Characterization of a novel β-glucosidase from a Stachybotrys strain

Amouri, Bahia; Gargouri, Ali

doi:10.1016/j.bej.2006.09.022

Cited by 90 publications

(51 citation statements)

References 15 publications

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“…The molecular weight of the subunits was similar to a ginsenoside-hydrolyzing -glucosidase isolated from China white jade snail, 15) but it was higher than most previously reported -glucosidases. 13,14,24,25) Moreover, the reported -glucosidases are, to our knowledge, monomers, dimmers, and tetramers. No trimericglucosidase has been identified.…”

Section: Discussionmentioning

confidence: 99%

Purification Method Improvement and Characterization of a Novel Ginsenoside-Hydrolyzing β-Glucosidase fromPaecilomyces Bainiersp. 229

Yan

Zhou

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

Purification Method Improvement and Characterization of a Novel Ginsenoside-Hydrolyzing β-Glucosidase fromPaecilomyces Bainiersp. 229

Yan

Zhou

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…The fungal species identified by microscopic method and colony characterization were Aspergillus niger (S1), Aspergillus tubingensis (S2), Aspergillus awamori (S3), Aspergillus nidulans (S4), Aspergillus japonicas (S5), Trichoderma reesai (S6), Trichoderma viride (S7), Trichoderma harjianum (S8), and Trichoderma branchianum (S9). Of these species, six fungal isolates were cellulase complex producers in nine samples, and three were genetically determined (Amouri and Gargouri 2006) samples. …”

Section: Isolation Of Fungal Speciesmentioning

confidence: 99%

Hyper-Productivity, Characterization, and Exploitation of a Cellulase Complex from a Novel Isolate of Aspergillus tubingenesis S2 using Lignocellulose-based Material

Imran¹,

Anwar²,

Zafar³

et al. 2017

BioResources

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The hyper-production potential of a cellulase complex from a local strain of Aspergillus tubingensis S2, indigenously isolated from rotten tomato, was investigated. A total of nine fungal species of Aspergillus and Trichoderma were isolated and confirmed through triple-phase screening via 18S ribosomal DNA sequencing and construction of a phylogenetic tree. Congo red testing and the zone of clearance method were used to confirm the cellulase production from A. tubingenesis S2 isolate. A. tubingenesis S2 revealed maximum cellulase production (78 µg/mL/min) and was selected for further study. The optimum fermentative conditions, including the incubation period, pH, and temperature values, were determined to be 96 h, pH 4.8, and 40 °C, respectively, for obtaining the cellulase activity of 86.4±2.1 µg/mL/min. The cellulase was 5.14-fold purified by ammonium sulfate fractionation and gel permeation chromatography. Characterization revealed that maximum activity (130.5 µg/mL/min and 133.5 µg/mL/min) was achieved at 4.5 pH and 40 °C, respectively. A monomeric protein with an apparent molecular weight of 76 kDa was evident after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Cellulase revealed maximal activity with 40-mesh size corn stover as compared with 20-mesh size corn stover and 80-mesh size corn stover after 36 h of incubation at 40 °C.

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“…A análise da termoestabilidade em tampão revelou um tempo de meia vida de 30 min a 60ºC para a β-glucosidase produzida por Sclerotinia sclerotiorum (Issam et al, 2003) (Amouri & Gargouri, 2006;Saibi et al, 2007).…”

Section: A) B)unclassified

“…Similarmente ao observado para a enzima de C. graminicola, diversos estudos mostraram que o pH ótimo de atividade para a maioria das β-glucosidases fúngicas está na faixa de 4,0-6,0 ( Bathia et al, 2002;Issam et al, 2003;Amouri and Gargouri, 2006;Korotkova et al, 2009;Teugjas Väljamäe, 2013).…”

Section: Propriedades Moleculares De Bgcg-2unclassified

“…Por outro lado, a temperatura ótima descrita para as β-glucosidases da maioria dos fungos mesófilos está na faixa de 50-60°C (Karnchanatat et al, 2007;Amouri & Gargouri, 2006;Saibi et al, 2009;Elshafei et al, 2011;Teugjas Väljamäe, 2013), embora as enzimas de P. canescense e Fomitopsis palustris tenham apresentado temperaturas ótimas de 70°C (Dubrovskaya et al, 2011;Yoon, et al, 2008). Já entre os fungos termófilos, os valores de temperaturas ótimas de reação encontram-se entre 50 e 75 °C (Kaur et al, 2007;Yang et al, Venturi et al, 2002;Nascimento et al,2010;Meleiro et al, 2014).…”

Section: Propriedades Moleculares De Bgcg-2unclassified

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Produção e caracterização bioquímica de enzimas lignocelulolíticas fúngicas e sua aplicação na sacarificação de biomassa lignocelulósica

Zimbardi¹

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Characterization of a novel β-glucosidase from a Stachybotrys strain

Cited by 90 publications

References 15 publications

Purification Method Improvement and Characterization of a Novel Ginsenoside-Hydrolyzing β-Glucosidase fromPaecilomyces Bainiersp. 229

Purification Method Improvement and Characterization of a Novel Ginsenoside-Hydrolyzing β-Glucosidase fromPaecilomyces Bainiersp. 229

Hyper-Productivity, Characterization, and Exploitation of a Cellulase Complex from a Novel Isolate of Aspergillus tubingenesis S2 using Lignocellulose-based Material

Produção e caracterização bioquímica de enzimas lignocelulolíticas fúngicas e sua aplicação na sacarificação de biomassa lignocelulósica

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