Characterization of a thermophilic cellulase from Geobacillus sp. HTA426, an efficient cellulase-producer on alkali pretreated of lignocellulosic biomass

Potprommanee, Laddawan; Wang, Xiaoqin; Han, Yang‐Su; Nyobe, Didonc; Peng, Yen-Ping; Huang, Qing; Liu, Jingyong; Liao, Yu-Ling; Chang, Ken-Lin

doi:10.1371/journal.pone.0175004

Cited by 67 publications

(36 citation statements)

References 52 publications

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“…Based on the HCR ratio, many potent cellulolytic bacteria were previously screened from various regions, such as Geobacillus sp. from a hot spring and Paenibacillus lautus BHU3 from a landfill site [18,19]. Similarly, PX12 was considered as a good cellulolytic bacterium and was used for further study.…”

Section: Identification and Cellulose-degrading Potential Of Bacillusmentioning

confidence: 99%

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Lü

Zheng

et al. 2020

Biotechnol Biofuels

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Background: Bamboo, a lignocellulosic feedstock, is considered as a potentially excellent raw material and evaluated for lignocellulose degradation and bioethanol production, with a focus on using physical and chemical pre-treatment. However, studies reporting the biodegradation of bamboo lignocellulose using microbes such as bacteria and fungi are scarce. Results:In the present study, Bacillus velezensis LC1 was isolated from Cyrtotrachelus buqueti, in which the symbiotic bacteria exhibited lignocellulose degradation ability and cellulase activities. We performed genome sequencing of B. velezensis LC1, which has a 3929,782-bp ring chromosome and 46.5% GC content. The total gene length was 3,502,596 bp using gene prediction, and the GC contents were 47.29% and 40.04% in the gene and intergene regions, respectively. The genome contains 4018 coding DNA sequences, and all have been assigned predicted functions. Carbohydrate-active enzyme annotation identified 136 genes annotated to CAZy families, including GH, GTs, CEs, PLs, AAs and CBMs. Genes involved in lignocellulose degradation were identified. After a 6-day treatment, the bamboo shoot cellulose degradation efficiency reached 39.32%, and the hydrolysate was subjected to ethanol fermentation with Saccharomyces cerevisiae and Escherichia coli KO11, yielding 7.2 g/L of ethanol at 96 h. Conclusions:These findings provide an insight for B. velezensis strains in converting lignocellulose into ethanol. B. velezensis LC1, a symbiotic bacteria, can potentially degrade bamboo lignocellulose components and further transformation to ethanol, and expand the bamboo lignocellulosic bioethanol production.

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Section: Identification and Cellulose-degrading Potential Of Bacillusmentioning

confidence: 99%

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Lü

Zheng

et al. 2020

Biotechnol Biofuels

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“…Cellulose, a homopolymer of D-glucose units linked by β-1, 4 bonds, is the most abundant organic polymer on the earth [1][2][3]. It is an important structural component of the primary cell wall of green plants and potential renewable resources in the nature [4].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Cellulase-producing Bacteria from Sugar Industry Waste

Islam¹

2019

AJBIO

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Cellulases are inducible enzymes that are synthesized by a large number of microorganisms during their growth on cellulosic materials. This study focuses on the isolation and screening of cellulase-producing bacteria from sugar industry waste (molasses) and characterization by morphological and biochemical analysis. Further, purification of cellulase was carried by ammonium sulfate precipitation and followed by column chromatography and molecular weight determined by SDS-PAGE. The isolated bacterial strains were grown on carboxymethyl cellulose (CMC) agar plate at various optimum conditions like pH, temperature, incubation period, carbon and nitrogen sources and substrate concentration. Three isolated strains showed clear hydrolyzing zone on agar plates containing CMC agar after Congo-red staining were identified as cellulase-producing bacteria. Based on cultural, morphological, and biochemical characteristics, the isolated strains were identified as Paenibacillus sp., Aeromonas sp., and Bacillus sp. Among the isolated strains, Paenibacillus sp. showed the capability for highest cellulase production (0.89 µmol ml-1 min-1) at optimal pH 7.0 and 40°C temperature on 24 hour of the incubation period at 1% CMC substrate concentration and was selected for further cellulase purification. In the final step of cellulase purification, the specific activity, purification fold and recovery were 1720 U/mg, 9.74 and 35.6%, respectively. The molecular weight of the purified enzyme was determined 66.9 kDa and the enzyme showed a high specificity to CMC substrate. The bacterial strains present in molasses have the potential for cellulase production. Substrate specificity of the purified cellulase indicates it to be an endo-β-1, 4-glucanase. The cellulase produced from the selected strain may benefit for industrial application.

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“…Por esse motivo é essencial a determinação do período de crescimento bacteriano em que a enzima é produzida. Geobacillus (Potprommanee L et al, 2017), 60 h para Paenibacillus (Liang Y-L et al, 2014), e 48 h e 60 h para o mesmo isolado de Bacillus sp. quando incubado na presença ou ausência de agitação (Goyal V et al, 2014).…”

Section: Discussionunclassified

Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

Devaraj

Aathika

Periyasamy

et al. 2018

Natural Product Research

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A strain of Bacillus amyloliquefaciens KUB29 was identified by 16S ribosomal RNA sequencing (Genbank: MF772779.1). Production of thermostable protease, amylase and lipase were done by the isolated strain. The produced enzymes were partially purified by ammonium precipitation followed by dialysis process. Protease and lipase enzymes are effectively used in bio-oil extraction from proteinaceous sample followed by transesterification to produce methyl ester. Amylase enzyme is widely used in food and laundry industry. The produced enzymes are active at thermophilic condition of 55 °C. Use of these enzymes in biofuel production process will make the process cleaner and greener.

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Characterization of a thermophilic cellulase from Geobacillus sp. HTA426, an efficient cellulase-producer on alkali pretreated of lignocellulosic biomass

Cited by 67 publications

References 52 publications

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Genome sequencing of gut symbiotic Bacillus velezensis LC1 for bioethanol production from bamboo shoots

Isolation and Characterization of Cellulase-producing Bacteria from Sugar Industry Waste

Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

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