Characterization of a new wheat-Aegilops biuncialis addition line conferring quality-associated HMW glutenin subunits

Abstract

ABSTRACT. In this study, a new disomic addition line, 12-5-2, with 44 chromosomes that was derived from BC 3 F 2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. 12-5-2 was immune to both powdery mildew and stripe rust and has stable fertility. Fluorescence in situ hybridization and C-banding revealed that 12-5-2 was a 1U b disomic addition line (ADL1U b ). The seed storage protein electrophoresis showed that 12-5-2 presented all high molecular weight glutenin subunits (7 + 8 and 2 + 12) of CN19 and 2 new subunits that were designated Ux and Uy. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness of 12-5-2 were significantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1U b -specific polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1041, TNAC1071, TNAC1-01, and TNAC1-04, were also obtained. The new ADL1U b 12-5-2 could be a valuable source for wheat improvement, especially for wheat endproduct quality and resistance to disease.

Transfer of HMW glutenin subunits from Aegilops kotschyi to wheat through radiation hybridization

“…Addition of alien HMWGS in wheat-Ae. biuncialis addition line also enhanced the SDS Sedimentation value (Zhou et al 2014). The micro-sedimentation test of the PRH derivatives indicated that the addition of 1U specific HMWG subunits increased the MST values only in the presence of Glu-1D as their absence in PRH B-58-3 derivative gave extremely low MST values.…”

“…biuncialis addition lines have been used for the transfer of HMW glutenin subunits from Ae. biuncialis to wheat through induced homoeologous pairing (Zhou et al 2014) while wheat-Ae. kotschyi addition lines were used for enhancement of grain protein, Fe and Zn content (Verma et al 2016a).…”

Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113

“…Thus far, plenty of wheat-alien addition lines derived from multiple species of Triticeae have been obtained. These Triticeae species include Hordeum vulgare L. [4–7], Secale cereale L. [8,9], Aegilops speltoides [10,11] and Haynaldia villosa [12,13] and so on. However, most wheat-alien disomic addition lines could not be directly used in wheat breeding due to the presence of linkage drag.…”

Genetic and epigenetic alterations induced by different levels of rye genome integration in wheat recipient

“…The introduction of an alien genetic germplasm is a common method for genetic improvement of wheat. Many wild relatives with important agronomic traits, such as rye (Secale cereale L.), Haynaldia villosa, and Elytrigia elongata, are ideal materials for use in wheat genetic breeding (Qi et al, 2007;Gill et al, 2011;Kang et al, 2011;Liu et al, 2011;Jauhar and Peterson, 2013;Zhou et al, 2014). Rye is a valuable source of resistance, stress tolerance, and high yield genes, and it has been extensively used for yield improvement, disease resistance, and environmental adaptation of common wheat (Friebe et al, 1996;Tang et al, 2009;Ren et al, 2011).…”