The use of FTIR spectroscopy to monitor modifications in plant cell wall architecture caused by cellulose biosynthesis inhibitors

Alonso‐Simón, Ana; García‐Angulo, Penélope; Mélida, Hugo; Encina, Antonio; Álvarez, Jesús Miguel Muñoz; Acebes, José Luis

doi:10.4161/psb.6.8.15793

Cited by 104 publications

(63 citation statements)

References 45 publications

(43 reference statements)

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“…Averaged difference spectra were obtained by digital subtraction of SNH spectra from each of the DCB‐treated/habituated cell lines (Figure ). Compared with SNH spectra, those from both short‐term treated and habituated cell walls showed negative peaks in the region ranging from 900 to 1,200 cm −1 where most of the cell wall polysaccharides, including cellulose, absorb (Alonso‐Simón et al ; Largo‐Gosens et al ). In addition, positive peaks were detected associated with wavenumbers indicative of aromatic rings (1,515, 1,600 and 1,630 cm −1 ), phenolic rings (1,500 cm −1 ) and phenolic esters (1,720 cm −1 ) (Kačuráková et al ), indicating that both DCB‐treated and DCB‐habituated cells were enriched in phenolics.…”

Section: Resultsmentioning

confidence: 99%

Ectopic lignification in primary cellulose‐deficient cell walls of maize cell suspension cultures

Mélida

Largo-Gosens

Novo-Uzal

et al. 2015

JIPB

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Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor. Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy. Cell wall compositional analysis indicated that the cellulosedeficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol. In accordance with this, cellulose-deficient cell walls showed a fivefold increase in Klason-type lignin. Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to shortterm DCB-treatments. Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin. A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions. In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.Keywords: Cellulose; DCB; dichlobenil; ectopic lignin; maize Citation: M elida H, Largo-Gosens A, Novo-Uzal E, Santiago R, Pomar F, Garc ıa P, Garc ıa-Angulo P, Acebes JL, Alvarez J, Encina A (2015) Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures.

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Section: Resultsmentioning

confidence: 99%

Ectopic lignification in primary cellulose‐deficient cell walls of maize cell suspension cultures

Mélida

Largo-Gosens

Novo-Uzal

et al. 2015

JIPB

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“…Although ATR-FTIR spectroscopy is not similarly precise in quantitative analysis of a specific analyte like time-consuming chromatography, it does allow for comprehensive survey of all essential compounds present in the material under investigation on the basis of a single short-time measurement. ATR-FTIR spectroscopy is generally used in combination with various mathematical and statistical tools that enhance its research potential, e.g., to discriminate between samples according to taxonomic categories [33,34] or to determine susceptibility to stresses [35][36][37]. Additionally, mediation analysis is often used as a method of choice to study the relationship between distinct compounds [38] as it does not involve expensive molecular experiments and may support valuable data on biological mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

Bednarek

Żebrowski

Orłowska

2020

IJMS

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Tissue culture is an essential tool for the regeneration of uniform plant material. However, tissue culture conditions can be a source of abiotic stress for plants, leading to changes in the DNA sequence and methylation patterns. Despite the growing evidence on biochemical processes affected by abiotic stresses, how these altered biochemical processes affect DNA sequence and methylation patterns remains largely unknown. In this study, the methylation-sensitive Amplified Fragment Length Polymorphism (metAFLP) approach was used to investigate de novo methylation, demethylation, and sequence variation in barley regenerants derived by anther culture. Additionally, we used Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy to identify the spectral features of regenerants, which were then analyzed by mediation analysis. The infrared spectrum ranges (710–690 and 1010–940 cm−1) identified as significant in the mediation analysis were most likely related to β-glucans, cellulose, and S-adenosyl-L-methionine (SAM). Additionally, the identified compounds participated as predictors in moderated mediation analysis, explaining the role of demethylation of CHG sites (CHG_DMV) in in vitro tissue culture-induced sequence variation, depending on the duration of tissue culture. The data demonstrate that ATR-FTIR spectroscopy is a useful tool for studying the biochemical compounds that may affect DNA methylation patterns and sequence variation, if combined with quantitative characteristics determined using metAFLP molecular markers and mediation analysis. The role of β-glucans, cellulose, and SAM in DNA methylation, and in cell wall, mitochondria, and signaling, are discussed to highlight the putative cellular mechanisms involved in sequence variation.

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“…3A). We subjected mutants and wild-type purified cell walls to Fourier-Transform InfraRed (FTIR) spectroscopy that can assign wall polymers and functional groups to different wavenumbers of the FTIR-spectra (57). Differential FTIR-spectra obtained after digital subtraction of the wild-type values from the mutants, showed clearly that xcp2-1, namt2-1, acs8-2, at1g70770-1, at1g23170-1 and ago4-1t were cell P l a n t c e l l w a l l d e t e r m i n e s r e s i s t a n c e a n d f i t n e s s 8 wall mutants with biochemical alterations that differ from those observed in the previously characterized det3-1, irx1-6, irx10-1 and arr6-3 wall mutants ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Arabidopsiscell wall composition determines disease resistance specificity and fitness

Molina

Miedes

Bacete

et al. 2020

Preprint

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Plant cell walls are complex structures subject to dynamic remodeling in response to developmental and environmental cues, and play essential functions in disease resistance responses. We tested the specific contribution of plant cell walls to immunity by determining the susceptibility of a set of Arabidopsis cell wall mutants (cwm) to pathogens with different parasitic styles: a vascular bacterium, a necrotrophic fungus and a biotrophic oomycete. Remarkably, most cwm mutants tested (31/38; 81.6%) showed alterations in their resistance responses to at least one of these pathogens, in comparison to wild-type plants, illustrating the relevance of wall composition in determining disease resistance phenotypes. We found that the enhanced resistance of cwm plants to the necrotrophic and vascular pathogens negatively impacted on cwm fitness traits, like biomass and seed yield. Enhanced resistance of cwm plants is not only mediated by canonical immune pathways, like those modulated by phytohormones or Microbe-Associated Molecular Patterns, which are not de-regulated in all cwm tested. Pectin-enriched wall fractions isolated from cwm plants triggered immune responses in other plants, suggesting that wall-mediated defensive pathways might contribute to cwm resistance. Cell walls of cwm plants show a high diversity of composition alterations as revealed by glycome profiling that detect specific wall carbohydrate moieties. Mathematical analysis of glycome profiling data identified correlations between the amounts of specific wall carbohydrate moieties and disease resistance phenotypes of cwm plants. These data support the relevant and specific function of plant wall composition in plant immune response modulation and in balancing disease resistance/development trade-offs.

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The use of FTIR spectroscopy to monitor modifications in plant cell wall architecture caused by cellulose biosynthesis inhibitors

Cited by 104 publications

References 45 publications

Ectopic lignification in primary cellulose‐deficient cell walls of maize cell suspension cultures

Ectopic lignification in primary cellulose‐deficient cell walls of maize cell suspension cultures

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

Arabidopsiscell wall composition determines disease resistance specificity and fitness

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