Phenotypic and virulence traits of<i>Escherichia coli</i>and<i>Salmonella</i>strains isolated from vegetables and fruits from India

Verma, Preeti; Saharan, Vijay Veer; Nimesh, Surendra; Singh, Arvind Pratap

doi:10.1111/jam.13754

Cited by 26 publications

(27 citation statements)

References 45 publications

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“…In the present study, the recovery rate of E. coli (9.2 %) from leafy greens is consistent with other worldwide findings that reported the prevalence of E. coli in fresh produce, including leafy greens, within a range of 5.3-16.7% [10,22,24,40]. However, Salmonella contamination (6.5 %) of leafy greens in the present study ranged between 1.8 and 10%, as reported in previous studies on vegetable samples [22,24,41]. However, little information is available on DEC isolates from fresh produce [42,43], especially leafy greens [40].…”

Section: Discussionsupporting

confidence: 92%

Leafy greens as a potential source of multidrug-resistant diarrhoeagenic Escherichia coli and Salmonella

et al. 2021

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A continued rise in leafy green-linked outbreaks of disease caused by pathogenic Escherichia coli or Salmonella , particularly strains exhibiting multidrug resistance (MDR), has emerged as a major threat to human health and food safety worldwide. Thus, the present study was conducted to examine antimicrobial resistance, including MDR, in diarrhoeagenic E. coli (DEC) and Salmonella isolates obtained from leafy greens from rural and urban areas of India. Of the collected samples (830), 14.1 and 6.5% yielded 117 E. coli (40 DEC and 77 non-DEC) and 54 Salmonella isolates, respectively. Among the DEC pathotypes, enteroaggregative E. coli was the most prevalent (10.2 %), followed by enteropathogenic E. coli (9.4 %), enteroinvasive E. coli (7.6 %) and enterohemorrhagic E. coli (6.8 %). Antimicrobial susceptibility testing of all bacterial isolates with respect to drugs categorized as critically or highly important in both human and veterinary medicine revealed moderate to high (30–90%) resistance for amoxicillin/clavulanic acid, ampicillin, gentamycin and colistin, but relatively low resistance (>30 %) for ciprofloxacin, trimethoprim/sulfamethoxazole and fosfomycin. Notably, all DEC and more than 90% non-DEC or Salmonella isolates were found to be multidrug-resistant to drugs of both human and animal importance. Overall, the results of the present study suggest that leafy greens are potential reservoirs or sources of multidrug-resistant DEC and Salmonella strains in the rural or urban areas of India.

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Section: Discussionsupporting

confidence: 92%

Leafy greens as a potential source of multidrug-resistant diarrhoeagenic Escherichia coli and Salmonella

et al. 2021

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“…Emergence and spread of multi-drug resistance in bacterial pathogens, globally, constitute a nurturing threat to public health (Laxminarayan et al, 2013). Over the past few years, significant evidences have emerged worldwide showing the origin of MDR in the population of food-borne bacteria of animal agriculture (Doyle, 2015), as well as of fresh produce (Verma, Saharan, Nimesh, & Singh, 2018), which is in concurrence with bacteria of clinical settings (Laxminarayan et al, 2013). In the present study, MDR of different patterns was observed both for gram-negative (E. coli, Salmonella spp.)…”

Section: Discussionmentioning

confidence: 99%

High prevalence of antimicrobial resistance in Escherichia coli , Salmonella spp. and Staphylococcus aureus isolated from fish samples in India

2019

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Antibiotic resistance, driven by inappropriate use of antimicrobial agents for fish farming, is an expanding global health threat. Demand of animal protein for human consumption is entraining at remarkable pace which in turn, upturning the non‐judicial use of life‐saving drugs in these modern animal production practices at an incautious rate. Hence, study was devised to observe the antimicrobial resistance (AMR) pattern of human prominence bacteria, against drugs of critically and highly importance of both human and veterinary medicine, circulating over the fish farming in India. One hundred sixty fish faecal samples were obtained from distinct geographical locales of Rajasthan, India. Overall, 202 isolates comprising of Escherichia coli (81, 51%), Salmonella spp. (72, 45%) and Staphylococcus aureus (49, 31%) were isolated to screen for resistance. Antimicrobial resistance has been analysed using broth microdilution method. In case of E. coli and Salmonella spp., highest resistance (>95%) was observed against streptomycin while for S. aureus, it was observed against trimethoprim, along with high resistance to other antibiotics. Majority of the isolates were found to be resistant to more than three antibiotics implying multi‐drug resistance (>89%) relative to the critical and highly important antibiotics. The high AMR is a reflection of misuse of these agents in the fish farming, which is a concern of serious health issues. Our findings persuade the prudent use of antimicrobial agents in fish farming and other aquaculture settings that may assist in drafting of the new policies for the judicial use of these life‐saving drugs in these sectors.

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“…Table 2 and 3 show rdar morphotype was expressed by 18 (78.3%) isolates of E. coli (SMDS2, SMDS4, WMDS, SMKG1, SMKG2, SMKG3, SMKG4, SMKB2, SMKB4, SMKB5, SMKB8, SMK2, SMK3, SMU2, SMT4, WMSK4, WMSK7 and WMPP2) and rdar morphotypes absent in all Salmonella enterica. Verma et al (2018) reported that rdar morphotype characterized by both expressions of curli fimbriae and cellulose production was identified in 28 isolates (38.3%) of E. coli isolated from fresh vegetables and fruits samples.…”

Section: Colony Morphology Testmentioning

confidence: 99%

“…In another study, a higher percentage of saw morphotype was observed. It was found that 9 isolates (12.3%) of E. coli isolated from fresh vegetables and fruits sample did not bind with congo red dye and declared as negative (Verma et al, 2018).…”

Section: Colony Morphology Testmentioning

confidence: 99%

Genotypic and phenotypic characteristics associated with biofilm formation in Escherichia coli and Salmonella spp. isolated from ulam in Terengganu

Bahri¹,

Abdullah²,

Lani³

et al. 2019

Food Res.

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Nowadays, the foodborne outbreaks associated with fresh produces, including ulam, are increasing worldwide. The biofilm formation or bacterial attachment to plant surface is the initial step towards the contamination in fresh produce. The biofilm phenotype of bacteria grown on congo red agar is termed as red, dry and rough (rdar) morphotype. The binding of congo red dye with both biological proteins and inert surfaces is due to the presence of curli fimbriae and cellulose as the main extracellular components. The objective of this study was to determine the rdar morphotypes, biofilm ability and the role of csgA gene of Escherichia coli and Salmonella spp. isolated from ulam or Malaysian herbs. A total of 29 isolates, including 23 E. coli and 6 Salmonella spp. were analyzed for their ability to produce biofilm by colony morphology test, microtiter plate biofilm assay and qualitative biofilm test (pellicle formation). The presence of the csgA gene of E. coli was identified by PCR, which demonstrated the potential gene that able to produce curli fimbriae. Results revealed that 16 (69.6%) E. coli isolates were categorized as strong biofilm producers, 2 (8.7%) as moderate biofilm producers, 3 (13%) as weak biofilm producers, whereas 2 (8.7%) as negative biofilm producers (did not produce biofilm). While 4 (66.7%) Salmonella spp. isolates were identified as strong biofilm producers, 1 (16.7%) as moderate biofilm producers and 1 (16.7%) as negative biofilm producers. Majority of the E. coli strains (69.6%) were identified as strong biofilm producers and able to express rdar morphotypes. The ability of the of E. coli and Salmonella spp. isolates to form biofilm reveals the ability of these isolates to persist on the fresh vegetables and become hosts for the disease transmission to humans or/and animals.

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Phenotypic and virulence traits ofEscherichia coliandSalmonellastrains isolated from vegetables and fruits from India

Abstract: The present study provides valuable baseline information that E. coli and Salmonella may use plants as an alternative host with significant clinical importance.

Cited by 26 publications

References 45 publications

Leafy greens as a potential source of multidrug-resistant diarrhoeagenic Escherichia coli and Salmonella

Leafy greens as a potential source of multidrug-resistant diarrhoeagenic Escherichia coli and Salmonella

High prevalence of antimicrobial resistance in Escherichia coli , Salmonella spp. and Staphylococcus aureus isolated from fish samples in India

Genotypic and phenotypic characteristics associated with biofilm formation in Escherichia coli and Salmonella spp. isolated from ulam in Terengganu

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