Characterization of a new T2DS.2DL-?R translocation triticale ZH-1 with multiple resistances to diseases

Zhou, Jianping; Zhang, Huaiyu; Yang, Zujun; Li, Chang-Jiu; Hu, Lijun; Lei, Minjie; Liu, Cheng; Yong, Zhang; Ren, Zhenglong

doi:10.1007/s10722-011-9751-0

Cited by 17 publications

(18 citation statements)

References 35 publications

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“…The conclusion is that these lines retained most of the A-, B-and R-genome chromosomes, while most of the D-genome chromosomes were eliminated. A similar phenomenon was observed in previous studies of Nakata et al [1984], Dou et al [2006] and Zhou et al [2012]. They found that hexaploid lines could be spontaneously derived from primary octoploid triticales, with the retention of complete sets of A-and Bgenome chromosomes and most R-genome chromosomes, and the elimination of most D-genome chromosomes.…”

Section: Discussionsupporting

confidence: 87%

“…Secondary hexaploid triticales were also synthesized by hybridizing hexaploid triticale and/or hexaploid wheat with an octoploid triticale [Ma and Gustafson, 2008]. In addition, many hexaploid derivatives can spontaneously appear in the selfed progenies of octoploid triticale, with the elimination of the D genome [Nakata et al, 1984;Dou et al, 2006;Zhou et al, 2012]. Recently, Hao et al [2013] reported that complete hexaploid triticales with 28 intact A/B and 14 intact R chromosomes could be effectively produced via hybridization of synthetic hexaploid wheat with rye.…”

Section: Discussionmentioning

confidence: 99%

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Cytogenetic Behavior of Trigeneric Hybrid Progeny Involving Wheat, Rye and Psathyrostachys huashanica

Kang

Huang²,

Zhu³

et al. 2016

Cytogenet Genome Res

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Trigeneric hybrids are commonly used as bridges to transfer genes from some wild species to cultivated wheat and to measure the genomic interaction between donor species. We previously reported that trigeneric germplasms were produced by crossing wheat-Psathyrostachys huashanica amphiploids (PHW-SA, 2n = 8x = 56, AABBDDNsNs) with hexaploid triticale (Zhongsi 828, 2n = 6x = 42, AABBRR). In the present study, chromosome pairing behavior and the genome constitution of the F₄ progenies of wheat-rye-P. huashanica trigeneric hybrids were studied. Cytological analysis showed that the chromosome number of F₄ progenies ranged from 39 to 46, and 57.5% of them had 42 chromosomes. The mean meiotic configuration of F₄ lines was 1.71 univalents, 20.26 bivalents, 0.04 trivalents, and 0.001 quadrivalents per pollen mother cell. Among the lines with 2n = 42, the average pairing configuration was 1.21 univalents, 16.22 ring bivalents, 4.16 rod bivalents, and 0.01 trivalents. This result indicated that these lines were cytologically stable. Other lines with 2n = 39, 40, 41, 43, 44, 45, and 46, bearing a high number of univalents or multivalents, showed abnormal meiotic behavior. Genomic in situ hybridization (GISH) revealed that all F₄ lines had 11-14 rye chromosomes, but no P. huashanica chromosomes. The complete set of 14 rye chromosomes was found in 19 lines. At meiosis, GISH detected 1-6 univalents with hybridization signals of rye in 13 lines. Bivalents with fluorescence signals were identified in each line, ranging from 3 to 7. A quadrivalent with hybridization signals was observed in only 1 line, K13-714-8. Lagging chromosomes, chromosome bridges, micronuclei, and chromosome fragments hybridizing with the probe were not discovered in any of the lines. These results inferred that the behavior of rye chromosomes was normal during meiosis. In addition, 21 lines of 2n = 42 (91.3%) with 12 or 14 rye chromosomes, always contained 6 or 7 bivalents bearing fluorescence signals. This suggested that the rye chromosomes exhibiting complete pairing in these lines were cytologically stable during meiosis and may therefore be considered as new hexaploid triticales. Thus, these lines might be potential materials for further hexaploid triticale improvement.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Cytogenetic Behavior of Trigeneric Hybrid Progeny Involving Wheat, Rye and Psathyrostachys huashanica

Kang

Huang²,

Zhu³

et al. 2016

Cytogenet Genome Res

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show abstract

“…Somatic chromosome counts and meiosis analysis were done as described previously (Zhou et al 2012). Root tips and the chromosome spreads of materials were prepared according to Han et al (2006).…”

Section: Cytological Observationsmentioning

confidence: 99%

“…biuncialis, and 12-5-1 were extracted as described previously (Zhou et al 2012). A total of 48 pair primers of PLUG markers, including 22 that were group 1 primers in Ishikawa et al (2009) and 26 (TNAC1-01, TNAC1-02, TNAC1-26) that were designed by us, were used in this study.…”

Section: Plug Marker Analysismentioning

confidence: 99%

Characterization of a New Wheat-Aegilops biuncialis1M^b(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit

Zhou¹,

Cheng²,

Zang³

et al. 2016

Cereal Research Communications

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In this study, a new substitution line, 12-5-1, with 42 chromosomes that was derived from BC 3 F 2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. The 12-5-1 was immune to both powdery mildew and stripe rust and has stable fertility. Multi-color fluorescence in situ hybridization indicated that 12-5-1 was a substitution line 1M b (1B). The seed storage protein electrophoresis showed that 12-5-1 presented high molecular weight glutenin subunits (2 + 12) of CN19 and a new subunit designated as M which apparently originated from parent Ae. biuncialis, and absent 7 + 8 subunits. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness and mixing time of 12-5-1 were signifiantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1M b -specifi polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1026, TNAC1041, TNAC1-02 and TNAC1-04, were also obtained. The new substitution line 1M b (1B) 12-5-1 could be a valuable source for wheat improvement, especially for wheat end product quality and resistance to disease.

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“…Total genomic DNA from wheat materials CS, CN19, A. biuncialis, and 12-5-2 were extracted as described previously (Zhou et al, 2012). A total of 48 pairs of PLUG markers, including 22 that were group 1 primers in Ishikawa et al (2009) and 26 (TNAC1-01, TNAC1-02,…, TNAC1-26) that were designed by us, were used in this study.…”

Section: Plug Marker Analysismentioning

confidence: 99%

Characterization of a new wheat-Aegilops biuncialis addition line conferring quality-associated HMW glutenin subunits

Zhou¹,

Yao²,

Yang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. In this study, a new disomic addition line, 12-5-2, with 44 chromosomes that was derived from BC 3 F 2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. 12-5-2 was immune to both powdery mildew and stripe rust and has stable fertility. Fluorescence in situ hybridization and C-banding revealed that 12-5-2 was a 1U b disomic addition line (ADL1U b ). The seed storage protein electrophoresis showed that 12-5-2 presented all high molecular weight glutenin subunits (7 + 8 and 2 + 12) of CN19 and 2 new subunits that were designated Ux and Uy. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness of 12-5-2 were significantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1U b -specific polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1041, TNAC1071, TNAC1-01, and TNAC1-04, were also obtained. The new ADL1U b 12-5-2 could be a valuable source for wheat improvement, especially for wheat endproduct quality and resistance to disease.

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Characterization of a new T2DS.2DL-?R translocation triticale ZH-1 with multiple resistances to diseases

Cited by 17 publications

References 35 publications

Cytogenetic Behavior of Trigeneric Hybrid Progeny Involving Wheat, Rye and <b><i>Psathyrostachys huashanica</i></b>

Cytogenetic Behavior of Trigeneric Hybrid Progeny Involving Wheat, Rye and <b><i>Psathyrostachys huashanica</i></b>

Characterization of a New Wheat-Aegilops biuncialis1M^b(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit

Characterization of a new wheat-Aegilops biuncialis addition line conferring quality-associated HMW glutenin subunits

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Characterization of a new T2DS.2DL-?R translocation triticale ZH-1 with multiple resistances to diseases

Cited by 17 publications

References 35 publications

Cytogenetic Behavior of Trigeneric Hybrid Progeny Involving Wheat, Rye and <b><i>Psathyrostachys huashanica</i></b>

Cytogenetic Behavior of Trigeneric Hybrid Progeny Involving Wheat, Rye and <b><i>Psathyrostachys huashanica</i></b>

Characterization of a New Wheat-Aegilops biuncialis1Mb(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit

Characterization of a new wheat-Aegilops biuncialis addition line conferring quality-associated HMW glutenin subunits

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Characterization of a New Wheat-Aegilops biuncialis1M^b(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit