Inhibition of bacterial adherence to a high‐water‐content polymer by a water‐soluble, nonsteroidal, anti‐inflammatory drug

Abstract

Deposition and aggregation of lachrymal proteins on the contact lens surface can promote bacterial adherence. Lysozyme is the major tear protein and is also mainly responsible for the formation of protein deposits on contact lenses. Nonsteroidal anti-inflammatory drugs (NSAID) prevent protein aggregation. The effect of a watersoluble NSAID drug on bacterial adherence to high-watercontent/ionic disposable contact lenses was examined in a radiolabeling study. Dose-related inhibition of adherence of Staphylococcus aureus, S. epidermidis, and Pseudomonas aeruginosa on both pretreated lenses and after adding the drug to the medium was investigated. When the drug was added to the media, maximal inhibition of S. aureus adherence was observed in trypticase soy broth (59-98% at the lower and higher drug concentrations, respectively); inhibition progressively decreased in calf aqueous humor (48-75%), lysozyme (34-63%), and saline (12-20%) solutions. Inhibition of adherence varied with the three bacterial species; it was maximal with S. aureus, intermediate with S. epidermidis, and minimal with P. aeruginosa. When lenses were pretreated with the drug, consistent, and even higher, inhibitory effects were observed. The results suggest that water-soluble NSAIDs could be used in preventive treatments for conjunctivae and corneal infections in contact lens wearers, and may provide a clue as to which compounds might inhibit protein interaction and bacterial adhesion.

Detection of icaA and icaD loci by polymerase chain reaction and biofilm formation by Staphylococcus epidermidis isolated from dialysate and needles in a dialysis unit

“…9 Elucidation of the adhesive mechanisms of clinical strains may help in developing antiadhesive coatings or anti-adhesin drugs. 18,19 The production of PIA by this species is mediated by the ica operon.…”

Salmonella biofilms: An overview on occurrence, structure, regulation and eradication

“…Salicylates and other nonsteroidal anti-inflammatory drugs are known to prevent bacterial adhesion on medical devices (Arciola, Montanaro, Caramazza, Sassoli, & Cavedagna, 1998). Rosenberg et al found that the S. Typhimurium strain MAE52, which normally forms pellicles at the air-liquid interface, does not form pellicles when it was grown in growth medium containing a glass coverslip coated with a salicylate-based poly(anhdride ester) from which salicylic acid is released via surface erosion, while the planktonic growth of the bacteria is not reduced (Rosenberg, Carbone, Römling, Uhrich, & Chikindas, 2008).…”

Application of Scanning Electron Microscopy for the Morphological Study of Biofilm in Medical Devices

“…Coating the catheter with acetylsalicylic acid or sodium salicylate reduces or inhibits microbial adherence, Bandazac lysine (non steroidal anti-inflammatory) was found to prevented the adherence of bacteria to contact lenses (Arciola et al, 1998).…”