Characterization of ATPases of apical membrane fractions from Locusta migratoria Malpighian tubules

Al-Fifi, Zarraq; Marshall, Steve; Hyde, David; Anstee, J.H.; Bowler, K.

doi:10.1016/s0965-1748(98)00025-3

Cited by 19 publications

(8 citation statements)

References 51 publications

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“…MT dissected from control and freeze-treated groups were either immediately homogenized in an ice-cold homogenization buffer containing 250·mmol·l -1 sucrose and 5·mmol·l -1 imidazole, pH·7.4 (Al-Fifi et al, 1998) or stored in 100·µl of the same buffer containing 10% DMSO (dimethyl sulfoxide) at -80°C overnight. Membrane preparations were conducted with a modified procedure based on the methods described by Al-Fifi et al (1998), Crockett and Hazel (1995), Fogg et al (1991) and Sørensen (1981Sørensen ( , 1993.…”

Section: Membrane Preparationmentioning

confidence: 99%

“…Membrane preparations were conducted with a modified procedure based on the methods described by Al-Fifi et al (1998), Crockett and Hazel (1995), Fogg et al (1991) and Sørensen (1981Sørensen ( , 1993. Briefly, tissues were first homogenized in a glass homogenizer with the homogenization buffer (1·mg tissue in 10·µl buffer), and then processed with an Ultrasonic Processor (Cole-Parmer Instrument Co., Vernon Hill, IL, USA) for 10·s, three times, with an Amplitude setting at 40.…”

Section: Membrane Preparationmentioning

confidence: 99%

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Changes in gut and Malpighian tubule transport during seasonal acclimatization and freezing in the gall flyEurosta solidaginis

Lee

2005

Journal of Experimental Biology

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SUMMARY Since few studies have examined cold tolerance at the organ level in insects, our primary objective was to characterize the functional responses of the gut and Malpighian tubules (MT) to seasonal acclimatization, chilling and freezing in larvae of the goldenrod gall fly Eurosta solidaginisFitch (Diptera, Tephritidae). From September to December, hemolymph osmolality(455-926 mOsmol kg l-1) and freezing tolerance increased markedly in field-collected larvae. Chlorophenol Red was readily transported into the lumen of the foregut, the posterior portion of the midgut, the ureter, the proximal region of the anterior pair of MT, and entire posterior pair of MT. Ouabain and KCN inhibited transport of Chlorophenol Red in the gut and MT. Transport was readily detected at 0°C and the rate of transport was directly related to temperature. The rate of fluid transport by the MT decreased steadily from a monthly high in September (10.7±0.8 nl min-1 for the anterior pair; 12.7±1.0 nl min-1for the posterior pair) until secretion was no longer detectable in December;this decrease parallels entry into diapause for this species. Even in larvae that died following freezing for 40 days at -20°C, individual organ function was retained to a limited extent. Through the autumn, cholesterol concentrations in the hemolymph increased nearly fourfold. In contrast, the ratio of cholesterol to protein content (nmol mg l-1) in the MT membrane remained relatively constant (22∼24 nmol mg l-1protein) during this period. Freezing of larvae for 20 days at -20°C caused a significant decrease in cholesterol levels in the hemolymph and the MT membranes compared to unfrozen controls. These results suggest that cholesterol plays a role in seasonal cold hardening and freeze tolerance in insects.

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Section: Membrane Preparationmentioning

confidence: 99%

Section: Membrane Preparationmentioning

confidence: 99%

Changes in gut and Malpighian tubule transport during seasonal acclimatization and freezing in the gall flyEurosta solidaginis

Lee

2005

Journal of Experimental Biology

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“…Osmolality was checked using an osmometer (Knauer, Berlin, Germany). Cells were cultured for 67 days and treated with LiCl; SB216763, a potent inhibitor of both GSK3 isoforms [17] (Sigma, Deisenhofen, Germany); TWS119, a potent inhibitor of the GSK3β isoform [15] (Millipore, Darmstadt, Germany); bafilomycin A1 and chloroquine, both inhibitors of the vacuolar ATPase [18,19] and thereby lysosomal activity inhibitors; MG132, as a proteasome inhibitor [20]; and dbcAMP (all from Sigma, Deisenhofen, Germany) in different concentrations and durations, alone or together as indicated. All experiments were conducted with at least 3 independent cell cultures from different rats.…”

Section: Cell Culturementioning

confidence: 99%

Lithium Chloride and GSK3 Inhibition Reduce Aquaporin-2 Expression in Primary Cultured Inner Medullary Collecting Duct Cells Due to Independent Mechanisms

Kaiser

Edemir

2020

Cells

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Lithium chloride (LiCl) is a widely used drug for the treatment of bipolar disorders, but as a side effect, 40% of the patients develop diabetes insipidus. LiCl affects the activity of the glycogen synthase kinase 3 (GSK3), and mice deficient for GSK3β showed a reduction in the urine concentration capability. The cellular and molecular mechanisms are not fully understood. We used primary cultured inner medullary collecting duct cells to analyze the underlying mechanisms. LiCl and the inhibitor of GSK3 (SB216763) induced a decrease in the aquaporin-2 (Aqp2) protein level. LiCl induced downregulation of Aqp2 mRNA expression while SB216763 had no effect and TWS119 led to increase in expression. The inhibition of the lysosomal activity with bafilomycin or chloroquine prevented both LiCl-and SB216763-mediated downregulation of Aqp2 protein expression. Bafilomycin and chloroquine induced the accumulation of Aqp2 in lysosomal structures, which was prevented in cells treated with dibutyryl cyclic adenosine monophosphate (dbcAMP), which led to phosphorylation and membrane localization of Aqp2. Downregulation of Aqp2 was also evident when LiCl was applied together with dbcAMP, and dbcAMP prevented the SB216763-induced downregulation. We showed that LiCl and SB216763 induce downregulation of Aqp2 via different mechanisms. While LiCl also affected the mRNA level, SB216763 induced lysosmal degradation. Specific GSK3β inhibition had an opposite effect, indicating a more complex regulatory mechanism.

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“…Fat body (FB) and midgut (MG) tissues and Malpighian tubules (MT) were dissected in an ice-cold homogenization buffer containing 250 mmol l −1 sucrose and 5 mmol l −1 imidazole, pH 7.4 (Al-Fifi et al, 1998). Larvae were pinned dorsal-side up in a silicone elastomer (Dow Corning Sylgard 184)-filled Petri dish and opened with a midline incision.…”

Section: Tissue Dissection and Membrane Preparationmentioning

confidence: 99%

“…Membrane preparations were conducted with a procedure based on the methods described by Al-Fifi et al (1998), Crockett and Hazel (1995a), Fogg et al (1991) and Sørensen (1981Sørensen ( , 1993. Briefly, tissues were first homogenized in a glass homogenizer with the homogenization buffer (1 mg tissue in 10 µl buffer), and then processed with an Ultrasonic Processor (Cole-Parmer Instrument Co., Vernon Hill, IL, USA) three times for 10 s with an amplitude setting at 40.…”

Section: Tissue Dissection and Membrane Preparationmentioning

confidence: 99%

Cold-hardening during long-term acclimation in a freeze-tolerant woolly bear caterpillar, Pyrrharctia isabella

Lee

2015

Journal of Experimental Biology

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The banded woolly bear caterpillar, Pyrrharctia isabella (Lepidoptera: Erebidae), overwinters in leaf litter and survives freezing under natural conditions. Following 18 weeks of cold acclimation at 5°C, all caterpillars could survive 1 week of continuous freezing at −20°C or seven cycles of freezing-thawing at −20°C, but none survived freezing at −80°C. Field-collected caterpillars had a temperature of crystallization of −7.7±0.5°C that decreased significantly to −9.5±0.6°C after 12 weeks of acclimation at 5°C. Hemolymph levels of free proline, total amino acids and proteins reached a peak during the first 4 weeks of acclimation; concomitantly, hemolymph osmolality increased markedly during this interval (from 364 to 1282 mosmol kg −1). In contrast, hemolymph pH decreased during the first 4 weeks of acclimation before this trend reversed and pH values gradually returned to initial values. However, pH reached its peak value following 1 week at −20°C, but decreased after longer periods of freezing. During cold acclimation, cholesterol levels decreased in the hemolymph and the membrane fraction of fat body but not in other tissues. Lethal freezing at −80°C reduced cell survival in foregut tissue and caused leakage of free proline, total amino acids and proteins from tissues into the hemolymph. The addition of glycerol to the bathing medium reduced freezing injury in fat body cells, as evidenced by reduced leakage of amino acids and proteins.

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Characterization of ATPases of apical membrane fractions from Locusta migratoria Malpighian tubules

Cited by 19 publications

References 51 publications

Changes in gut and Malpighian tubule transport during seasonal acclimatization and freezing in the gall flyEurosta solidaginis

Changes in gut and Malpighian tubule transport during seasonal acclimatization and freezing in the gall flyEurosta solidaginis

Lithium Chloride and GSK3 Inhibition Reduce Aquaporin-2 Expression in Primary Cultured Inner Medullary Collecting Duct Cells Due to Independent Mechanisms

Cold-hardening during long-term acclimation in a freeze-tolerant woolly bear caterpillar, Pyrrharctia isabella

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