Middle-thermophilic sulfur-oxidizing bacteriaThiomonassp. RAN5 strain for hydrogen sulfide removal

Abstract

Hydrogen sulfide (H 2 S) is one of the most toxic and offensively odorous gases and is generated in anaerobic bioreactors. A middle-thermophilic sulfur-oxidizing bacterium (SOB), Thiomonas sp. strain RAN5, was isolated and applied for H 2 S removal from both artificial and anaerobically digested gas. When a bioreactor containing medium inoculated with RAN5 was aerated continuously with artificial gas (containing 100 ppm H 2 S) at 45 C for 156 hr, the H 2 S concentration in the vented gas was reduced by 99%. This was not affected by the presence of other microbes in the bioreactor. The H 2 S removal efficiency of the RAN5 bioreactor for anaerobically digested gas was greater than 99% at influent H 2 S concentrations ranging from 2 to 1800 ppm; the efficiency decreased to 90% at influent H 2 S concentrations greater than 2000 ppm. Thiomonas sp. strain RAN5 cannot survive at room temperature, and thus its leakage from a wastewater treatment plant would not damage sewage systems. These data suggest that Thiomonas sp. strain RAN5 may be a useful microorganism for H 2 S removal.Implications: The hydrogen sulfide removal efficiency of the RAN5 bioreactor was greater than 99% over the long term, for influent H 2 S concentrations of up to 2000 ppm. Removal was not affected by the presence of other microbes. Leakage of strain RAN5 would not damage sewage systems.

Spatial Metagenomics of Three Geothermal Sites in Pisciarelli Hot Spring Focusing on the Biochemical Resources of the Microbial Consortia

“…The analysis of the relative abundances of bacteria present in the microbial community of Site B ( Figure 5 B) allowed us to identify the hyperthermophiles Thermoanaerobacter (26%) and Caldanaerobacter (9%), the thermophile Thermoanaerobacterium (10%), and Acidithiobacillus , which, unlike in Site A, was much less abundant here (4%). It is important to note that one of the most abundant bacterial genus present in Site B was Thiomonas (14%), which, although generally grouping mesophilic species, also includes moderately thermophilic species identified in geothermal sources at ~45 °C and able to grow at temperatures up to 50 °C and in the pH range 4.0–7.0 [ 29 , 30 , 31 ].…”

Biofiltration of H 2 S in air—Experimental comparisons of original packing materials and modeling

“…In the case of H 2 S biodegradation, sulfur oxidizing bacteria (SOB) are responsible for removing H 2 S in aerobic conditions. For their maintenance and growth, SOB use H 2 S as a source of energy and CO 2 as the main source of carbon [1,2]. Bacteria from the genus Thiobacillus are responsible for the oxidation of H 2 S to sulfate and/or elemental sulfur according to the operating conditions [3,4].…”

Biological technologies for the removal of sulfur containing compounds from waste streams: bioreactors and microbial characteristics

“…The development of immobilized thermophilic desulfurization bacteria in bioreactors for thermophilic operation creates more rapid and economical treatment processes (Asano et al 2012). To treat hot gases containing hydrogen sulfide, Ryu et al (2009) used a biofilter filled with porous particles inoculated with Bacillus sp.…”