Ammonia removal from livestock wastewater by ammonia-assimilating microorganisms immobilized in polyvinyl alcohol

Abstract

We isolated ammonia-assimilating microorganisms from the livestock manure treatment systems and evaluated their ammonia-assimilating ability. Many isolates utilized ammonia at high rates when they were purely cultivated in a nitrogen-limited medium to which sterilized lagoon extract had been added. Some isolates that were immobilized in polyvinyl alcohol (PVA) utilized ammonia present in the media containing viable lagoon microorganisms. Staining with 4',6'-diamidino-2-phenylindole (DAPI) indicated that the immobilized high ammonia-assimilating isolates grew dominantly within the PVA beads. High ammonia-assimilating isolates in the mixed culture containing viable lagoon microorganisms were identified as Pseudomonas spp. and member of Rhizobiaceae species by partial sequencing of the 16S ribosomal DNA.

Degradation of cefdinir from pharmaceutical wastewater using immobilized Candida sp. SMN04 and biofilm formed on gravels

“…Pseudomonas putida and Pseudomonas fluorescens (Krishnan et al, 2012), Bacillus and Bacteriods (Wagner et al, 2011) and Ustilago sp. SMN03 (Selvi and Das, 2014a) which are capable of using cephalosporin derivatives as the sole source of carbon and energy. But free microbial cells have certain limitations viz., small particle size, possible clogging and low mechanical strength of the biomass (Godjevargova et al, 2004).…”

Efficient total nitrogen removal from wastewater by Paracoccus denitrificans DYTN‐1

“…Therefore, immobilization could be a useful option in the bioremediation of toxic organic solvents. Higher volumetric reaction rates may be obtained with immobilized cells as a result of their higher local cell concentration or altered cell permeability, and therefore immobilized cells have been used to remove numerous toxic chemicals from industrial effluents (Bettmann and Rehm ; Manohar and Karegoudar ; Manohar et al ; Chen et al ; Patil et al ; Sasaki et al ). Immobilized cells also have applications in the food industry (Rosenberg et al ; Rebroš et al ; Grosová et al ; Hronsk et al ), bio‐transformations (Bruss et al ; Wilson et al ; Kubáč et al ; Vejvoda et al ) and bioethanol production (Rebros et al ; Rebroš et al ).…”

“…Therefore, immobilization could be a useful option in the bioremediation of toxic organic solvents. Higher volumetric reaction rates may be obtained with immobilized cells as a result of their higher local cell concentration or altered cell permeability, and therefore immobilized cells have been used to remove numerous toxic chemicals from industrial effluents (Bettmann and Rehm 1984;Manohar and Karegoudar 1998;Manohar et al 2001;Chen et al 2002;Patil et al 2006;Sasaki et al 2007). In this study, we investigated the optimal conditions for P. denitrificans to remove total nitrogen (TN) from wastewater, and the effect of SA complexation for immobilizing P. denitrificans cells with several different divalent metal cations to form a hydrogel during sewage treatment.…”

Biodegradation of Linear Alkylbenzene Sulfonate (LAS) by Immobilized <i>Pseudomonas</i> sp.

“…PVA is widely used for waste water treatment processes. PVA gels are easily prepared from aqueous PVA solutions and have the characteristics of high porosity and stability [7].…”

“…The use of immobilised microorganisms for the production of various useful chemicals and biodegradation of xenobiotics is an important aspect of microbial technology [6] [7]. Alginate immobilized cells are easily prepared and are more stable than free cells over a long period.…”