Characterization of a Novel Gene, srpA, Conferring Resistance to Streptogramin A, Pleuromutilins, and Lincosamides in Streptococcus suis

Zhang, Chaoyang; Liu, Lu; Zhang, Peng; Cui, Jingpo; Qin, Xiao; Ma, Lichao; Han, Kun; Wang, Zhanhui; Wang, Shaolin; Ding, Shuangyang; Shen, Zhangqi

doi:10.1016/j.eng.2020.12.015

Cited by 5 publications

(4 citation statements)

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“…However, we did not detect any Insertion Sequence (IS) sequence upstream and downstream of the resistance gene that could enable formation of a circular form as described by Zhang et al . [62]. However, interestingly, in one strain (strain 1551), the srpA gene is interrupted by a genetic element that encodes a MobQ relaxase (with a PF03389 domain).…”

Section: Resultsmentioning

confidence: 99%

“…[45]. Further analysis of these genes indicated that they encode an ABC-F protein with 84–86 % identity to the recently described ribosome protection protein SrpA [62]. This determinant was shown to confer not only resistance to pleuromutilin but also to lincosamides.…”

Section: Resultsmentioning

confidence: 99%

“…Carriage strains were found to carry more AMR genes than invasive strains. The recently described srpA gene [62], which confers resistance to streptogramins A, lincosamides and to the pleuromutilin tiamulin, was found in 15 strains including two strains isolated from wild boars. Modifications in PBP 1a, PBP 2b, PBP 2x and MraY proteins were found in the penicillin-resistant isolates.…”

Section: Discussionmentioning

confidence: 99%

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Virulence genes, resistome and mobilome of Streptococcus suis strains isolated in France

Dechêne-Tempier,

de Boisséson,

Lucas

et al. 2024

Microbial Genomics

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Streptococcus suis is a leading cause of infection in pigs, causing extensive economic losses. In addition, it can also infect wild fauna, and can be responsible for severe infections in humans. Increasing antimicrobial resistance (AMR) has been described in S. suis worldwide and most of the AMR genes are carried by mobile genetic elements (MGEs). This contributes to their dissemination by horizontal gene transfer. A collection of 102 strains isolated from humans, pigs and wild boars in France was subjected to whole genome sequencing in order to: (i) study their genetic diversity, (ii) evaluate their content in virulence-associated genes, (iii) decipher the mechanisms responsible for their AMR and their association with MGEs, and (iv) study their ability to acquire extracellular DNA by natural transformation. Analysis by hierarchical clustering on principal components identified a few virulence-associated factors that distinguish invasive CC1 strains from the other strains. A plethora of AMR genes (n=217) was found in the genomes. Apart from the frequently reported erm(B) and tet(O) genes, more recently described AMR genes were identified [vga(F)/sprA, vat(D)]. Modifications in PBPs/MraY and GyrA/ParC were detected in the penicillin- and fluoroquinolone-resistant isolates respectively. New AMR gene–MGE associations were detected. The majority of the strains have the full set of genes required for competence, i.e for the acquisition of extracellular DNA (that could carry AMR genes) by natural transformation. Hence the risk of dissemination of these AMR genes should not be neglected.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Virulence genes, resistome and mobilome of Streptococcus suis strains isolated in France

Dechêne-Tempier,

de Boisséson,

Lucas

et al. 2024

Microbial Genomics

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“…The quality of the genome sequences was checked using the default settings in QUAST (version 5.0.2, St. Petersburg Academic University, https://github.com/ablab/quast) (6). Identification of S. suis serotypes was performed using the protocol described by Athey et al (7). Resistance genes were identified using the SRST2 Toolkit (version 0.2.0, The University of Melbourne, http://katholt.github.io/srst2/).…”

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confidence: 99%

Genomic Insight into the Antimicrobial Resistance of <i>Streptococcus Suis</i> — Six Countries, 2011–2019

Ma¹,

Zhang²,

Ma³

et al. 2021

China CDC Weekly

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What is already known on this topic? Streptococcus suis ( S. suis ) is a zoonotic pathogen causing disease in humans and animals, and the emergence of its increased resistance to antimicrobial agents has become a significant challenge in many countries. What is added by this report? Using whole genome sequencing data to accurately predict antimicrobial resistance determinants, it was found that the prevalence of antimicrobial resistance genes was higher in the pig isolates of S. suis than in the human isolates and that the prevalence of these genes varied with serotype. What are the implications for public health practice? The data regarding S. suis antimicrobial resistance will help guide rational drug use in the clinic to better protect the health of humans and animals.

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Demand, status, and prospect of antibiotics detection in the environment

Liu

et al. 2022

Sensors and Actuators B: Chemical

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Characterization of a Novel Gene, srpA, Conferring Resistance to Streptogramin A, Pleuromutilins, and Lincosamides in Streptococcus suis

Cited by 5 publications

References 50 publications

Virulence genes, resistome and mobilome of Streptococcus suis strains isolated in France

Virulence genes, resistome and mobilome of Streptococcus suis strains isolated in France

Genomic Insight into the Antimicrobial Resistance of <i>Streptococcus Suis</i> — Six Countries, 2011–2019

Demand, status, and prospect of antibiotics detection in the environment

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