Abstract:Yersinia pseudotuberculosis which were screened out depending on autoagglutination and Ca2+ dependency, were examined for their production of hemagglutinin (HA), and its purification and characterization were performed . The HA with a broad reactivity with various mammalian erythrocytes was recovered from the cultui e supernatant of these strains grown at 37 C but not 25 C. HAs from two strains, R148R and T1040, were purified by salt precipitation, gel filtration and anion-exchange chromatography by HPLC. Both… Show more
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