Characterization of antibiotic resistant and pathogenic Escherichia coli in irrigation water and vegetables in household farms

Araújo, Susana; Silva, Isabel; Tacão, Marta; Patinha, Carla; Alves, Artur; Henriques, Isabel

doi:10.1016/j.ijfoodmicro.2017.06.020

Cited by 101 publications

(71 citation statements)

References 63 publications

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“…Among the total amount of presumptive E. coli isolates (238), the quantity of positively con rmed E. coli isolates (192/83%) was unsurprisingly very high and this was similar to other studies by Blaak et al [42] and Maamar et al [43] with both reporting high incidence of E. coli isolates from farm environments. In another study by Araújo et al [44], E. coli was isolated from irrigation water as well as vegetable samples and this is in line with our ndings. This study provides an evidence of the occurrence of pathogenic microorganisms in the environment linking to other niches and our results show the occurrence of CREC in farm samples (Fig.…”

Section: Discussionsupporting

confidence: 93%

Genetic Characterization Among Carbapenem-resistant Escherichia coli Strains Using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) Fingerprinting in South Africa

Ebomah¹,

Okoh²

2020

Preprint

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Background Carbapenems belong to beta-lactam class of antibiotics usually considered as the last line of defense because they can be effective against severe infections caused by prevalent multidrug-resistant (MDR) pathogens. However, carbapenems can be deactivated by bacteria that produce carbapenemase (beta-lactamase). This study was conducted to screen for carbapenem-resistance genes (CRGs) harbored by pathogenic strains of Escherichia coli recovered from different environmental samples. We also assessed the genetic relatedness among selected E. coli pathotypes using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR).Method: Molecular identification and characterization of the presumptive isolates were performed using PCR and isolates that exhibited antimicrobial resistance (AMR) phenotypically were further screened for some relevant CRGs (blaNDM−1, blaKPC and blaOXA−48−like). Furthermore, ERIC-PCR was used to determine the similarity and diversity of 31 E. coli strains which were randomly selected from the different sources analyzed in this study.Result Our findings revealed a total of 238 presumptive E. coli isolates, out of which 192 were confirmed positive for uidA gene. Further screening revealed 77 (40%) isolates belong to six key E. coli pathotypes and 70 of them exhibited phenotypic AMR. Additionally, twenty-nine (41%) of the 70 MDR pathogenic E. coli strains harbored CRGs; with 24 strains harboring blaNDM−1, 8 harboring blaKPC and 2 harboring blaOXA−48−like genes.Conclusion Findings also suggest that the selected E. coli pathotypes belonged to different genomic clusters, while the cluster analysis showed a possible genetic diversity among aquatic and farm isolates. Proper treatment of final effluents before discharge as well as the development of more effective strategies to control and manage the use of antimicrobial agents were strongly recommended.

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Section: Discussionsupporting

confidence: 93%

Genetic Characterization Among Carbapenem-resistant Escherichia coli Strains Using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) Fingerprinting in South Africa

Ebomah¹,

Okoh²

2020

Preprint

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“…We have observed much higher levels of antibiotic resistance (61·6%) in E. coli as compared to previously reported studies in fresh vegetable‐isolated E. coli strains (Araújo et al . ). Holvoet et al .…”

Section: Discussionmentioning

confidence: 97%

“…Holvoet et al (2013) have also observed moderate level of antibiotic resistance (45%) in E. coli strains isolated from lettuce. Interestingly, we have observed that several isolates of E. coli were resistant against critically important antibiotics including CIP and OFX, which are routinely employed in clinical settings and to which resistance in vegetables is rare (Ibekwe and Grieve 2003;Ara ujo et al 2017). Furthermore, we detected moderate levels of drug resistance (38Á5%) in Salmonella, which is consistent with several previous findings (Duffy et al 2005;Gomba et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and virulence traits ofEscherichia coliandSalmonellastrains isolated from vegetables and fruits from India

et al. 2018

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“…A Portuguese study found a fairly even distribution between B1, A and D in ready-to-eat salads (Campos et al, 2013). In another Portuguese study, phylogroups A and B1 were the most prevalent both in irrigation water and vegetables (Araujo et al, 2017). This suggests that phylogroups of E. coli from produce exhibit distributions that differ geographically and are influenced by the type of produce sampled.…”

Section: Phylogroup B1 and A Selection Of Sequence Types Dominate Promentioning

confidence: 99%

Whole Genome Sequencing of Escherichia coli From Store-Bought Produce

et al. 2020

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The role of agriculture in the transfer of drug resistant pathogens to humans is widely debated and poorly understood. Escherichia coli is a valuable indicator organism for contamination and carriage of antimicrobial resistance (AMR) in foods. Whilst whole genome sequences for E. coli from animals and associated meats are common, sequences from produce are scarce. Produce may acquire drug resistant E. coli from animal manure fertilizers, contaminated irrigation water and wildlife, particularly birds. Whole genome sequencing was used to characterize 120 tetracycline (TET) resistant E. coli from store-bought, ready-to-eat cilantro, arugula and mixed salad from two German cities. E. coli were recovered on the day of purchase and after 7 days of refrigeration. Cilantro was far more frequently contaminated with TET-resistant E. coli providing 102 (85%) sequenced strains. Phylogroup B1 dominated the collection (n = 84, 70%) with multi-locus sequence types B1-ST6186 (n = 37, 31%), C-ST165 (n = 17, 14%), B1-ST58 (n = 14, 12%), B1-ST641 (n = 8, 7%), and C-ST88 (n = 5, 4%) frequently identified. Notably, seven strains of diverse sequence type (ST) carried genetic indicators of ColV virulence plasmid carriage. A number of previously identified and novel integrons associated with insertion elements including IS26 were also identified. Storage may affect the lineages of E. coli isolated, however further studies are needed. Our study indicates produce predominantly carry E. coli with a commensal phylogroup and a variety of AMR and virulence-associated traits. Genomic surveillance of bacteria that contaminate produce should be a matter of public health importance in order to develop a holistic understanding of the environmental dimensions of AMR.

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Characterization of antibiotic resistant and pathogenic Escherichia coli in irrigation water and vegetables in household farms

Cited by 101 publications

References 63 publications

Genetic Characterization Among Carbapenem-resistant Escherichia coli Strains Using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) Fingerprinting in South Africa

Genetic Characterization Among Carbapenem-resistant Escherichia coli Strains Using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) Fingerprinting in South Africa

Phenotypic and virulence traits ofEscherichia coliandSalmonellastrains isolated from vegetables and fruits from India

Whole Genome Sequencing of Escherichia coli From Store-Bought Produce

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