Characterization of a High-Virulence US Isolate of Porcine Reproductive and Respiratory Syndrome Virus in a Continuous Cell Line, ATCC CRL11171

Meng, Xiang‐Jin; Paul, P. S.; Halbur, Patrick G.; Lum, Melissa A.

doi:10.1177/104063879600800317

Cited by 62 publications

(68 citation statements)

References 25 publications

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“…Whether this difference is related to the higher virulence of VR2385 is not known at this time. The VR2385 strain has been shown to produce more severe pneumonia and replicate to a higher titre in pigs than LV (Halbur et al, 1996). Another major finding was that most of the apoptotic cells in both in vitro and in vivo studies were uninfected bystander cells.…”

Section: Cjjb T Sirinarumitr and Others T Sirinarumitr And Othersmentioning

confidence: 77%

“…Therefore, PRRSV-induced apoptosis in bystander cells might be the mechanism for amplifying the CPE of PRRSV. Differences in virulence of PRRSV isolates have also been demonstrated (Halbur et al, 1996). Since the virulence of PRRSV varies considerably among isolates, it is possible that the ability of PRRSV to induce apoptosis in bystander cells may be related to virus virulence.…”

Section: Cjjb T Sirinarumitr and Others T Sirinarumitr And Othersmentioning

confidence: 96%

“…ATCC CRL11171 cells (Meng et al, 1996) were inoculated with ATCC VR2385 strain of PRRSV (10' n & TCID &! \ml) at an m.o.i.…”

Section: Evidence Of Apoptosis Was Detected For the Unitedmentioning

confidence: 99%

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A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo.

Sirinarumitr

Zhang

Kluge

et al. 1998

Journal of General Virology

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Evidence of apoptosis was detected for the UnitedStates porcine reproductive and respiratory syndrome virus (PRRSV) in ATCC CRL11171 cells inoculated with strain ATCC VR2385 and in the tissues of pigs infected with the same strain. Apoptosis was detected by agarose gel electrophoresis, transmission electron microscopy and terminal deoxytransferase dUTP nick end labelling (TUNEL) techniques. By electron microscopy and double-labelling techniques, apoptosis was detected primarily in uninfected bystander cells in the continuous cell line rather than the PRRSV-infected cells. In the lungs, the apoptotic cells were predominantly alveolar and pulmonary intravascular macrophages, and mononuclear cells in the alveolar septa. In the lymph nodes, the apoptotic cells were predominantly tingible body macrophages and mononuclear cells. The induction of apoptosis in a large number of mononuclear cells in the lungs and lymph nodes appears to be a mechanism of PRRSV pathogenesis and might be an explanation for a dramatic reduction in the number of alveolar macrophages and circulating lymphocytes and monocytes in PRRSV-infected pigs.Porcine reproductive and respiratory syndrome (PRRS), which has been recognized in the United States (US) since 1987 (Hill, 1990), is characterized by reproductive failure (stillborn, mummified and weak-born piglets) and respiratory disease in

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Section: Cjjb T Sirinarumitr and Others T Sirinarumitr And Othersmentioning

confidence: 77%

Section: Cjjb T Sirinarumitr and Others T Sirinarumitr And Othersmentioning

confidence: 96%

See 1 more Smart Citation

A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo.

Sirinarumitr

Zhang

Kluge

et al. 1998

Journal of General Virology

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“…During the in vivo study, M. hyopneumoniae was isolated from the bronchial swab specimens obtained at necropsy, as described previously (24). PRRSV isolation and titration were performed with BAL fluid collected at necropsy and with the culture supernatant at 6 and 15 hpi by an established protocol (19,27). Briefly, monolayers of MARC-145 cells were incubated with the samples, followed by staining with anti-PRRSV monoclonal antibody SDOW-17 (South Dakota State University, Brookings) and fluorescein isothiocyanate-conjugated anti-mouse immunoglobulin.…”

Section: Methodsmentioning

confidence: 99%

Increased Production of Proinflammatory Cytokines following Infection with Porcine Reproductive and Respiratory Syndrome Virus andMycoplasma hyopneumoniae

Thanawongnuwech

Thacker

Halbur

et al. 2004

Clin Vaccine Immunol

151

107

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“…It has been documented that PRRSV strains differ in virulence (14)(15)(16)30) and vary genetically (23)(24)(25)(26)(27). Concerns that vaccine strains or derivatives of the vaccine strains may induce disease continue to be discussed (20,28,36).…”

mentioning

confidence: 99%

Comparison of Molecular and Biological Characteristics of a Modified Live Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Vaccine (Ingelvac PRRS MLV), the Parent Strain of the Vaccine (ATCC VR2332), ATCC VR2385, and Two Recent Field Isolates of PRRSV

Opriessnig

Halbur

Yoon

et al. 2002

J Virol

148

100

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The objectives of this study were to compare the molecular and biological characteristics of recent porcine reproductive and respiratory syndrome virus (PRRSV) field isolates to those of a modified live virus (MLV) PRRS vaccine and its parent strain. One hundred seventeen, 4-week-old pigs were randomly assigned to six groups. Group 1 (n ‫؍‬ 20) served as sham-inoculated negative controls, group 2 (n ‫؍‬ 19) was inoculated with Ingelvac PRRS MLV vaccine, group 3 (n ‫؍‬ 20) was inoculated with the parent strain of the vaccine (ATCC VR2332), group 4 (n ‫؍‬ 19) was inoculated with vaccine-like PRRSV field isolate 98-38803, group 5 (n ‫؍‬ 19) was inoculated with PRRSV field isolate 98-37120, and group 6 (n ‫؍‬ 20) was inoculated with known high-virulence PRRSV isolate ATCC VR2385. The levels of severity of gross lung lesions (0 to 100%) among the groups were significantly different at both 10 (P < 0.0001) and 28 days postinoculation (p.

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Characterization of a High-Virulence US Isolate of Porcine Reproductive and Respiratory Syndrome Virus in a Continuous Cell Line, ATCC CRL11171

Cited by 62 publications

References 25 publications

A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo.

A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo.

Increased Production of Proinflammatory Cytokines following Infection with Porcine Reproductive and Respiratory Syndrome Virus andMycoplasma hyopneumoniae

Comparison of Molecular and Biological Characteristics of a Modified Live Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Vaccine (Ingelvac PRRS MLV), the Parent Strain of the Vaccine (ATCC VR2332), ATCC VR2385, and Two Recent Field Isolates of PRRSV

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