Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer

Louvado, António; Coelho, Francisco J. R. C.; Domingues, Patrícia M.; Santos, A. L. Q.; Gomes, Newton C. M.; Almeida, Adelaide

doi:10.4014/jmb.1110.10041

Cited by 18 publications

(13 citation statements)

References 63 publications

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“…Part of this observed drift is likely because of thread debris, as discussed in section Overview and Identification of Outliers . One possible additional source of drift is microbial growth: despite the ability of SDS to denature proteins, bacteria have been observed to degrade SDS at concentrations in excess of the 0.1% mass concentration used here . Subsequent to the data acquisition phase of the comparison, a stability test was initiated on ETFE particle suspensions of similar particle size distribution, but with the addition of 0.02% mass concentration of sodium azide and with greater care in filtration of the buffer.…”

Section: Methodsmentioning

confidence: 99%

“…One possible additional source of drift is microbial growth: despite the ability of SDS to denature proteins, bacteria have been observed to degrade SDS at concentrations in excess of the 0.1% mass concentration used here. 21,22 Subsequent to the data acquisition phase of the comparison, a stability test was initiated on ETFE particle suspensions of similar particle size distribution, but with the addition of 0.02% mass concentration of sodium azide and with greater care in filtration of the buffer. The stability of this second lot over 6.5 months was a factor of 1.9 better than the stability of the comparison lot over 3 months (data not shown).…”

Section: Variability Of the Particle Concentrationmentioning

confidence: 99%

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An Interlaboratory Comparison of Sizing and Counting of Subvisible Particles Mimicking Protein Aggregates

Ripple

Montgomery

2015

Journal of Pharmaceutical Sciences

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Section: Methodsmentioning

confidence: 99%

Section: Variability Of the Particle Concentrationmentioning

confidence: 99%

An Interlaboratory Comparison of Sizing and Counting of Subvisible Particles Mimicking Protein Aggregates

Ripple

Montgomery

2015

Journal of Pharmaceutical Sciences

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“…The microorganisms used in this study were the Gram‐positive bacteria Staphylococcus aureus (ATCC 700699), an environmental strain of the Gram‐negative bacteria Pseudomonas aeruginosa and the yeast Candida albicans (ATCC 10231). These microorganisms were selected because of the clinical and environmental relevance of their biofilms .…”

Section: Methodsmentioning

confidence: 99%

Photodynamic Inactivation of Bacterial and Yeast Biofilms With a Cationic Porphyrin

Beirão

Fernandes

Coelho

et al. 2014

Photochem & Photobiology

109

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The efficiency of 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin tetra-iodide (Tetra-Py(+)-Me) in the photodynamic inactivation of single-species biofilms of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans and mixed biofilms of S. aureus and C. albicans was evaluated. The effect on the extracellular matrix of P. aeruginosa was also assessed. Irradiation with white light up to an energy dose of 64.8 J cm(-2) in the presence of 20 μm of Tetra-Py(+)-Me caused significant inactivation in all single-species biofilms (3-6 log reductions), although the susceptibility was attenuated in relation to planktonic cells. In mixed biofilms, the inactivation of S. aureus was as efficient as in single-species biofilms but the susceptibility of C. albicans decreased. In P. aeruginosa biofilms, a reduction of 81% in the polysaccharide content of the matrix was observed after treatment with a 20 μm PS concentration and a total light dose of 64.8 J cm(-2). The results show that the Tetra-Py(+)-Me causes significant inactivation of the microorganisms, either in biofilms or in the planktonic form, and demonstrate that polysaccharides of the biofilm matrix may be a primary target of photodynamic damage.

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“…However, the presence of SDS or CTAC had negative effects on B. megaterium ureolytic activity. It has been known that membranes with low unsaturated fatty acid contents in phospholipids are more sensitive to SDS (Bartnik 1992) while anionic surfactants are more effective against Gram-positive bacteria than the Gramnegatives (Louvado et al 2012). Although both B. megaterium and S. pasteurii are Gram-positive, vegetative cell wall peptidoglycan of S. pasteurii contains lysine instead of meso-diaminopimelic acid (m-DAP) (Slepecky and Hemphill 2006).…”

Section: Effect Of Surfactants On Microbial Activitymentioning

confidence: 99%

Effect of Surfactants on CO2 Biomineralization with Sporosarcina pasteurii and Bacillus megaterium

Cho

Mahanty

Kim

2014

Water Air Soil Pollut

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Surfactant can reduce the interfacial tension in liquid-gas system and may probably improve the rate and/or extent of dissolution. This study was conducted to evaluate the effect of three different surfactants (viz., sodium dodecyl sulfate (SDS), Triton X-100, and cetyltrimethylammonium chloride (CTAC)) on CO 2 biomineralization by two ureolytic microorganismSporosarcina pasteurii and Bacillus megaterium. In S. pasteurii-mediated biomineralization, headspace CO 2 content (2.5 mM) was decreased by 40, 52, and 68 % in the presence of SDS, Triton X-100 or CTAC, respectively within the first 8 h of incubation. CO 2 removal with B. megaterium in the presence of Triton X-100 (64 %) and CTAC (56 %) was better in comparison to control without surfactant (48 %). However, appreciable CO 2 depletion was not observed with SDS, which was just 4 %. On other hand, headspace CO 2 loss in the presence of CTAC with B. megaterium did not get biomineralized, as no calcium carbonate was detected. Crystalline phase and morphology of CaCO 3 precipitate also varied between ionic and nonionic surfactants. The result suggests that the effect of surfactant on CO 2 capture and biomineralization can be largely different, depending on the surfactant and concerned microbial species involved.

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Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer

Cited by 18 publications

References 63 publications

An Interlaboratory Comparison of Sizing and Counting of Subvisible Particles Mimicking Protein Aggregates

An Interlaboratory Comparison of Sizing and Counting of Subvisible Particles Mimicking Protein Aggregates

Photodynamic Inactivation of Bacterial and Yeast Biofilms With a Cationic Porphyrin

Effect of Surfactants on CO2 Biomineralization with Sporosarcina pasteurii and Bacillus megaterium

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