Identification of a novel adhesin-like glycoprotein from Mycoplasma hyopneumoniae

Chen, Jia‐Rong; Lin, Jianfeng; Weng, Chung-Nan; Lai, Shiow-Suey

doi:10.1016/s0378-1135(98)00207-7

Cited by 9 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the antiadhesive action of episialin in human endometrial-blastocyst attachment appears due to episialin glycosylation, and the antiadhesive activity is reduced by treatment with O-sialoglycoprotein endopeptidase (Chervenak and Illsley, 2000). Many pathogenic bacteria, including members of class Mollicutes (Alvarez et al, 2003;Berg et al, 2001;Chen et al, 1998;Razin, 1999;Roberts et al, 1989), have evolved mechanisms for attachment to host cell surfaces. In some cases, binding to host cells involves bacterial lectins that recognize and bind to sialylated cell-surface glycoproteins (Hirmo et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Folate Biosynthesis Pseudogenes, ΨfolPand ΨfolK, and an O-Sialoglycoprotein Endopeptidase Gene Homolog in the Phytoplasma Genome

Davis

Jomantienė

Zhao

et al. 2003

DNA and Cell Biology

View full text Add to dashboard Cite

Phytoplasmas are wall-less phytopathogenic prokaryotes of small genome sizes that are obligate parasites of insect vectors and plant hosts. We have cloned a clover phyllody (CPh) phytoplasma DNA locus containing five potential coding sequences. Two were identified as pseudogenes (PsifolP and PsifolK) homologous to folP and folK genes, which encode dihydropteroate synthase (DHPS) and 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK), respectively, in other bacteria. Evolution of the phytoplasma presumably involved loss of functions through the formation of these and other pseudogenes during adaptation to obligate parasitism. The findings suggest that the phytoplasma lacks capacity for de novo folate biosynthesis and possesses a transport system for absorption of preformed folate from host cells. The PsifolP-PsifolK region was flanked by three open reading frames (ORFs) encoding a DegV family protein, a hypothetical protein with a P60-like lipoprotein domain homologous with the P60-like Mycoplasma hominis protein, and a glycoprotease (Gcp) protein that possibly functions as a host adaptation or virulence factor.

show abstract

Section: Discussionmentioning

confidence: 99%

Folate Biosynthesis Pseudogenes, ΨfolPand ΨfolK, and an O-Sialoglycoprotein Endopeptidase Gene Homolog in the Phytoplasma Genome

Davis

Jomantienė

Zhao

et al. 2003

DNA and Cell Biology

View full text Add to dashboard Cite

show abstract

“…Zhang et al (1995) identified the P97 protein to be a ciliary adhesin. Other adhesins may include a glycoprotein of 110 kDa (Chen et al, 1998), a P159 protein that is post-transitionally cleaved in proteins of 27, 51 and 110 kDa (Burnett et al, 2006) and a 146 kDa protein (Stakenborg et al, 2006). M. hyopneumoniae affects the mucosal clearance system by disrupting the cilia on the epithelial surface and, additionally, the organism modulates the immune system of the respiratory tract (Thacker, 2006).…”

Section: Introductionmentioning

confidence: 99%

Control of Mycoplasma hyopneumoniae infections in pigs

Maes

Segalés

Meyns

et al. 2008

Veterinary Microbiology

349

311

View full text Add to dashboard Cite

Mycoplasma hyopneumoniae, the primary pathogen of enzootic pneumonia, occurs worldwide and causes major economic losses to the pig industry. The organism adheres to and damages the ciliated epithelium of the respiratory tract. Affected pigs show chronic coughing, are more susceptible to other respiratory infections and have a reduced performance. Control of the disease can be accomplished in a number of ways. First, management practices and housing conditions in the herd should be optimized. These include all-in/all-out production, limiting factors that may destabilize herd immunity, maintaining optimal stocking densities, prevention of other respiratory diseases, and optimal housing and climatic conditions. Strategic medication with antimicrobials active against M. hyopneumoniae and, preferably, also against major secondary bacteria may be useful during periods when the pigs are at risk for respiratory disease. Finally, commercial bacterins are widely used to control M. hyopneumoniae infections. The main effects of vaccination include less clinical symptoms, lung lesions and medication use, and improved performance. However, bacterins provide only partial protection and do not prevent colonization of the organism. Different vaccination strategies (timing of vaccination, vaccination of sows, vaccination combined with antimicrobial medication) can be used, depending on the type of herd, the production system and management practices, the infection pattern and the preferences of the pig producer. Research on new vaccines is actively occurring, including aerosol and feed-based vaccines as well as subunit and DNA vaccines. Eradication of the infection at herd level based on age-segregation and medication is possible, but there is a permanent risk for re-infections.

show abstract

“…The study of virulence factors in M. hyopneumoniae has been centered in the characterization of adhesion mediating molecules, especially the P97 adhesin (see, for example, Zhang et al, 1994;Chen et al, 1998;Minion, 1998a 1998b;Djordjevic et al, 2004). However, the mechanisms of M. hyopneumoniae pathogenicity allow to predict the existence of several other classes of not yet identified virulence factors, including genes/proteins involved with secretion and/or traffic between host and pathogen cells, or with evasion and/or modulation of the host immune system.…”

Section: Introductionmentioning

confidence: 99%

A preliminary survey of M. hyopneumoniae virulence factors based on comparative genomic analysis

Ferreira

Castro

2007

Genet. Mol. Biol.

View full text Add to dashboard Cite

Mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia (PEP), a major problem for the pig industry. The mechanisms of M. hyopneumoniae pathogenicity allow to predict the existence of several classes of virulence factors, whose study has been essentially restricted to the characterization of adhesion-related and major antigenic proteins. The now available complete sequences of the genomes of two pathogenic and one non-pathogenic strain of M. hyopneumoniae allowed to use a comparative genomics approach to putatively identify virulence genes. In this preliminary survey, we were able to identify 118 CDSs encoding putative virulence factors, based on specific criteria ranging from predicted cell surface location or variation between strains to previous functional studies showing antigenicity or involvement in host-pathogen interaction. This survey is expected to serve as a first step towards the functional characterization of new virulence genes/proteins that will be important not only for a better comprehension of M. hyopneumoniae biology, but also for the development of new and improved protocols for PEP vaccination, diagnosis and treatment.

show abstract

Identification of a novel adhesin-like glycoprotein from Mycoplasma hyopneumoniae

Cited by 9 publications

References 33 publications

Folate Biosynthesis Pseudogenes, ΨfolPand ΨfolK, and an O-Sialoglycoprotein Endopeptidase Gene Homolog in the Phytoplasma Genome

Folate Biosynthesis Pseudogenes, ΨfolPand ΨfolK, and an O-Sialoglycoprotein Endopeptidase Gene Homolog in the Phytoplasma Genome

Control of Mycoplasma hyopneumoniae infections in pigs

A preliminary survey of M. hyopneumoniae virulence factors based on comparative genomic analysis

Contact Info

Product

Resources

About