Characterization of a cationic surfactant-resistant mutant isolated spontaneously from Escherichia coli

Ishikawa, Shu; Matsumura, Yoshitaka; Yoshizako, Fumiki; Tsuchido, Tetsuaki

doi:10.1046/j.1365-2672.2002.01526.x

Cited by 45 publications

(45 citation statements)

References 41 publications

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“…Observed changes in Omps and in particular the upregulation of the small porin OmpC and the downregulation of the larger porin OmpF is well described in the literature as a mechanism to reduce access through the cell membrane (Ishikawa et al, 2002). The Taqman results for ompF, which showed a normalization of the ompF level after an initial decrease in expression level for one of the adapted lines, could indicate that the ompF response is not critical for the BC-resistant strain.…”

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confidence: 61%

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Adapted tolerance to benzalkonium chloride in Escherichia coli K-12 studied by transcriptome and proteome analyses

Bore

Hébraud²,

Chafsey³

et al. 2007

Microbiology

100

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Benzalkonium chloride (BC) is a commonly used disinfectant and preservative. This study describes changes in expression level at the transcriptomic and proteomic level for Escherichia coli K-12 gradually adapted to a tolerance level to BC of 7-8 times the initial MIC. Results from DNA arrays and two-dimensional gel electrophoresis for global gene and protein expression studies were confirmed by real-time quantitative PCR. Peptide mass fingerprinting by MALDI-TOF MS was used to identify differentially expressed proteins. Changes in expression level in adapted cells were shown for porins, drug transporters, glycolytic enzymes, ribosomal subunits and several genes and proteins involved in protection against oxidative stress and antibiotics. Adapted strains showed increased tolerance to several antibiotics. In conclusion, E. coli K-12 adapted to higher tolerance to BC acquired several general resistance mechanisms, including responses normally related to the multiple antibiotic resistance (Mar) regulon and protection against oxidative stress. The results revealed that BC treatment might result in superoxide stress in E. coli. INTRODUCTIONQuaternary ammonium compounds (QACs) such as benzalkonium chloride (BC) and cetrimide are frequently used for disinfection and preservation. QACs are relatively stable, non-corrosive compounds with low toxicity and efficacy over a wide pH range. The destructive mechanism of QACs against bacteria is still not known in detail, but is thought to involve a general perturbation of the lipid bilayer in bacterial membranes. This leads to leakage of cytoplasmic material, damaging and ultimately killing the bacterial cell. For a review see Gilbert & Moore (2005). The QACs have been actively deployed since the 1930s and one has not seen a resistance development similar to that seen for antibiotics in the same period. Still, there are numerous reports of QAC resistance (Heir et al., 1999;Langsrud et al., 2003;Wright & Gilbert, 1987), most often associated with acquisition, or hyperexpression, of multi-drug efflux pumps (Li & Nikaido, 2004;Tikhonova & Zgurskaya, 2004), which has also been associated with changes in MIC of therapeutically important antibiotics serving as substrates to such pumps (Langsrud et al., 2004;Poole, 2004Poole, , 2005. Because of their relatively impermeable outer membrane, Gram-negative bacteria are intrinsically more resistant to QACs than Gram-positives (McDonnell & Russell, 1999), but both Gram-negative and Gram-positive BC-resistant bacteria have been isolated from the food industry (Aase et al., 2000;Heir et al., 1995;Langsrud et al., 2003;Soumet et al., 2005).Several studies have linked intrinsic/natural resistance of Gram-negative bacteria to tenside-based disinfectants, such as QACs, to the low permeability of the outer membrane, or broad-spectrum efflux systems (Denyer & Maillard, 2002;Nikaido, 2001). Acquisition of resistance in Escherichia coli has been related mainly to changes in the composition of lipopolysaccharide (LPS) and fatty acids in the membrane (...

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confidence: 61%

“…Acquisition of resistance in Escherichia coli has been related mainly to changes in the composition of lipopolysaccharide (LPS) and fatty acids in the membrane (Ishikawa et al, 2002;Sakagami et al, 1989). In contrast to antibiotics, which have very specific targets within the bacterial cell, biocides affect multiple cellular components.…”

Section: Introductionmentioning

confidence: 99%

Adapted tolerance to benzalkonium chloride in Escherichia coli K-12 studied by transcriptome and proteome analyses

Bore

Hébraud²,

Chafsey³

et al. 2007

Microbiology

100

View full text Add to dashboard Cite

show abstract

“…Isolate S-2-6 was more closely related to an antibiotic-resistant strain of Pseudomonas fluorescens c6 [42]. It is known that bacteria resistant to surfactants are frequently also resistant to antibiotics [33] and vice-versa [51]; that is, because resistance mechanisms, such as changes in membrane composition and efflux pumps, are similar.…”

Section: Pseudomonads Among Surfactant-resistant Bacterioneuston Isolmentioning

confidence: 99%

Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer

Louvado

Coelho²,

Domingues³

et al. 2012

J. Microbiol. Biotechnol.

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“…and rhamnolipid biosurfactant of P. aeruginosa AT10 by Haba et al (37). Relying on therapeutic applications of some biosurfactants as antibiotics and antifungal or antiviral compounds, some of researchers studied on several surfactant-resistant strains and isolated sensitive strains of bacteria by artificial or spontaneous mutagenesis and concerned this phenomenon to possess altered structures and functions in their cell membranes (38,39).…”

Section: Bagheri Lotfabad T Et Al Antibacterial Capabilities Of Rhammentioning

confidence: 99%

Assessment of Antibacterial Capability of Rhamnolipids Produced by Two Indigenous Pseudomonas aeruginosa Strains

Lotfabad

Shahcheraghi

Shooraj

2012

Jundishapur J Microbiol

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This study shows that rhamnolipid mixtures of MR01 and MASH1 have antimicrobial efficacy against Gram positive bacteria and may be evaluated as antimicrobial agent against multidrugresistant clinical pathogenic isolates. Background:In recent decades, biological properties of biosurfactants, particularly glycolipids, including antimicrobial, antifungal, and anti-cellular effects have been projected in many research studies. The unique advantages of these compounds in terms of production and environment made them important as antimicrobial agents. Objectives: This study aims to evaluate probable antibacterial and antifungal properties of MR01 and MASH1 biosurfactants against several specified microorganisms. Materials and Methods:In this article antibacterial activities of two rhamnolipid mixtures of MR01 and MASH1 were studied. MR01 and MASH1 are glycolipid-type biosurfactants that are being produced by two strains of Pseudomonas aeruginosa MR01 and MASH1, respectively. Antibacterial effects of two biosurfactants were assessed by disc diffusion test method and determination of minimum inhibitory concentration (MIC). Results: They exhibit not only excellent surface activity but also remarkable inhibitory effect against Gram-positive bacteria. According to results, although none of two biosurfactants showed significant effects on Gram negative bacteria growth inhibition, both assessing methods confirmed the growth inhibition of Gram-positive bacteria by MR01 and MASH1 biosurfactants. Conclusions: According to results of this study, MR01 and MASH1 biosurfactants had antimicrobial efficacy against Gram positive bacterial groups. This effect is comparable to antibiotics and therefore the future use of these biosurfactants as broad spectrum antibiotics is highly promising. These features of biosurfactants (BS) should broaden its applications in new advanced technologies. Future studies will be performed on characterization and isolation of biologically active fraction of the rhamnolipid biosurfactant produced by P. aeruginosa strains. This bioactive compound may be evaluated as a potent antimicrobial agent to be applied against a panel of pathogenic micro-organisms including a few multidrug-resistant (MDR) clinical pathogenic isolates such as methicillin-resistant Staphylococcus aureus (MRSA) and other MDR pathogenic strains.

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Characterization of a cationic surfactant-resistant mutant isolated spontaneously from Escherichia coli

Cited by 45 publications

References 41 publications

Adapted tolerance to benzalkonium chloride in Escherichia coli K-12 studied by transcriptome and proteome analyses

Adapted tolerance to benzalkonium chloride in Escherichia coli K-12 studied by transcriptome and proteome analyses

Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer

Assessment of Antibacterial Capability of Rhamnolipids Produced by Two Indigenous Pseudomonas aeruginosa Strains

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