Characterization of carbosilane dendrimers as effective carriers of siRNA to HIV-infected lymphocytes

Potential inhibition of HIV-1 encapsidation by oligoribonucleotide–dendrimer nanoparticle complexes

“…MT4 lymphocytes exposed to the dendriplex retained .80% viability at a dendrimer concentration of 15 µM (equivalent to 138 µg/mL). By comparison, peripheral blood mononuclear cells and SupT1 cells exposed to a second-generation carbosilane dendrimer in a study by Weber et al 56 reached the toxicity limit of 80% at 24 µg/mL, while in another study, two second-generation dendrimers reached the toxicity limit for macrophages at 5 and 15 µM, respectively. 33 …”

“…56 The cells were harvested, washed first with PBS, then with acidglycine buffer for 30 seconds and finally again with PBS. The acid-glycine buffer (for the removal of dendriplexes bound to the surface of the cell membranes) consisted of 0.2 M glycine in PBS (adjusted to pH 3.0 by addition of hydrochloric acid).…”

Dendrimer Structure Diversity and Tailorability as a Way to Fight Infectious Diseases

“…Carbosilane dendrimers were also investigated in terms of other potential antiviral applications [62,63]. Knowing that dendrimers are excellent non-viral transfection agents, two polycationic G1 carbosilane dendrimers with different cores were assessed for gene therapy in order to inhibit the development of ongoing HIV infection.…”

Antiviral mechanism of polyanionic carbosilane dendrimers against HIV-1

“…[8][9][10] In the last few years, nanotechnology offered a huge battery of novel nanoparticles such as dendrimers and macromolecules characterized by hyperbranched, well-defined, monodisperse, three-dimensional structures that are being developed as drug delivery vehicles or as therapeutic agents. [11][12][13][14][15][16][17] The controlled synthesis of dendrimers allows the assembly of highly defined, single molecule structures that radiate out in branches from a central initiator core. The type of core and branching units can be engineered to generate a broad variety of dendrimers with different sizes, shapes, and surface chemical functionalized groups that can provide distinct biological and pharmacological properties.…”