Characterization of ammonia‐assimilating bacteria in a lagoon for wastewater from a paddock of dairy cattle

Nonaka

J Ind Microbiol Biotechnol

et al. 2006

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We isolated ammonia-assimilating microorganisms from the livestock manure treatment systems and evaluated their ammonia-assimilating ability. Many isolates utilized ammonia at high rates when they were purely cultivated in a nitrogen-limited medium to which sterilized lagoon extract had been added. Some isolates that were immobilized in polyvinyl alcohol (PVA) utilized ammonia present in the media containing viable lagoon microorganisms. Staining with 4',6'-diamidino-2-phenylindole (DAPI) indicated that the immobilized high ammonia-assimilating isolates grew dominantly within the PVA beads. High ammonia-assimilating isolates in the mixed culture containing viable lagoon microorganisms were identified as Pseudomonas spp. and member of Rhizobiaceae species by partial sequencing of the 16S ribosomal DNA.

“…We have already isolated a total of 34 strains from the lagoon [15]. Nine of them were used in this study.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Culture Mediummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ammonia removal from livestock wastewater by ammonia-assimilating microorganisms immobilized in polyvinyl alcohol

Nonaka

J Ind Microbiol Biotechnol

et al. 2006

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Journal of the Air & Waste Management Association

“…Contamination of the medium with environmental microorganisms did not inhibit the H 2 S removal efficiency. Sasaki et al (2002), reported that ammonia assimilation by strain L2510 (99% 16S rRNA gene sequence similarity to Buttiauxella agrestis) decreased by 80% in the presence of microorganisms from lagoon water. As Buttiauxella spp.…”

Section: Discussionmentioning

confidence: 99%

Middle-thermophilic sulfur-oxidizing bacteriaThiomonassp. RAN5 strain for hydrogen sulfide removal

Asano

Hirooka

Nakai

2011

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Hydrogen sulfide (H 2 S) is one of the most toxic and offensively odorous gases and is generated in anaerobic bioreactors. A middle-thermophilic sulfur-oxidizing bacterium (SOB), Thiomonas sp. strain RAN5, was isolated and applied for H 2 S removal from both artificial and anaerobically digested gas. When a bioreactor containing medium inoculated with RAN5 was aerated continuously with artificial gas (containing 100 ppm H 2 S) at 45 C for 156 hr, the H 2 S concentration in the vented gas was reduced by 99%. This was not affected by the presence of other microbes in the bioreactor. The H 2 S removal efficiency of the RAN5 bioreactor for anaerobically digested gas was greater than 99% at influent H 2 S concentrations ranging from 2 to 1800 ppm; the efficiency decreased to 90% at influent H 2 S concentrations greater than 2000 ppm. Thiomonas sp. strain RAN5 cannot survive at room temperature, and thus its leakage from a wastewater treatment plant would not damage sewage systems. These data suggest that Thiomonas sp. strain RAN5 may be a useful microorganism for H 2 S removal.Implications: The hydrogen sulfide removal efficiency of the RAN5 bioreactor was greater than 99% over the long term, for influent H 2 S concentrations of up to 2000 ppm. Removal was not affected by the presence of other microbes. Leakage of strain RAN5 would not damage sewage systems.

A survey of ammonia-assimilating micro-organisms in cattle manure composting

et al. 2005

Aims: To evaluate the ammonia-assimilating abilities of micro-organisms isolated from cattle manure composting processes and to determine the distribution of cultivable species of ammonia-assimilating micro-organisms in microbial communities during the composting processes. Methods and Results: Compost samples were collected from four stages of treatment. Trypto soya agar was used for the isolation of ammonia-assimilating aerobes. Many of the isolates showed high ammonia-assimilating ability in a medium containing basal components and a compost extract. Partial 16S ribosomal DNA sequencing showed that the cultivable species of highly efficient ammonia-assimilating isolates changed during the composting process. The community structure of micro-organisms and actinomycetes was analysed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Two species of actinomycetes identified by PCR-DGGE coincided with those found among the cultured isolates. Conclusions: Ammonia-assimilating micro-organisms obtained by the cultivation method were not predominant in the microbial community during the composting process: however certain cultured actinomycetes were members of predominant species in the actinomycetes community. Significance and Impact of the Study: Ammonia assimilation by micro-organisms is one of the important mechanisms for ammonia retention in the composting process. Cultivable actinomycetes are a means for preventing ammonia emission from the composting process.