Characterization of an antibacterial peptide produced by Brevibacterium linens

Motta, Amanda de Souza da; Brandelli, Adriano

doi:10.1046/j.1365-2672.2002.01490.x

Cited by 159 publications

(87 citation statements)

References 28 publications

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“…Antimicrobial activity was determined essentially as described by Motta and Brandelli (2002). An aliquot of 20 µl of BLS P34 (1600 AU) was applied on surface of BHI agar plates previously inoculated with a swab submerged in suspensions of E. faecalis or S. aureus that corresponded to a 76 GÉSSICA A. COSTA et al…”

Section: Antimicrobial Activity Of Bls P34 Against Planktonic Cells Omentioning

confidence: 99%

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Costa

Rossatto

Medeiros

et al. 2018

An. Acad. Bras. Ciênc.

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The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocinlike substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated with the crystal violet assay and with the reduction of 3-bromide [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium. The BLS P34 promoted a reduction of percentage of adhered microbial cells on the surface, not being able to perform the complete elimination of biofilm formation. The metabolic activity of S. aureus biofilms decreased considerably between 41-95%. However, E. faecalis cells showed up metabolically stimulated. The BLS P34 has the potential antibiofilm for the species S. aureus. Studies suggest more detailed approaches to a better understanding of the interactions between the antimicrobial and bacterial cells within the biofilm structure.

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Section: Antimicrobial Activity Of Bls P34 Against Planktonic Cells Omentioning

confidence: 99%

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Costa

Rossatto

Medeiros

et al. 2018

An. Acad. Bras. Ciênc.

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“…The antibacterial activity was initially tested against Listeria monocytogenes ATCC 7466 by agar disk diffusion assay (21). Aliquots of 24-h culture media were centrifuged at 10,000 g for 10 min, at 4°C.…”

Section: Antibacterial Activitymentioning

confidence: 99%

“…Plates were incubated aerobically at 35°C for 24 h. The diameters of the inhibition zones around the disks were measured. The activity of BLS was defined as the reciprocal of the highest dilution yielding a zone of growth inhibition, and expressed as activity units (AU) per mL (21).…”

Section: Bls Activity Assaymentioning

confidence: 99%

Production of bacteriocin-like substances by lactic acid bacteria isolated from regional ovine cheese

Nespolo¹,

Brandelli²

2010

Braz. J. Microbiol.

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Lactic acid bacteria (LAB) were isolated from ovine milk and cheeses manufactured in the South Region of Brazil. Among 112 bacterial isolates investigated, 59 were chosen through a screening for LAB. Among these 59 strains of LAB, 21% showed antimicrobial, proteolytic and lipolytic activities. Based on this screening, Lactobacillus plantarum LCN 17 and Lactobacillus rhamnosus LCN 43 were selected and tested for the production of bacteriocin-like substances (BLS). The BLS produced by both isolates showed antimicrobial activity against Listeria monocytogenes, whereas that produced by L. plantarum LCN 17 presented higher stability to different temperature, pH and enzyme treatments. These strains present potential for production of BLS, and for use as starter cultures.

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“…Antimicrobial activity was determined essentially as described elsewhere [32]. Briefly, aliquots of 20 µl of purified peptide P34 and nisin were applied on cellulose disks (6 mm) on BHI agar plates previously inoculated with indicator strain suspension (L. monocytogenes ATCC 7644 or L. seeligeri AC 82/4), which corresponded to 0.5 McFarland turbidity standard solution.…”

Section: Detection Of Antimicrobial Activitymentioning

confidence: 99%

Influence of Peptide P34 on Gene Expression of Listeria Monocytogenes and Listeria Seelegeri

Vaucher

Giongo²,

Estivales

et al. 2016

Int J Pharm Pharm Sci

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Objective: Investigate the influence of the antimicrobial peptides P34 and nisin on the expression of genes associated with components of the cell surface of Listeria monocytogenes and Listeria seeligeri.Methods: Antimicrobial activity was determined by addition of peptide P34 and nisin (12.5 µg/ml) onto Brain Heart Infusion agar (BHI) plates previously inoculated with indicator strains (L. monocytogenes ATCC 7644 or L. seeligeri AC 82/4) after incubation for 24 h at 37 °C or 240 h at 4 °C. Ribonucleic acid (RNA) was directly extracted from bacterial colonies at the border of the inhibition zones, and the expression levels of genes Dalanine-D-alanyl carrier protein ligase (dltA), putative phospholipid lysinylation (Imo 1695) and EIIAB Man Results: A non-significant increase in the levels of transcription of genes dltA, Imo1695 and mptA was observed for L. monocytogenes treated with peptide P34 or nisin. Both peptides caused a similar decrease in dltA gene expression in L. seeligeri. The expression of gene Imo1695 significantly decreased (about 2000-fold) after treatment with the peptide P34 at 37 °C, while at 4 °C a reduction of 12-fold and 5-fold was detected for P34 and nisin, respectively. A significant decrease in mptA gene expression was observed by exposition to peptide P34 (31.872-fold) and nisin (16.047-fold) for 24 h at 37 °C.of mannose-specific PTS (mptA) were determined using real-time PCR. Conclusion:The results suggest that both peptide P34 and nisin influence the expression of genes related with the cell-surface/cell-membrane structure of L. seeligeri and in lesser extent L. monocytogenes.

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Characterization of an antibacterial peptide produced by Brevibacterium linens

Cited by 159 publications

References 28 publications

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Production of bacteriocin-like substances by lactic acid bacteria isolated from regional ovine cheese

Influence of Peptide P34 on Gene Expression of Listeria Monocytogenes and Listeria Seelegeri

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