Characterization of a New Wheat-Aegilops biuncialis1M^b(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit

Abstract

In this study, a new substitution line, 12-5-1, with 42 chromosomes that was derived from BC 3 F 2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. The 12-5-1 was immune to both powdery mildew and stripe rust and has stable fertility. Multi-color fluorescence in situ hybridization indicated that 12-5-1 was a substitution line 1M b (1B). The seed storage protein electrophoresis showed that 12-5-1 presented high molecular weight glutenin subunits (2 + 12) of CN19 and a new subunit designated as M which apparently originated from parent Ae. biuncialis, and absent 7 + 8 subunits. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness and mixing time of 12-5-1 were signifiantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1M b -specifi polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1026, TNAC1041, TNAC1-02 and TNAC1-04, were also obtained. The new substitution line 1M b (1B) 12-5-1 could be a valuable source for wheat improvement, especially for wheat end product quality and resistance to disease.

Disomic 1M (1B) Triticum aestivum–Aegilops comosa Substitution Line with Favorable Protein Properties

“…For example, the Mx subunit transferred from the 1M b chromosome of Ae. biuncialis into wheat could significantly increase the protein content, wet gluten content, sedimentation value, and grain hardness . The 1M g chromosomes introduced from Ae.…”

“…biuncialis contains new highmolecular-weight glutenin subunits (HMW-GSs), endowing wheat with good bread-making quality. 3 The 1M g chromosome from Ae. geniculata contains genes that increase the proportion of polymeric gluten proteins.…”

“…For example, the 1M b chromosome from Ae. biuncialis contains new high-molecular-weight glutenin subunits (HMW-GSs), endowing wheat with good bread-making quality . The 1M g chromosome from Ae.…”

Identification of New QTLs for Dietary Fiber Content in Aegilops biuncialis

“…To introduce favorable agronomic traits of Aegilops , some U b - and M b -genome chromosomes were transferred into wheat by the development of wheat- Ae. biuncialis amphiploids, disomic addition lines carrying chromosomes 1U b , 2U b , 3U b , 2M b , 3M b and 7M b , a double disomic addition line 1U b and 6U b , 3M b (4B) substitution, and 3M b .4BS translocation lines [ 30 , 34 , 35 , 36 , 37 , 38 ].…”

Grain quality indices in common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis.

“…Фактори експериментальної еволюції організмів 2021. Том 29 cialis [7]. Заміна хромосоми 1B на 1S l від Aegilops longissima у сорту пшениці м'якої Chinese Spring підвищувала хлібопекарну якість -показник SDS седиментації на 44 % і об'єм хліба на 27 % [8].…”