Characterization of Antimicrobial, Antioxidant, Anticancer Property and Chemical Composition of <i>Michelia champaca</i> Seed and Flower Extracts

Abstract

This study was carried out to characterize antimicrobial, antioxidant and anticancer activities of Michelia champaca seed and flower extracts. The main objective of the present study was to reveal the medicinal values of M. champaca seed and flower for human uses. Antimicrobial property of M. champaca seed and flower extracts were revealed by using two fold microdilution method whereas antioxidant activity of the extract was determined with DPPH radical scavenging method. The anticancer property of the plant extract was revealed through Colorimetric MTT (tetrazolium) assay. The minimum inhibitory concentration values of M. champaca seed and flower extracts ranged from 15.6 to 125mg/l and 7.8 to 62.5mg/l, respectively in which both of the plant extracts were found can inhibit the growth of all the tested bacterial isolates namely A. hydrophila, E. tarda, E. coli, Flavobacterium sp., Klebsiella sp., P. aeruginosa, Salmonella sp., V. alginolyticus, V. cholerae and V. parahaemolyticus. M. champaca flower extract was able to control the growth of E. tarda, E. coli, Flavobacterium sp., P. aeruginosa and V. cholerae at the concentration of 7.8mg/l whereas A. hydrophila, Klebsiella sp. and V. alginolyticus were failed to grow at the concentration 15.6mg/l. The M. champaca flower extract was also able to control the growth of Salmonella sp. and V. parahaemolyticus at the concentration of 62.5mg/l. At the maximum concentration of M. champaca seed and flower extracts were found can inhibit only 40% of DPPH whereas the IC50 value of M. champaca seed and flower extract against MCF-7 cells was 1.98 ±0.31µg/ml and 1.86 ± 0.21µg/ml, respectively. A total of 9 chemical compounds were successfully identified in M. champaca's flower extract whereas 37 chemical compounds were found in the leaf extract. The findings of the present study indicated that medicinal values of M. champaca seed & flower extracts in terms of antimicrobial and anticancer are promising.

The wound healing property of ethanolic extract of &lt;i&gt;Michelia champaca&lt;/i&gt; flowers in diabetic rats

“…Although the earlier studies have showed the presence of several phytochemicals in the flower extracts, 29,30 it is not yet clear, which constituents of the extract of MC are responsible for its beneficial effect in diabetic wounds.…”

“…Many plant extracts and herbs, which have antioxidant effect have proved to be beneficial in treating complications resulting from diabetes. 36 There have been several studies that have reported antioxidant properties of MC, 30 which suggest the beneficial effect of this plant in wound healing in diabetics.…”

“…38 Furthermore, wounds in diabetics have a higher propensity to become infected, which may impede the progress or completion of healing. 39 There have been several studies which have demonstrated the antimicrobial property of flower extracts of MC, 30,40 which may play a role in enhanced wound healing in the treated animals.…”

Restoration of Memory and Acetylcholinesterase Activity by Michelia Champaca in Chronically Noise Stressed Wistar Albino Rats

“…More recently, there has been a greater search for natural therapies. M. champaca is a folk-medicinal plant, well known for its antioxidant and antidiabetic property, [5] it also has other numerous properties such as analgesic, anti-inflammatory, and [6] anticancer activities [5]. These unique effects may be due to the presence of phytochemicals, such as phenols, saponins, tannins, amino acids, flavonoids, terpenoids, and catecholamines, [7] involved in biological manipulations and activities.…”

Role of Michelia Champaca in Memory Enhancement and Acute Noise Stressed Male Wistar Albino Rats

“…The drugs of plant origin are gaining increasing popularity and are being investigated for remedies of several disorders. M. champaca is a folk-medicinal plant, well known for its antioxidant and antidiabetic property, [12] it also has other numerous properties such as analgesic, anti-inflammatory, and [13] anticancer activities [14]. These unique effects are due to the presence of compounds like phenols, saponins, tannins, amino acids, flavonoids, terpenoids, and catecholamines, [15] involved in biological manipulations and activities.…”