Virulence genes, resistome and mobilome of Streptococcus suis strains isolated in France

Dechêne-Tempier, Manon; de Boisséson, Claire; Lucas, Pierrick; Bougeard, Stéphanie; Libante, Virginie; Marois-Créhan, Corinne; Payot, Sophie

doi:10.1099/mgen.0.001224

Cited by 2 publications

(2 citation statements)

References 77 publications

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“…Often misclassified in the literature as transposons or plasmids (40), ICEs are also capable of HGT. Studies on enterococci and streptococci showed that these MGEs play a crucial role in the dissemination of ARGs (41). The well-known Tn 916 family was found in many Firmicutes, always carrying the tet(M) gene (42–44).…”

Section: Discussionmentioning

confidence: 99%

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The interplay between Mobilome and Resistome inStaphylococcus aureus

Contarin,

Drapeau,

François

et al. 2024

Preprint

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Antibiotic resistance genes (ARGs) inStaphylococcus aureuscan disseminate vertically through successful clones, but also horizontally through the transfer of genes conveyed by mobile genetic elements (MGEs). The underexplored MGE/ARG associations in S. aureusfavor the emergence of multidrug-resistant clones, posing a significant threat to human and animal health.This study investigated the interplay between the mobilome, encompassing MGEs, and the resistome, the collection of ARGs, in more than 10,000S. aureusgenomes from human and animal sources. The analysis revealed a remarkable diversity of MGEs and ARGs, with plasmids and transposons being the main carriers of resistance genes. Numerous MGE/ARG associations were identified, suggesting that MGEs play a critical role in the dissemination of resistance. A high degree of similaritywas observed in MGE/ARG associations between human and animal isolates, highlighting the potential for unrestricted spread of ARGs between hosts. While clonal expansion is a major driver of resistance dissemination inS. aureus, our results showed that MGEs and their associated ARGs can spread across different strain types (STs), favoring the evolution of these clones and their adaptation in selective environments. The high variability of MGE/ARG associations within individual STs and the ir spread across several STs highlight the crucial role of MGEs in shaping theS. aureusresistome.Overall, this study provides valuable insights into the complex interplay between MGEs and ARGs inS. aureus, emphasizing the need to elucidate the mechanisms governing the epidemic success of MGEs, particularly those implicated in ARG transfer.ImportanceThe research presented in this article highlights the crucial importance of understanding the interactions between mobile genetic elements (MGEs) and antibiotic resistance genes (ARGs) carried byStaphylococcus aureus, a versatile bacterium that can be both a harmless commensal and a dangerous pathogen for humans and animals.S. aureusstrains represent a major threat due to their ability to rapidly acquire and disseminate ARGs. By analyzing a large dataset ofS. aureusgenomes, we highlighted the substantial role of MGEs, in particular plasmids and transposons, in the dissemination of ARGs within and betweenS. aureuspopulations, bypassing the host barrier. Given that multidrug-resistantS. aureusstrains are classified as a high-prioritypathogenby global health organizations, this knowledge is crucial for understanding the complex dynamics of transmission of antibiotic resistance in this species.

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Section: Discussionmentioning

confidence: 99%

“…Studies on enterococci and streptococci showed that these MGEs play a crucial role in the dissemination of ARGs (41). The well-known Tn916 family was found in many Firmicutes, always carrying the tet(M) gene (42)(43)(44).…”

Section: Discussionmentioning

confidence: 99%

The interplay between Mobilome and Resistome inStaphylococcus aureus

Contarin,

Drapeau,

François

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

The interplay between mobilome and resistome in Staphylococcus aureus

Contarin,

Drapeau,

François

et al. 2024

mBio

View full text Add to dashboard Cite

Antibiotic resistance genes (ARGs) in Staphylococcus aureus can disseminate vertically through successful clones, but also horizontally through the transfer of genes conveyed by mobile genetic elements (MGEs). Even though underexplored, MGE/ARG associations in S. aureus favor the emergence of multidrug-resistant clones, which are challenging therapeutic success in both human and animal health. This study investigated the interplay between the mobilome and the resistome of more than 10,000 S . aureus genomes from human and animal origin. The analysis revealed a remarkable diversity of MGEs and ARGs, with plasmids and transposons being the main carriers of ARGs. Numerous MGE/ARG associations were identified, suggesting that MGEs play a critical role in the dissemination of resistance. A high degree of similarity was observed in MGE/ARG associations between human and animal isolates, highlighting the potential for unrestricted spread of ARGs between hosts. Our results showed that in parallel to clonal expansion, MGEs and their associated ARGs can spread across different strain types sequence types (STs), favoring the evolution of these clones and their adaptation in selective environments. The high variability of MGE/ARG associations within individual STs and their spread across several STs highlight the crucial role of MGEs in shaping the S. aureus resistome. Overall, this study provides valuable insights into the complex interplay between MGEs and ARGs in S. aureus , emphasizing the need to elucidate the mechanisms governing the epidemic success of MGEs, particularly those implicated in ARG transfer. IMPORTANCE The research presented in this article highlights the importance of understanding the interactions between mobile genetic elements (MGEs) and antibiotic resistance genes (ARGs) carried by Staphylococcus aureus , a versatile bacterium that can be both a harmless commensal and a dangerous pathogen for humans and animals. S. aureus has a great capacity to acquire and disseminate ARGs, enabling efficient adaption to various environmental or clinical conditions. By analyzing a large data set of S. aureus genomes, we highlighted the substantial role of MGEs, particularly plasmids and transposons, in disseminating ARGs within and between S. aureus populations, bypassing host barriers. Given that multidrug-resistant S. aureus strains are classified as a high-priority pathogen by global health organizations, this knowledge is crucial for understanding the complex dynamics of transmission of antibiotic resistance in this species.

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Virulence genes, resistome and mobilome of Streptococcus suis strains isolated in France

Cited by 2 publications

References 77 publications

The interplay between Mobilome and Resistome inStaphylococcus aureus

The interplay between Mobilome and Resistome inStaphylococcus aureus

The interplay between mobilome and resistome in Staphylococcus aureus

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