

confidence: 97%

The use of FTIR spectroscopy to monitor modifications in plant cell wall architecture caused by cellulose biosynthesis inhibitors

Alonso‐Simón

García‐Angulo²,

Mélida³

et al. 2011

Plant Signaling & Behavior

103

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“…The habituation of bean calluses to dichlobenil was associated with a high scavenging capacity of ROS, mainly by CIII-POX activity (García-Angulo et al, 2009) and also with the capacity of the cells to divide and expand with a modified cell wall in which the xyloglucan-cellulose network had been partially replaced by pectins (Encina et al, 2001(Encina et al, , 2002. However, quinclorac-habituated bean cells have a non-modified cell wall (Alonso-Simón et al, 2008) and, as was observed in this study, this habituation seemed to be related to a high antioxidant capacity.…”

Section: Discussionmentioning

confidence: 99%

“…Bean (Phaseolus vulgaris L.) cell lines were obtained and subcultured as described by Encina et al (2001) on Murashige and Skoog (1962) solid basal medium supplemented with sucrose (30 g L −1 ), 10 μM 2,4-D (2,4-dichlorophenoxyacetic acid) and agar (8 g L −1 ).…”

Section: Plant Materials and Quinclorac Habituationmentioning

confidence: 99%

“…In addition, modifications in cell wall composition due to the habituation process were analysed (Alonso-Simón et al, 2008). The results obtained showed that the mechanism of bean cells habituation to quinclorac differed from that reported for well-known CBIs such as dichlobenil (Encina et al, 2001(Encina et al, , 2002 or isoxaben (Díaz-Cacho et al, 1999). In the dichlobenil and isoxaben-habituation processes, bean cells developed the capacity to divide and expand, with a modified cell wall in which the xyloglucan-cellulose network had been partially replaced by pectins.…”

Section: Introductionmentioning

confidence: 99%

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Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

Largo-Gosens

Castro

Alonso‐Simón

et al. 2016

Plant Physiology and Biochemistry

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The habituation of bean cells to quinclorac did not rely on cell wall modifications, contrary to what it was previously observed for the well-known cellulose biosynthesis inhibitors dichlobenil or isoxaben. The aim of the present study was to investigate whether or not the bean cells habituation to quinclorac is related to an enhancement of antioxidant activities involved in the scavenging capacity of reactive oxygen species. Treating non-habituated bean calluses with 10 μM quinclorac reduced the relative growth rate and induced a two-fold increase in lipid peroxidation. However, the exposition of quinclorac-habituated cells to a concentration of quinclorac up to 30 μM neither affected their growth rate nor increased their lipid peroxidation levels. Quinclorac-habituated calluses had significantly higher constitutive levels of three antioxidant activities (class-III peroxidase, glutathione reductase, and superoxide dismutase) than those observed in non-habituated calluses, and the treatment of habituated calluses with 30 μM quinclorac significantly increased the level of class III-peroxidase and superoxide dismutase. The results reported here indicate that the process of habituation to quinclorac in bean callus-cultured cells is related, at least partially, to the development of a stable antioxidant capacity that enables them to cope with the oxidative stress caused by quinclorac. Class-III peroxidase and superoxide dismutase activities could play a major role in the quinclorac-habituation. Changes in the antioxidant status of bean cells were stable, since the increase in the antioxidant activities were maintained in quinclorac-dehabituated cells.

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Characterization of cell walls in bean (Phaseolus vulgaris L.) callus cultures tolerant to dichlobenil

Cited by 33 publications

References 27 publications

CELLULOSE SYNTHASE-LIKE A2, a Glucomannan Synthase, Is Involved in Maintaining Adherent Mucilage Structure in Arabidopsis Seed

CELLULOSE SYNTHASE-LIKE A2, a Glucomannan Synthase, Is Involved in Maintaining Adherent Mucilage Structure in Arabidopsis Seed

The use of FTIR spectroscopy to monitor modifications in plant cell wall architecture caused by cellulose biosynthesis inhibitors

Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

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