Characterization of an atmospheric pressure air plasma device under different modes of operation and their impact on the liquid chemistry

Abstract

It has been shown that plasma generated in contact with liquid can be tailored to tune the composition of plasma functionalized liquids. For biomedical applications, it is necessary to understand the generation of the plasma treated liquids to modulate the composition and thus the biological response. In this work, two distinct discharge compositions were realized by modifying the location of the ground electrode in a pin-to-liquid plasma system. Through this simple modification to the configurations, the spatiotemporal characteristics of the discharge were significantly affected which, in turn, affected the composition of the generated plasma activated water (PAW). Colorimetric testing of the PAW generated from each system revealed that only one configuration was able to generate PAW with a high concentration of H2O2. Using time-, space-, and wavelength-resolved imaging of excited plasma species [OH, N2 (SPS), N2+ (FNS), and atomic O], the differences in PAW composition were linked to the differences observed in the discharge dynamics between the two configurations.

Cell death induced in glioblastoma cells by Plasma-Activated-Liquids (PAL) is primarily mediated by membrane lipid peroxidation and not ROS influx

“…Briefly, 10 μl of TiOSO4 solution and 100 μl of PAW or calibration curve samples were added to a 96-well microtiter plate. After 10 min incubation at room temperature, absorbance was read on a spectrophotometric plate reader at 405 nm [ 15 ].…”

“…We hypothesised that accumulation of PtNP in cells would allow us to differentiate between the role of extracellular and intracellular ROS in mediating cell damage and cytotoxicity. Therefore, in the current study, we investigate the roles of PtNPs when cancerous U-251 MG cells are exposed to indirect plasma treatment, using a previously reported spark discharge PAL generating device ( Fig 1 ) [ 15 ].…”

Generation of neutral pH high‐strength plasma‐activated water from a pin to water discharge and its bactericidal activity on multidrug‐resistant pathogens

“…Figure 1a shows the schematic of the reactor and the diagnostic setup used throughout the present study. The plasma type used in this study is “Pin‐to‐Water spark discharge.” [ 26,34 ] An inverted funnel enclosed the discharge to enhance the strength of the PAW (discussed later in Section 3.4), which is a novel design intervention of this study. Figure 1b shows the dimension of the plasma enclosure used in this study.…”

Recent Advances and Potential Applications of Atmospheric Pressure Cold Plasma Technology for Sustainable Food Processing

“…Thus, if water is treated with ACP, the species mentioned above and H 2 O 2 can be formed. These species have been detected in the liquid phase of plasma-activated water and have been determined to contain highly oxidizing ability in especially biological systems [58][59][60].…”