Characterization of a proteinaceous adhesin of Staphylococcus epidermidis which mediates attachment to polystyrene

Timmerman, C. P.; Fleer, A.; Besnier, Jean-Marc; Graaf, Loek de; Cremers, Fons; Verhoef, J.

doi:10.1128/iai.59.11.4187-4192.1991

Cited by 106 publications

(27 citation statements)

References 32 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecules with adhesive function can occur not only on the bacterial capsule, but also on the cell wall and membrane. [19][20][21] It has been demonstrated that the bacterial cell frequently has various adherence molecules, or ''adhesins,'' and that it can express one adhesin or the other, according to different situations. Fletcher and Marshall 22 noted that with proteolytic enzyme treatment, Pseudomonas NCMB 2021 can be detached from polystyrene surfaces but not from glass, thus suggesting that different adherence molecules are responsible for adherence to different materials.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of bacterial adherence to a high‐water‐content polymer by a water‐soluble, nonsteroidal, anti‐inflammatory drug

Arciola

Montanaro

Caramazza

et al. 1998

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Deposition and aggregation of lachrymal proteins on the contact lens surface can promote bacterial adherence. Lysozyme is the major tear protein and is also mainly responsible for the formation of protein deposits on contact lenses. Nonsteroidal anti-inflammatory drugs (NSAID) prevent protein aggregation. The effect of a watersoluble NSAID drug on bacterial adherence to high-watercontent/ionic disposable contact lenses was examined in a radiolabeling study. Dose-related inhibition of adherence of Staphylococcus aureus, S. epidermidis, and Pseudomonas aeruginosa on both pretreated lenses and after adding the drug to the medium was investigated. When the drug was added to the media, maximal inhibition of S. aureus adherence was observed in trypticase soy broth (59-98% at the lower and higher drug concentrations, respectively); inhibition progressively decreased in calf aqueous humor (48-75%), lysozyme (34-63%), and saline (12-20%) solutions. Inhibition of adherence varied with the three bacterial species; it was maximal with S. aureus, intermediate with S. epidermidis, and minimal with P. aeruginosa. When lenses were pretreated with the drug, consistent, and even higher, inhibitory effects were observed. The results suggest that water-soluble NSAIDs could be used in preventive treatments for conjunctivae and corneal infections in contact lens wearers, and may provide a clue as to which compounds might inhibit protein interaction and bacterial adhesion.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition of bacterial adherence to a high‐water‐content polymer by a water‐soluble, nonsteroidal, anti‐inflammatory drug

Arciola

Montanaro

Caramazza

et al. 1998

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

show abstract

“…Extracytoplasmic proteins, specifically those proteins on the surface of the bacterial cell, are thought to be important for bacteria to attach to abiotic substrates (Timmerman et al, 1991;Dufrene et al, 1996). To address this point in our system, we determined the effect of treatment with a protease, Pronase E (added at inoculation of cells into microtitre dish wells), on the formation of biofilms using the rapid attachment assay.…”

Section: Extracytoplasmic Proteins Participate In Biofilm Formationmentioning

confidence: 99%

Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis

O’Toole

Kolter

1998

Molecular Microbiology

2,258

1,795

View full text Add to dashboard Cite

SummaryPopulations of surface-attached microorganisms comprising either single or multiple species are commonly referred to as biofilms. Using a simple assay for the initiation of biofilm formation (e.g. attachment to an abiotic surface) by Pseudomonas fluorescens strain WCS365, we have shown that: (i) P. fluorescens can form biofilms on an abiotic surface when grown on a range of nutrients; (ii) protein synthesis is required for the early events of biofilm formation; (iii) one (or more) extracytoplasmic protein plays a role in interactions with an abiotic surface; (iv) the osmolarity of the medium affects the ability of the cell to form biofilms. We have isolated transposon mutants defective for the initiation of biofilm formation, which we term surface attachment defective (sad ). Molecular analysis of the sad mutants revealed that the ClpP protein (a component of the cytoplasmic Clp protease) participates in biofilm formation in this organism. Our genetic analyses suggest that biofilm formation can proceed via multiple, convergent signalling pathways, which are regulated by various environmental signals. Finally, of the 24 sad mutants analysed in this study, only three had defects in genes of known function. This result suggests that our screen is uncovering novel aspects of bacterial physiology.

show abstract

“…The first stage of biofilm formation under experimental conditions involves attachment to a plastic surface. Different factors are proposed to play a role in primary attachment, such as cell surface hydrophobicity (Hogt et a/., 1986;Ludwicka et a/., 1984;Pascual et a/., 19861, a 220 kDa proteinaceous adhesin (Timmerman et a/., 1991), or the capsular polysaccharide/adhesin (Tojo et al, 1988;Muller et a/., 1993). We isolated a transposon-insertion mutant of S. epidermidis that is affected in polystyrene adherence and has a decreased surface hydrophobicity.…”

Section: Introductionmentioning

confidence: 99%

Molecular basis of intercellular adhesion in the biofilm‐forming Staphylococcus epidermidis

Heilmann

Schweitzer

Gerke

et al. 1996

Molecular Microbiology

843

681

View full text Add to dashboard Cite

The Staphylococcus epidermidis genes icaABC are involved in the synthesis of the polysaccharide intercellular adhesin (PIA), which is located mainly on the cell surface, as shown by immunofluorescence studies with PIA-specific antiserum. PIA was shown to be a linear beta-1,6-linked glucosaminoglycan composed of at least 130 2-deoxy-2-amino-D-glucopyranosyl residues of which 80-85% are N-acetylated, the rest being non-N-acetylated and positively charged. A transposon insertion in the icaABC gene cluster (ica, intercellular adhesion) led to the loss of several traits, such as the ability to form a biofilm on a polystyrene surface, cell aggregation, and PIA production. The mutant could be complemented by transformation with the icaABC-carrying plasmid pCN27. Transfer of pCN27 into the heterologous host Staphylococcus carnosus led to the formation of large cell aggregates, the formation of a biofilm on a glass surface, and PIA expression. The nucleotide sequence of icaABC suggests that the three genes are organized in an operon and that they are co-transcribed from the mapped icaA promoter. IcaA contains four potential transmembrane helices, indicative of a membrane location. The deduced IcaA sequence shows similarity to those of polysaccharide-polymerizing enzymes, the most pronounced being with a Rhizobium meliloti N-acetylglucosaminyltransferase involved in lipo-chitin biosynthesis (22.5% overall identity and 37.4% overall similarity). This similarity suggests that IcaA has N-acetylglucosaminyltransferase activity in the formation of the beta-1, 6-linked N-acetyl-D-glucosaminyl polymer. IcaB is secreted into the medium and contains a typical signal peptide. IcaC is hydrophobic and contains six predicted transmembrane helices distributed over its entire length, typical for an integral membrane protein. Neither IcaB nor IcaC shares similarity with known proteins, and their function is unknown. Inactivation of icaA, icaB, or icaC in pCN27 led to the complete loss of the intercellular adhesion phenotype in S. carnosus, suggesting that all three genes are involved in intercellular adhesion, PIA expression, and translocation.

show abstract

Characterization of a proteinaceous adhesin of Staphylococcus epidermidis which mediates attachment to polystyrene

Cited by 106 publications

References 32 publications

Inhibition of bacterial adherence to a high‐water‐content polymer by a water‐soluble, nonsteroidal, anti‐inflammatory drug

Inhibition of bacterial adherence to a high‐water‐content polymer by a water‐soluble, nonsteroidal, anti‐inflammatory drug

Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis

Molecular basis of intercellular adhesion in the biofilm‐forming Staphylococcus epidermidis

Contact Info

Product

Resources

About